LOCUS       sequence-252549-        3393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5328
FEATURES             Location/Qualifiers
     source          1..3393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(839..938)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(955..974)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(956..974)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(979..995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(979..995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1097..1116)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1108..1917
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1175..1194)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2064..2652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2553..2572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2806..2823
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2982..3009)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3101..3187)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3236..3258
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3250..3267
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3251..3267
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3283..3382
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgaatataa atcgacaact gcctgtatca
       61 ggctcggagc gattgttgac tcccgacgtg ggcgtatctc gccaggcttg ttccgaaagg
      121 cattattcta ctggacagga tcggcatgat ttttaccgtt ttgctgccag gctacatgtg
      181 gatgcgcagt gttttggtct gtcaatagac gatttgatgg ataagttttc tgacaagcac
      241 ttcagggctg agcatcctga atacagggat gtctatccgg aggaatgttc tgccatttat
      301 atgcataccg ctcaagacta ttctagtcac ctcgtaaggg gggaaatagg aacgccgctg
      361 taccgagagg tcaataatta tcttcgactt caacatgaga attctgggcg agaagctgaa
      421 attgataatc acgacgaaaa gctatcgcct cacataaaaa tgctttcatc tgcgcttaat
      481 cgtttaatgg atgtcgccgc ttttagagga acggtttata gaggcattcg cggtgattta
      541 gataccattg ctcggctcta ccatctattc gatacgggcg gccggtacgt agagcccgct
      601 ttcatgagta caactcgaat aaaggacagt gcccaggtgt ttgagccagg cacgccaaac
      661 aacatagctt tccagataag cctaaaaaga ggcgccgaca tttcgggatc ttcccaagcg
      721 ccctcagagg aagaaatcat gctacccatg atgagtgagt tcgtcattga acatgcatcc
      781 gctctttccg aaggaaagca tttatttgta ttaagtcaga ttaagggtgg gcgcgccgac
      841 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
      901 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
      961 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1021 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1081 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1141 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1201 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1261 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1321 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1381 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1441 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1501 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1561 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1621 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1681 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1741 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1801 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1861 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1921 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     1981 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2041 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2101 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2161 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2221 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2281 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2341 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2401 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2461 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2521 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2581 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2641 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2701 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2761 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2821 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2881 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2941 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3001 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3061 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3121 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3181 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3241 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
     3301 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
     3361 actttgtaca aaaaagcagg ctccgcggcc gcc
//
