LOCUS       sequence-252559-        4062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5108
FEATURES             Location/Qualifiers
     source          1..4062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1619..1718)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1735..1754)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1736..1754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1759..1775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1759..1775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1877..1896)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1888..2697
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1955..1974)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2844..3432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3333..3352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3586..3603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3762..3789)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3881..3967)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4016..4038
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4030..4047
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4031..4047
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgcacatc aaccaatccg cccaacaacc gcctggcgtt
      181 gcaatggaga gttttcggac agcttccgac gcgtcccttg cttcgagttc tgtgcggtct
      241 gtcagcacta cctcgtgccg cgatctacaa gctattaccg attatctgaa acatcacgtg
      301 ttcgctgcgc acaggttttc ggtaataggc tcaccggatg agcgtgatgc cgctcttgca
      361 cacaacgagc agatcgatgc gttggtagag acacgcgcca accgcctgta ctccgaaggg
      421 gagacccccg caaccatcgc cgaaacattc gccaaggcgg aaaagttcga ccgtttggcg
      481 acgaccgcat caagtgcttt tgagaacacg ccatttgccg ctgcctcggt gcttcagtac
      541 atgcagcctg cgatcaacaa gggcgattgg ctagcaacgc cgctcaagcc gctgaccccg
      601 ctcatttccg gagcgctgtc gggagccatg gaccaggtgg gcaccaaaat gatggatcgt
      661 gcgaggggtg atctgcatta cctgagcact tcgccggaca agttgcatga tgcgatggcc
      721 gtatcggtga agcgccactc gcctgcgctt ggtcgacagg ttgtggacat ggggattgca
      781 gtgcagacgt tctcggcgct aaatgtggtg cgtaccgtat tggctccagc actagcgtcc
      841 agaccgtcgg tgcagggtgc tgttgatttt ggcgtatcta cggcgggtgg cttggttgcg
      901 aatgcaggct ttggcgaccg catgctcagt gtgcaatcgc gcgatcaact gcgtgggggg
      961 gcattcgtac ttggcatgaa agataaagag cccaaggccg cgttgagtga agaaactgat
     1021 tggcttgatg cttacaaagc gatcaagtcg gccagctact caggtgcggc gctcaatgcg
     1081 ggcaagcgga tggccggcct gccactggac gtcgcgaccg acgggctcaa ggcggtgaga
     1141 agtctggtgt cggccaccag cctgacaaaa aatggcctgg ccctagccgg tggttacgcc
     1201 ggggtaagta agttgcagaa aatggcgacg aaaaatatca ctgattcggc gaccaaggct
     1261 gcggttagtc agctgagcaa cctggtgggt tcggtaggcg ttttcgcagg ctggaccacc
     1321 gctggactgg cgactgaccc tgcggttaag aaagccgagt cgtttataca ggataaggtg
     1381 aaatcgaccg catctagtac cacaagctat gttgccgacc agaccgtcaa actggcgaaa
     1441 acagtcaagg acatgagcgg ggaggcgatc tccagcaccg gtgccagctt acgcagtact
     1501 gtcaataacc tgcgtcatcg ctccgctccg gaagctgata tcgaagaagg tgggatttcg
     1561 gcgttttctc gaagtgaaac accgtttcag ctcaggcgtt tgaagggtgg gcgcgccgac
     1621 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1681 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1741 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1801 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1861 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1921 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1981 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2041 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2101 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2161 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2221 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2281 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2341 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2401 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2461 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2521 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2581 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2641 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2701 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2761 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3481 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3541 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3601 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3661 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3721 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3781 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3841 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3901 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3961 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     4021 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
