LOCUS       sequence-252551-       11699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA E469delinsDK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-E469delinsDK
FEATURES             Location/Qualifiers
     source          1..11699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6659..6683)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6691..6709
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6734..6753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6735..6753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6759..6779)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6833..6874
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6895..7462
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6912..7464
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7056..7073)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7283..7302
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7476..8075
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7476..7495)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7972..7992
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8089..8677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8142..8162)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8560..8571)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8752..8985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9104..9122)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9190..9294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9295..9363,9364..10155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9513..9532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10326..10914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10815..10834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11068..11085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11325..11346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11361..11366,11367..11384,11385..11391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11399..11415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11404..11426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11423..11439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11423..11439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11489..11508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag
     3781 atcctcaatc gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt tgatatggtt
     3841 aaagatccag aagtacagga cttccgaaga aatattctga acgtttgtaa agaagctgtg
     3901 gatcttaggg acctcaattc acctcatagt agagcaatgt atgtctatcc tccaaatgta
     3961 gaatcttcac cagaattgcc aaagcacata tataataaat tagataaagg gcaaataata
     4021 gtggtgatct gggtaatagt ttctccaaat aatgacaagc agaagtatac tctgaaaatc
     4081 aaccatgact gtgtaccaga acaagtaatt gctgaagcaa tcaggaaaaa aactcgaagt
     4141 atgttgctat cctctgaaca actaaaactc tgtgttttag aatatcaggg caagtatatt
     4201 ttaaaagtgt gtggatgtga tgaatacttc ctagaaaaat atcctctgag tcagtataag
     4261 tatataagaa gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa
     4321 agcctttatt ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt
     4381 tccacagcta caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat
     4441 agtgcactca gaataaaaat tctttgtgca acctacgtga atgtaaatat tcgagacatt
     4501 gataagatct atgttcgaac aggtatctac catggaggag aacccttatg tgacaatgtg
     4561 aacactcaaa gagtaccttg ttccaatccc aggtggaatg aatggctgaa ttatgatata
     4621 tacattcctg atcttcctcg tgctgctcga ctttgccttt ccatttgctc tgttaaaggc
     4681 cgaaagggtg ctaaagagga acactgtcca ttggcatggg gaaatataaa cttgtttgat
     4741 tacacagaca ctctagtatc tggaaaaatg gctttgaatc tttggccagt acctcatgga
     4801 ttagaagatt tgctgaaccc tattggtgtt actggatcaa atccaaataa agataaaact
     4861 ccatgcttag agttggagtt tgactggttc agcagtgtgg taaagttccc agatatgtca
     4921 gtgattgaag agcatgccaa ttggtctgta tcccgagaag caggatttag ctattcccac
     4981 gcaggactga gtaacagact agctagagac aatgaattaa gggaaaatga caaagaacag
     5041 ctcaaagcaa tttctacacg agatcctctc tctgaaatca ctgagcagga gaaagatttt
     5101 ctatggagtc acagacacta ttgtgtaact atccccgaaa ttctacccaa attgcttctg
     5161 tctgttaaat ggaattctag agatgaagta gcccagatgt attgcttggt aaaagattgg
     5221 cctccaatca aacctgaaca ggctatggaa cttctggact gtaattaccc agatcctatg
     5281 gttcgaggtt ttgctgttcg gtgcttggaa aaatatttaa cagatgacaa actttctcag
     5341 tatttaattc agctagtaca ggtcctaaaa tatgaacaat atttggataa cttgcttgtg
     5401 agatttttac tgaagaaagc attgactaat caaaggattg ggcacttttt cttttggcat
     5461 ttaaaatctg agatgcacaa taaaacagtt agccagaggt ttggcctgct tttggagtcc
     5521 tattgtcgtg catgtgggat gtatttgaag cacctgaata ggcaagtcga ggcaatggaa
     5581 aagctcatta acttaactga cattctcaaa caggagaaga aggatgaaac acaaaaggta
     5641 cagatgaagt ttttagttga gcaaatgagg cgaccagatt tcatggatgc tctacagggc
     5701 tttctgtctc ctctaaaccc tgctcatcaa ctaggaaacc tcaggcttga agagtgtcga
     5761 attatgtcct ctgcaaaaag gccactgtgg ttgaattggg agaacccaga catcatgtca
     5821 gagttactgt ttcagaacaa tgagatcatc tttaaaaatg gggatgattt acggcaagat
     5881 atgctaacac ttcaaattat tcgtattatg gaaaatatct ggcaaaatca aggtcttgat
     5941 cttcgaatgt taccttatgg ttgtctgtca atcggtgact gtgtgggact tattgaggtg
     6001 gtgcgaaatt ctcacactat tatgcaaatt cagtgcaaag gcggcttgaa aggtgcactg
     6061 cagttcaaca gccacacact acatcagtgg ctcaaagaca agaacaaagg agaaatatat
     6121 gatgcagcca ttgacctgtt tacacgttca tgtgctggat actgtgtagc taccttcatt
     6181 ttgggaattg gagatcgtca caatagtaac atcatggtga aagacgatgg acaactgttt
     6241 catatagatt ttggacactt tttggatcac aagaagaaaa aatttggtta taaacgagaa
     6301 cgtgtgccat ttgttttgac acaggatttc ttaatagtga ttagtaaagg agcccaagaa
     6361 tgcacaaaga caagagaatt tgagaggttt caggagatgt gttacaaggc ttatctagct
     6421 attcgacagc atgccaatct cttcataaat cttttctcaa tgatgcttgg ctctggaatg
     6481 ccagaactac aatcttttga tgacattgca tacattcgaa agaccctagc cttagataaa
     6541 actgagcaag aggctttgga gtatttcatg aaacaaatga atgatgcaca tcatggtggc
     6601 tggacaacaa aaatggattg gatcttccac acaattaaac agcatgcatt gaactagaac
     6661 ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaaggg ttagttatga
     6721 tgaatattgt gtaccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt
     6781 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     6841 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc
     6901 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     6961 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     7021 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     7081 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     7141 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     7201 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     7261 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     7321 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     7381 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     7441 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc
     7501 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7561 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7621 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7681 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7741 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7801 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7861 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7921 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7981 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8041 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg
     8101 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8161 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8221 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8281 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8341 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8401 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8461 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8521 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8581 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8641 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     8701 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8761 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8821 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8881 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8941 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9001 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     9061 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     9121 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     9181 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9241 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9301 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9361 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9421 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9481 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     9541 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9601 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9661 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9721 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9781 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9841 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9901 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9961 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10021 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10081 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10141 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10201 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10261 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10321 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10381 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10441 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10501 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10561 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10621 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10681 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10741 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10801 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10861 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10921 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10981 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11041 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11101 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
    11161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    11221 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
    11281 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    11341 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    11401 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    11461 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11521 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    11581 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    11641 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
