LOCUS       sequence-252553-        3101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5052
FEATURES             Location/Qualifiers
     source          1..3101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..145)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    198..297
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1144..1243)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1355..1374)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1366..2175
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1433..1452)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2318..2906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2807..2826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3041..3068)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
       61 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      121 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
      181 gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
      241 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggcttcg
      301 aaggagatag aaccatgcac gcaaatcctt taagctcttt caacagagct caacatggca
      361 atctgactaa tgtagaggcc agccaagtta aatcggcagg aacctcttcc accactaata
      421 tagacagtaa aaacattgaa gaacatgttg cagacagact cagtgattta ggcagacctg
      481 atggtggatg gtttttcgag aagtcacttg gcaccttgaa aaatttaaat cttgagcagt
      541 tagccggaat ccatgatgta ctaaaattaa cagatggcgt aaagaacatt gtctcttttg
      601 gagctcggga aggaggcttc gagttggcaa tgcagtttcg tcatgattta tacagatctc
      661 aacatccgga tgaaaactcg ccgcacgatg ccgcaactca ttatcttgat gcaatcagcc
      721 tgcaatcaaa caaatttaca aaacttgaaa aactacaaca tgtagatgta tttaaaatgc
      781 aaaacccgtt ttgggatgtc gggtacaaaa acggaattgc gcacgcaaaa aaaatggcat
      841 tcttcataac gccagagtgg ctgggttctg atttctgtaa acaggaattc cagtggctta
      901 gcgaaacaaa aaacaaagac ataaaatctg catttgtgat ctttaaagat gtagacttaa
      961 aaagcaaaaa tatgacaagt atcttcaatt ttgcagactt ccataaatca cgcgtcatga
     1021 tggcaagcac acctcccgaa tcgggattga ataatgtaaa aatcgaaaat agcgttgacc
     1081 tgaatttcaa gaggttatta actgaccgtg agtcatggga actaaataat ttcctaggcg
     1141 actacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1201 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagctc
     1261 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1321 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1381 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1441 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1501 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1561 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1621 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1681 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1741 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1801 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1861 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1921 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1981 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2041 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2101 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2161 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2221 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagttt tcgttccact
     2281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3001 accgtattac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     3061 ggccttctgc ttagtttgat gcctggcagt ttatggcggg c
//
