LOCUS       sequence-252547-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5537
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1451..1550)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1567..1586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1568..1586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1591..1607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1591..1607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1709..1728)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1720..2529
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1787..1806)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2676..3264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3165..3184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3418..3435
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3594..3621)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3713..3799)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3848..3870
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3862..3879
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3863..3879
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3895..3994
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgaatcccc tgcaacctat tcagcacagc
       61 attacaaatt cccaaatgag tggtggtcag caattagagg cggagggctc tcaggcccac
      121 aattcctatt cccatcctga caggatttcg ctttcccaat tgagccaaag cgctcaccta
      181 gctctagatc acctttcaac tcagcctaat accgatcacc aacgcgttgc atcactggta
      241 cgcaacgctg tgcaggacgg taagttccaa cttcaatcca gtaacgacac gcaagtaacc
      301 tataaaactt cagtctgtcc gccagctaac gccgacacca tgggggccgc ccacttaatt
      361 aataacgagc tgacggttca ggcccgatta aatgatcaac ttgagtacga catcgtcagc
      421 gctcatttgt atggcccttc ggaagccata tccatcgatg catccagtcc tccctcggcc
      481 aacgatctag cgtcctctgg cttgagcgaa cgtacgcatc taggtatgaa tcgtgtcctc
      541 ttacgctacg cggtgccccc tcgggaaacc gaagaccaat gtgttatggt gatcgacaaa
      601 atgccccccc ccaaacacgg caaaatgtct ttcttccgta ccactaatga cttgagcaaa
      661 ctgcctttgg gaatggagac gggcgggttg tccgacctga aattggctgg ttgtgaacgt
      721 atttcttccg tcgagcaggt gaagagtatc cgcgcagcgc ttggaggcgg gccgctcacc
      781 gtactagatc tgcgcgaaga atctcatgcg attgtcaacg gtttgcctat caccttacgt
      841 ggcccgatgg attgggccaa cgccggccta tcccaggttg acggagcggc acgtgaaagt
      901 gccatgatta cagaactgaa gcgcactaag tctttaacgt tggtcgatgc caattatgta
      961 aaaggtaaaa aaagtaatcc tcaaacgaca gaactgaaaa atttgaatgt ccggagcgag
     1021 cgagaagtcg ttacagaggc cggcgcgacc tatcgccgcg tggccattac cgaccataac
     1081 aggcctagtc cggaagcgac cgacgagcta gtagacatca tgcgccactg cctgcaggca
     1141 aatgagtcgc tagttgtgca ctgtaacggc ggtcggggcc gtactaccac ggctatgata
     1201 atggtcgaca tgcttaagaa cgctcgtaac cattccgcag aaaccctcat cacgcgtatg
     1261 gccaagctaa gctatgacta caacatgacg gatctaggca gcatttctgc actcaagcgg
     1321 ccattcctag aggacagact aaaatttctg caggcctttc acgactatgc ccgcaacaac
     1381 ccaagcggat tatctcttaa ttggacacag tggcgcgcaa aaatagcgtt agaaaagggt
     1441 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1501 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1561 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1621 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1681 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1741 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1801 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1861 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1921 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1981 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2041 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2101 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2161 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2221 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2281 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2341 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2401 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2461 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2521 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2581 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2641 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2701 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2761 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2821 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2881 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2941 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3001 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3061 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3121 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3181 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3241 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3301 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3361 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3421 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3481 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3541 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3601 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3661 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3721 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3781 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3841 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     3901 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     3961 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgcc
//
