LOCUS       sequence-252562-        3615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5099
FEATURES             Location/Qualifiers
     source          1..3615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1061..1160)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1177..1196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1178..1196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1201..1217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1201..1217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1319..1338)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1330..2139
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1397..1416)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2286..2874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2775..2794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3028..3045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3204..3231)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3323..3409)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3458..3480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3472..3489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3473..3489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3505..3604
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgaatcgca tttcaaccag ctcagtaaat
       61 tccagcttca attacacggc ccctacggag gaagcgcaaa accgcttcgc ctcagcgccc
      121 gacaattccc ctctagttgt caccacaaca tctatcgccc aagcgtcgga agggctacaa
      181 aggccggggg caacgctaag catgcaggcc cagcgactgc gccaattgat ggggagcccg
      241 tctgagcagt gccggaggga cacaatgtta gctaaagctt ttgatgctca acgcctaaac
      301 attaacactc aagcaggctc ttccaacagc ccacacttga acgctctcaa cacgctccaa
      361 caacgacact tcaaacctgc ggctggtggg ctagaaatcc cagttacatc caactcctta
      421 ttgggcggtg gcaggcaagt ctatcaaatt ggctcatcgt cacgcgagct aagccaccga
      481 ccggtcaatg atcaggaccg cgcgcccttc agggcgcttg agcggctgca cgccgagttg
      541 tttagaggtg ggccgattga gtttgtgcct agaggcagca acgtgttggc ctcaaacgtg
      601 agggatgtcg acatggacga gttcgatgtc atcaactcta aagacggctg ccaaggcatt
      661 ggcaccactg gcctgggacc ctgcattgca gtgtgtgcaa gaggcatgga tagagaaggg
      721 cttccggtgc tgggtgtcta tcaccacagt ggtatcggct caccagagga taccatggct
      781 actcttgatc aagcgatgcg cgataaaggt gctttgcaaa tcaaatactc cctggtaggc
      841 ggcatgatca tgcctaaaga ggaagaggct ggcagctatg acgacgagca aagctttttg
      901 gcattgaaag gcagttattc aatcgaaggg gcgcgcttgc atgtatccga aggcgaagag
      961 gacgtgcata ccggcgagga caacagtgtc aatgttctgc tgatgcctga ccgcgttctg
     1021 tacggtcgcg acacgctcta ctgcaagggt gggcgcgccg acccagcttt cttgtacaaa
     1081 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1141 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1201 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1261 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1321 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1381 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1441 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1501 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1561 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1621 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1681 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1741 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1801 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1861 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1921 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     1981 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2041 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2101 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2161 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2221 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2401 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2641 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2881 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2941 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3001 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3061 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3121 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3181 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3241 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3301 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3361 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3421 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     3481 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     3541 tcgttgcaac aaattgatga gcaatgcttt cttataatgc caactttgta caaaaaagca
     3601 ggctccgcgg ccgcc
//
