LOCUS       sequence-252572-       11690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA E103_P104delEP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-E103_P104delEP
FEATURES             Location/Qualifiers
     source          1..11690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6650..6674)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6682..6700
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6725..6744)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6726..6744)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6750..6770)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6824..6865
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6886..7453
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6903..7455
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7047..7064)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7274..7293
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7467..8066
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7467..7486)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7963..7983
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8080..8668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8133..8153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8551..8562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8743..8976
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9095..9113)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9181..9285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9286..9354,9355..10146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9504..9523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10317..10905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10806..10825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11059..11076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11316..11337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11352..11357,11358..11375,11376..11382)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11390..11406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11395..11417
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11414..11430
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11414..11430
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11480..11499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgtaggca accgtgaaga aaagatcctc
     3781 aatcgagaaa ttggttttgc tatcggcatg ccagtgtgtg aatttgatat ggttaaagat
     3841 ccagaagtac aggacttccg aagaaatatt ctgaacgttt gtaaagaagc tgtggatctt
     3901 agggacctca attcacctca tagtagagca atgtatgtct atcctccaaa tgtagaatct
     3961 tcaccagaat tgccaaagca catatataat aaattagata aagggcaaat aatagtggtg
     4021 atctgggtaa tagtttctcc aaataatgac aagcagaagt atactctgaa aatcaaccat
     4081 gactgtgtac cagaacaagt aattgctgaa gcaatcagga aaaaaactcg aagtatgttg
     4141 ctatcctctg aacaactaaa actctgtgtt ttagaatatc agggcaagta tattttaaaa
     4201 gtgtgtggat gtgatgaata cttcctagaa aaatatcctc tgagtcagta taagtatata
     4261 agaagctgta taatgcttgg gaggatgccc aatttgatgt tgatggctaa agaaagcctt
     4321 tattctcaac tgccaatgga ctgttttaca atgccatctt attccagacg catttccaca
     4381 gctacaccat atatgaatgg agaaacatct acaaaatccc tttgggttat aaatagtgca
     4441 ctcagaataa aaattctttg tgcaacctac gtgaatgtaa atattcgaga cattgataag
     4501 atctatgttc gaacaggtat ctaccatgga ggagaaccct tatgtgacaa tgtgaacact
     4561 caaagagtac cttgttccaa tcccaggtgg aatgaatggc tgaattatga tatatacatt
     4621 cctgatcttc ctcgtgctgc tcgactttgc ctttccattt gctctgttaa aggccgaaag
     4681 ggtgctaaag aggaacactg tccattggca tggggaaata taaacttgtt tgattacaca
     4741 gacactctag tatctggaaa aatggctttg aatctttggc cagtacctca tggattagaa
     4801 gatttgctga accctattgg tgttactgga tcaaatccaa ataaagaaac tccatgctta
     4861 gagttggagt ttgactggtt cagcagtgtg gtaaagttcc cagatatgtc agtgattgaa
     4921 gagcatgcca attggtctgt atcccgagaa gcaggattta gctattccca cgcaggactg
     4981 agtaacagac tagctagaga caatgaatta agggaaaatg acaaagaaca gctcaaagca
     5041 atttctacac gagatcctct ctctgaaatc actgagcagg agaaagattt tctatggagt
     5101 cacagacact attgtgtaac tatccccgaa attctaccca aattgcttct gtctgttaaa
     5161 tggaattcta gagatgaagt agcccagatg tattgcttgg taaaagattg gcctccaatc
     5221 aaacctgaac aggctatgga acttctggac tgtaattacc cagatcctat ggttcgaggt
     5281 tttgctgttc ggtgcttgga aaaatattta acagatgaca aactttctca gtatttaatt
     5341 cagctagtac aggtcctaaa atatgaacaa tatttggata acttgcttgt gagattttta
     5401 ctgaagaaag cattgactaa tcaaaggatt gggcactttt tcttttggca tttaaaatct
     5461 gagatgcaca ataaaacagt tagccagagg tttggcctgc ttttggagtc ctattgtcgt
     5521 gcatgtggga tgtatttgaa gcacctgaat aggcaagtcg aggcaatgga aaagctcatt
     5581 aacttaactg acattctcaa acaggagaag aaggatgaaa cacaaaaggt acagatgaag
     5641 tttttagttg agcaaatgag gcgaccagat ttcatggatg ctctacaggg ctttctgtct
     5701 cctctaaacc ctgctcatca actaggaaac ctcaggcttg aagagtgtcg aattatgtcc
     5761 tctgcaaaaa ggccactgtg gttgaattgg gagaacccag acatcatgtc agagttactg
     5821 tttcagaaca atgagatcat ctttaaaaat ggggatgatt tacggcaaga tatgctaaca
     5881 cttcaaatta ttcgtattat ggaaaatatc tggcaaaatc aaggtcttga tcttcgaatg
     5941 ttaccttatg gttgtctgtc aatcggtgac tgtgtgggac ttattgaggt ggtgcgaaat
     6001 tctcacacta ttatgcaaat tcagtgcaaa ggcggcttga aaggtgcact gcagttcaac
     6061 agccacacac tacatcagtg gctcaaagac aagaacaaag gagaaatata tgatgcagcc
     6121 attgacctgt ttacacgttc atgtgctgga tactgtgtag ctaccttcat tttgggaatt
     6181 ggagatcgtc acaatagtaa catcatggtg aaagacgatg gacaactgtt tcatatagat
     6241 tttggacact ttttggatca caagaagaaa aaatttggtt ataaacgaga acgtgtgcca
     6301 tttgttttga cacaggattt cttaatagtg attagtaaag gagcccaaga atgcacaaag
     6361 acaagagaat ttgagaggtt tcaggagatg tgttacaagg cttatctagc tattcgacag
     6421 catgccaatc tcttcataaa tcttttctca atgatgcttg gctctggaat gccagaacta
     6481 caatcttttg atgacattgc atacattcga aagaccctag ccttagataa aactgagcaa
     6541 gaggctttgg agtatttcat gaaacaaatg aatgatgcac atcatggtgg ctggacaaca
     6601 aaaatggatt ggatcttcca cacaattaaa cagcatgcat tgaactagaa cccagctttc
     6661 ttgtacaaag tggtgatatc caattaaccc tcactaaagg atgatgtgtt tgatttgaat
     6721 tgaaccctat agtgagtcgt attaccaccc aacttttcta tacaaagtgg ttgatatcca
     6781 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     6841 ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc
     6901 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     6961 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     7021 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     7081 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     7141 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     7201 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     7261 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     7321 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     7381 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     7441 aacacgatga taatatggcc acacatatga ccgagtacaa gcccacggtg cgcctcgcca
     7501 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     7561 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     7621 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7681 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7741 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7801 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     7861 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7921 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7981 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     8041 gcatgacccg caagcccggt gcctaaatcg atagatccta atcaacctct ggattacaaa
     8101 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8161 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8221 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8281 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8341 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8401 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8461 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8521 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8581 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8641 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     8701 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8761 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9001 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     9061 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     9121 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     9181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9301 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9361 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9421 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9481 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     9541 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9601 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9661 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9721 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9781 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9841 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9901 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9961 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10021 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10081 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10141 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10981 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11041 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    11101 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
    11161 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    11221 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt
    11281 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    11341 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    11401 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt
    11461 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    11521 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    11581 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    11641 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
