LOCUS       sequence-252566-        4083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5238
FEATURES             Location/Qualifiers
     source          1..4083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1508..1607)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1624..1643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1625..1643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1648..1664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1648..1664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1766..1785)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1777..2586
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1844..1863)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2733..3321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3222..3241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3475..3492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3651..3678)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3770..3856)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3905..3927
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3919..3936
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3920..3936
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3952..4051
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             4063..4083
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cccttcacca tgcaaataaa gaacagtcat ctctattcag cttcaagaat ggtgcagaat
       61 acttttaatg cctcgcctaa gatggaagta actaatgcaa tagcaaaaaa taatgaacct
      121 gctgcgctga gcgctacgca aactgcaaag acacacgaag gcgattcaaa aggccaatcc
      181 agcaataact ctaaattgcc cttccgcgcc atgaggtacg ctgcatacct tgcaggcagc
      241 gcctacctct acgataaaac tgccaataat ttttttcttt ctaccacttc tctgcatgat
      301 ggcaaaggtg gttttaccag cgatgccagg cttaacgatg cacaagataa agcgcgaaag
      361 cgctaccaaa acaaccatag cagcactctt gaaaataaaa actcgctttt aagcccgctt
      421 aggctttgcg gagagaatca gttcttaacg atgattgatt atcgtgcagc aactaagatt
      481 tacctctccg acctagttga cacggagcaa gcgcacacat caattctgaa gaatattatg
      541 tgcctgaaag gtgagcttac caatgaagag gcaataaaaa aactcaaccc ggaaaaaaca
      601 ccaaaagact atgaccttac aaatagcgaa gcctatataa gcaagaacaa atattctttg
      661 accggcgtta aaaatgagga gacgggatct actggttata catctcgttc tatcacaaag
      721 ccatttgtgg aaaaaggcct gaaacacttt ataaaagcga ctcatggcga aaaagctctc
      781 acgcccaagc agtgtatgga aactcttgat aacttacttc gaaaaagtat cacgctcaac
      841 agtgattccc aattcgcagc aggccaggca cttttggttt tcagacaggt ctatgcgggt
      901 gaagacgctt ggggggatgc ggaacgggtc atattgaaaa gccattataa tcggggcact
      961 gtactccaag atgaagctga taaaatagaa ctaagtaggc cgttctcaga gcaagattta
     1021 gcaaagaaca tgtttaagag gaataccagc attgcagggc cagtgctcta ccacgcatat
     1081 atttatatac aagaaaaaat cttcaagcta ccccccgaca aaatagaaga tttgaaacat
     1141 aaatcaatgg cagacttgaa aaacctgcct ttgactcatg ttaagcttag caattccggt
     1201 gtgggatttg aagacgcctc agggttagga gactcgttta cagctctcaa cgcgacgtcc
     1261 tgtgttaatc acgcaagaat aatgagtggt gagcctccct tgtcaaaaga tgatgttgtg
     1321 attctgatag gttgcctcaa cgccgtatac gacaattcga gcggaataag gcattctctc
     1381 cgcgaaattg cacgagggtg ctttgtgggt gctggtttta cggtccagga cggtgacgac
     1441 ttctacaaac agatctgcaa aaacgcctct aagcagtttt acaacggcta caagggtggg
     1501 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1561 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1621 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1681 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1741 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1801 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1861 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1921 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1981 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2041 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2101 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2161 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2221 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2281 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2341 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2401 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2461 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2521 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2581 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2641 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2701 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2761 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2821 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2881 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2941 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3001 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3061 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3121 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3181 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3241 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3301 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3361 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3421 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3481 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3541 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3601 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3661 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3721 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3781 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3841 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3901 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg
     3961 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     4021 ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg ccttgtttaa ctttaagaag
     4081 gag
//
