LOCUS       sequence-252564-        3462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP3417
FEATURES             Location/Qualifiers
     source          1..3462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(908..1007)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1024..1043)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1025..1043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1048..1064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1048..1064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1166..1185)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1177..1986
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1244..1263)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2133..2721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2622..2641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2875..2892
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3051..3078)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3170..3256)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3305..3327
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3319..3336
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3320..3336
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3352..3451
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgaacatta cgccgctcac gtcagccgcg
       61 ggcaagggct cgtccgcaca aggcacagac aaaatttcca ttcccaactc cacgcgcatg
      121 atcaatgccg cttcaatcaa gtggttgaat aaggtgcgta gcgccatcag tgaccacatc
      181 cgcaccagca tcgagaaagg gaaactgttc gagctcgcct ccttgggcag caacatgttc
      241 ggtgtcccgg ctctttcagc gcgcccctcg acgctccaac ctgtgttggc gtttgaggct
      301 gaccccaatc acgacctgaa ccttgtcagg gtctatatgc aggacagcgc cggcaagctc
      361 actccctggg acccgacgcc caacgcggtc acgacgacgt cgaatccatc agagcctgat
      421 gcgcagagcg atacggcttc gtcatcatta cctcggcggc ctcccgcagg ctcggtgctg
      481 agtttgctgg gcattgcgct ggatcacgcg caacgccaca gtcctcgcgc ggacaggtct
      541 gccaagggac gacctggccg agaggagagg aacggggcaa ggttcaatgc caagcaaaca
      601 aagccgacag aggctgaagc ctacggtgat catcagacac ccaatcctga tttgcacagg
      661 caaaaagaga cagctcaacg cgttgctgaa agcatcaaca gcatgcgaga gcagcaaaat
      721 ggaatgcaac gcgccgaagg gcttctcaga gccaaagaag cgttgcaagc tcgggaagcc
      781 gcgcgcaagc agcttctgga cgtgctcgag gccatccagg ctggccgtga agactccacc
      841 gacaagaaga tcagcgccac tgaaaagaac gccacgggca tcaactacca gaagggtggg
      901 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      961 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1021 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1081 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1141 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1201 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1261 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1321 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1381 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1441 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1501 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1561 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1621 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1681 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     1741 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1801 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1861 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     1921 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     1981 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2041 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2101 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2161 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2221 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2281 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2341 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2401 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2461 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2521 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2581 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2641 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2701 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2761 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2821 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2881 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2941 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3001 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3061 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3121 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3181 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3241 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3301 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg
     3361 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     3421 ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg cc
//
