LOCUS       sequence-252569-        3942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP3373
FEATURES             Location/Qualifiers
     source          1..3942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1388..1487)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1504..1523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1505..1523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1528..1544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1528..1544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1646..1665)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1657..2466
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1724..1743)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2613..3201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3102..3121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3355..3372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3531..3558)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3650..3736)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3785..3807
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3799..3816
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3800..3816
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3832..3931
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgcatcgtc ctatcaccgc aggccatacc
       61 acctcacgtc tcatcctaga tcagtcaaaa caaatatcac gtaccccatc ggaaagtagc
      121 gcgcaatcag cgctttctca gcaagcaagc atgagcagcc cagttttgga gcggtcgaaa
      181 agtgcgccag ctttattgac tgcggcacag cgcacgatgc ttgcacaagt gggagcctgt
      241 aacgctcatc tgacctcaga tgaaaacatg gccatcaacg aactgagatc acacaagccc
      301 cttttaccta aggatacgtg gtttttcacc gatcctaaca aggacccaga tgatgtcgtg
      361 acctacacct tgggcaagca attgcaggct gagggctttg tgcacatcac ggatgtagtg
      421 gcgacactgg gtgatgctga agttcgctct caacgtgccg agatggccaa aggcgtgttc
      481 aacaagcttg agttgcatga cgtgcatgtg tcgcgtggtc gggattacgc aatgaattcg
      541 cttcagtcga aggaacatgc caaattttta ctggaaggtc atgctttaag ggctggacct
      601 ggtgaaatac accgcgacag cttgcaggac atgagcaggc gcctggcccg tgcgccacat
      661 ggagtcggta ttgtcgtaat tgcaggcatg agtgatatca atgcgctcat cactacctgc
      721 ccggatatgg tgcgcgaacg ggttgatgac atcaccatca tgggcggcgt cgagccttta
      781 aaggacgcag atggttttgt acagcctgat gcacgcgctt acaacaatgc gaccgacatg
      841 gacgctgcgc gcagtcttta tcggaaagcg caggagcttg gcattccact tcgtatagtg
      901 acaaaggagg cggcctataa aacggcggtt tcgccttcat tttacgaagg gatagcgggg
      961 agcggacatc cagtaggcca ctacctgaga gacgttcaga agagtgcgtt gaaaggcctc
     1021 tgggaaggta ttcaagctgg attgcttccc gggttggatg actcatggtt ctttcggacg
     1081 ttcatgccga atgcacagat tgaagcagca caactggata aaaataaaga gagttcgttt
     1141 gaagatatct ggcctaaggt gacgaagcta aacctgtatg atcctctgac attactggcc
     1201 tcagtgccag gggcggcaaa actgctattt aaaccaaaag ctatacacac agaaggattt
     1261 ggtgttgtag agcaagtagg tccagatgat gtgacgcatc cagagaaagc aaagttattg
     1321 atgtccgctt tagccaaatc tgcgcttgtc cagtcgacgg tagccccaga taagggtggg
     1381 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1441 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1501 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1561 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1621 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1681 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1741 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1801 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1861 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1921 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1981 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2041 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2101 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2161 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2221 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2281 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2341 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2401 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2461 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2521 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3481 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3541 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3601 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3661 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3721 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3781 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg
     3841 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     3901 ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg cc
//
