LOCUS       sequence-252545-        4059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5107
FEATURES             Location/Qualifiers
     source          1..4059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1505..1604)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1621..1640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1622..1640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1645..1661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1645..1661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1763..1782)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1774..2583
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1841..1860)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2730..3318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3219..3238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3472..3489
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3648..3675)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3767..3853)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3902..3924
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3916..3933
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3917..3933
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3949..4048
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgcacatca accgacgcgt ccaacaaccg
       61 cctgtgactg cgacggatag ctttcggaca gcgtccgacg cgtctcttgc ctccagctct
      121 gtgcgatctg tcagctccga tcagcaacgc gagataaatg cgattgccga ttacctgaca
      181 gatcatgtgt tcgctgcgca taaactgccg ccggccgatt cggctgatgg ccaagctgca
      241 gttgacgtac acaatgcgca gatcactgcg ctgatcgaga cgcgcgccag ccgcctgcac
      301 ttcgaagggg aaaccccggc aaccatcgcc gacaccttcg ccaaggcgga aaagctcgac
      361 cgattggcga cgactacatc aggcgcgttg cgggcgacgc cctttgccat ggcctcgttg
      421 cttcagtaca tgcagcctgc gatcaacaag ggcgattggc tgccggctcc gctcaaaccg
      481 ctgaccccgc tcatttccgg agcgctgtcg ggcgccatgg accaggtggg caccaagatg
      541 atggaccgcg cgacgggtga tctgcattac ctgagcgcct cgccggacag gctccacgat
      601 gcgatggccg cttcggtgaa gcgccactcg ccaagccttg ctcgacaggt tctggacacg
      661 ggggttgcgg ttcagacgta ctcggcgcgc aacgccgtac gtaccgtatt ggctccggca
      721 ctggcgtcca gacccgccgt gcagggtgct gtggaccttg gtgtatcgat ggcgggtggt
      781 ctggctgcca acgcaggctt tggcaaccgc ctgctcagtg tgcagtcgcg tgatcaccag
      841 cgtggcggtg cattagtgct cggtttgaag gataaagagc ccaaggctca actgagcgaa
      901 gaaaacgact ggctcgaggc ttataaagca atcaaatcgg ccagctactc gggtgcggcg
      961 ctcaacgctg gcaagcggat ggccggtctg ccactggata tggcgaccga cgcaatgggt
     1021 gcggtaagaa gcctggtgtc agcgtccagc ctgacccaaa acggtctggc cctggcgggt
     1081 ggctttgcag gggtaggcaa gttgcaggag atggcgacga aaaatatcac cgacccggcg
     1141 accaaggccg cggtcagtca gttgaccaac ctggcaggtt cggcagccgt tttcgcaggc
     1201 tggaccacgg ccgcgctgac aaccgatccc gcggtgaaaa aagccgagtc gttcatacag
     1261 gacacggtga aatcgactgc atccagtacc acaggctacg tagccgacca gaccgtcaaa
     1321 ctggcgaaga ccgtcaaaga catgggcggg gaggcgatca cccataccgg cgccagcttg
     1381 cgcaatacgg tcaataacct gcgtcaacgc ccggctcgtg aagctgatat agaagagggg
     1441 ggcacggcgg cttctccaag tgaaataccg tttcggccta tgcggtcgaa gggtgggcgc
     1501 gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1561 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1621 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1681 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1741 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1801 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1861 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1921 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1981 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2041 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2101 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2161 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2221 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2281 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2341 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2401 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2461 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2521 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2581 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2641 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3001 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3061 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3121 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3181 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3241 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3301 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3361 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3421 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3481 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3541 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3601 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3661 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3721 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3781 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3841 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3901 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt
     3961 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata
     4021 atgccaactt tgtacaaaaa agcaggctcc gcggccgcc
//
