LOCUS       sequence-252577-        9479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of T. thermophilus Csm in E. coli (Csm1-5).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Liu TY, Liu JJ, Aditham AJ, Nogales E, Doudna JA
  TITLE     Target preference of Type III-A CRISPR-Cas complexes at the 
            transcription bubble.
  JOURNAL   Nat Commun. 2019 Jul 5;10(1):3001. doi: 10.1038/s41467-019-10780-2.
  PUBMED    31278272
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-5"; volume: "10(1):3001. doi"; pages: " 
            10.1038/s41467-019-10780-2"
COMMENT     Sequence Label: pCDF-5xT7-TtCsm
FEATURES             Location/Qualifiers
     source          1..9479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2504..2522
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2523..2547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2562..2584
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        3002..3020
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3021..3045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3060..3082
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        3821..3839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3840..3864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3879..3901
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        4790..4808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4809..4833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4848..4870
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6074..6097
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6098..6127
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(6146..6164)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6160..6207
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6378..7169)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7170..7261)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7309..8047)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(8257..9339)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9301..9320
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9340..9417)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(9463..9479,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggcaagca tgctgggtga tggtctgagc gttgcactgg caggtctgct
      121 gcatgatgtt ggtaaactgt atagccgtgc acgttggggt gaacgtgatg atcaggttcc
      181 ggatcgtacc cataccgcat ataccgcaca ttttgttcgt gaacatgcag gtctgtttcg
      241 tggtgcaggt ctggatccgg attggctggc acgtaccgca agccgtcatc atgaaggttg
      301 gcgtgatcgt ccgcagtatc gtccggaaac accggaagaa tggtgtgttg cactggccga
      361 tacctatgca agcaaagaac gtgaagaggg tgaaggtggt ggtagccctc cggaagttcc
      421 gctgagccct ccgtttcgtc gtctgctgct gggtggtgaa gaaggtcgcg aaggcggtta
      481 tagtccggtt ggtgccggtg gtcgtgttgg tctggaagcc ggtggtctgt atccggaaga
      541 acgtccgaat gttagcaaag atgtttataa acgtctgctg gaacgtctgg aaggtcgtct
      601 ggaagaaatg gcacgtttta gcctgggtaa agaagcactg gttctgaatc tggcactggc
      661 actgcaagaa accctgagcc tggttccgag cgatacccag agcgaaccgg atgttagcct
      721 gtatgatcat ctgcgtctga ccgcagccat tgcacatgca ctgtggctgt ttcatggtgg
      781 tagtccgagc gcacagaatc tgcgtcagga tggtgaaaaa tttctgctgg ttgttggtga
      841 tatgggtggt attcagggtc atatttatcg tattgccggt gccgaagccg gtgttggtgg
      901 tattgcaaaa cgtctgcgtg cacgtagcct ggaagttagc ctggcagcag aagcaatggc
      961 actgggtctg ctgtggcgtc tgggtctgac cagcctgaat cgtattctgg gtgcgggtgg
     1021 taaattctat ctgctgctgc cgaataccga agaagcacgc gcagcactgg aaggcacccg
     1081 tgaagcatgg ggtcgttggg cactgaaacg tggtggtagt ctggttccgc atctggcatg
     1141 ggttgcattt cgtggtcagg attttcgtga ttttgcagca ctgctgaaac gtctgcatga
     1201 agcactggca cgtgaaaaac tgcgtccgtt tgcatttctg gcaagcaccg gtggtgttct
     1261 gggtgcaccg ctgcgtccgt gtgcagcatg tggtctggaa ccggcacgtc gtgatgaacc
     1321 gggtagcctg tgtccggatt gtgaacgtga agcagcactg ggtgcacgtc tgcctcgtag
     1381 cgatcgtgtt ggtttttttc tggaagaagc accgcgtccg tatctggatt ttccgggtct
     1441 gaaagttggt ctgggtggtc cgctggaagg tgcatttcac ctgtttcgtg cacgtctgga
     1501 ttttgcaccg tggcctgatc cgagcgaagc aaaaccgctg ctgggtcatc tgcctcgtgt
     1561 tgaacatgca ctgaaagcaa aaggttggag cctggaagca tatcgtgcat gggcagaaga
     1621 agagggtctg ctggaagatg aagaaccgca tccggaaaaa gtgctgacct ttgcagaact
     1681 ggcagcactg agcgaaggtg caccgtatct gggtgcactg atgctggatg cagatcgtat
     1741 gggtgaagca tttgcaaccg gttttcgtcg tgaaggtcgt gatctggcaa ccccgagccg
     1801 tctggcagcc ctgagccgta ccctggaagt gttttttacc accgaagttc tgaccctgct
     1861 ggaagaaccg cgtcgttatc gtgaacgtct gggttgggat gatctggaag cacagcgtaa
     1921 agaagcccgt tatccgctgc tgtatagcgt ttatagcggt ggtgatgacc tgtttctgct
     1981 gggtccgtgg gatgcactgc tggattttgc cctggatctg gaacgcctgt atcgtctgtt
     2041 tacccgtcat ccgcgtctga cactgagcgg tggttttctg ctggtgcctc cgagcctgcc
     2101 ggtgccggaa ctggctcgtc tgctgggtga agcagaaaaa cgtgcaaaag ccgaaggtcg
     2161 tgaacgcctg tttctgtttg gtcaggcagt tccgtgggaa accctgcgtg gtctgcgtgc
     2221 ctgggctgaa ggtctgcgcc aggatctgcg tgcagaacgt gttagccgtg cacaggttta
     2281 tcgttggctg ctgctgtggc gtcgttttag tccgctggaa gatccgggtg aacgtatgcg
     2341 ttataaacca ctgctggcat atgcactgcg tcgtgtgcgc gaacgtgatg aagaagcatg
     2401 gaaacgttat ctgaaactgc tggatcatca ggatccggca tgggcatatc tgccggtttg
     2461 ggttcagtgg gcactgtatc gcgaacgtcg tgtttaactc gagtaatacg actcactata
     2521 ggggaattgt gagcggataa caattccgac gtcgaaataa ttttgtttaa ctttaagaag
     2581 gagatatacc atgcctgcac tggaattctt caaagataaa gaacgtggtg ttctggatcc
     2641 gaaagttttt gaacgtgccc gtgaagttgc cgaaggtctg gcacgtggta aactgaaaag
     2701 cagccagttt cgtaactatt ttgcagaact gcgtgccctg gaaaatcgtt ttgaacgcga
     2761 acgtcgtaaa gaaggtgaag aactggcatt tgcacgtctg gttccgcagc tggaactgct
     2821 gcgtgcaaaa ctgttttata acacccgtag ccagggtccg ctgcgtgatg caaaagaatt
     2881 tgttgaattt atggaagaag cactggaagc aggtaaacgt agcccgaaag attttgaagc
     2941 catgatgaaa tatgttgaag cagtgctggc atatttctat gcactggcaa aataagtcga
     3001 ctaatacgac tcactatagg ggaattgtga gcggataaca attccgacgt cgaaataatt
     3061 ttgtttaact ttaagaagga gatataccat gaaactgaaa aaagtgattc gcattcgtag
     3121 cgttctgctg gcaaaaaccg gtctgcgtat tggtatgagc cgtgatcaga tggccattgg
     3181 tgatctggat aatccggttg ttcgtaatcc gctgaccgat gaaccgtata ttccgggtag
     3241 cagcctgaaa ggtaaactgc gttatctgct ggaatggtca ctgggtggtg attacattct
     3301 gaaagcaaaa gaacgtcagg tttatgcaag tccggatccg aaagatccgg ttgcacgtat
     3361 ttttggtctg gcaccggaaa atgatgaacg tagcctggca gttgcacgtg aacgtggtcc
     3421 gacccgtctg ctggttcgtg atgcatatct gaccgaagat gcaaaagaag cactggaacg
     3481 taccagcgca cgtggtggtc tgtataccga aattaaacaa gaagtgttta ttccgcgtct
     3541 gggtggtaat gcaaatccgc gtaccaccga acgtgttccg gcaggcgcac gttttcgtgt
     3601 tgaaatgacc tatcgtgtgc tggatgatct ggacgaagaa tattttggta aatatctgct
     3661 gcgtgccctg gaactgctgg aactggatgg cctgggtggt catattagcc gtggttatgg
     3721 tcaggtgtat tttctgcatc cggaacgtct gacagaagat caagaaggtt ggcctctgaa
     3781 agaacgcctg aaagttgaag aagttgtgct gtaacaattg taatacgact cactataggg
     3841 gaattgtgag cggataacaa ttccgacgtc gaaataattt tgtttaactt taagaaggag
     3901 atataccatg cgtgcaaccc tgtttcgtct gtattttcag ggtccgctga aagcactgcc
     3961 tcgtgcaccg accctgctgg gtcacctgtt ttggtggtat cgttataccc atggtcgtga
     4021 agcactggaa gaactgctgg aacgttttcg tcaagaaccg ccttttcgtc tgagcagcgt
     4081 ttatccggaa ggttggctgc ctcgtccgaa actgcctccg gttcaggttg aagaaaccac
     4141 cctgcgtaaa gcactgaaaa gcctgagcct ggttcgtctg gatacctttc aggcactggc
     4201 agaacgtggt gaagaagccc tgctggaagc accggaagtt cagggtaaag cacgtccgcc
     4261 tgaaatgcgt cgtctgcgtc gtacccgtgt tggtgttgat cgtgcagcag gcaccgcacg
     4321 tccgggtgtt ctgtttaccc aagaatacct gtttccggat ccgcgtaccc cgtatgcact
     4381 gtatgttctg ggtgaagcac cgtttgatct gggcgaagcc ctggcatttg ttggtgaaat
     4441 gggttatggt ggtcaggcaa gcctgggtct gggtcgtttt cgtgttgaag gtccgtttgc
     4501 agttgaactg ccggaagcaa aagaaccgaa tgcatatgcc accctggcac cgggtcctct
     4561 ggaagaggca ctgtattatg aagttgaacc gtattggggt cgtctgggtg gtgcatatgt
     4621 tggtgcacgt ccttttaaac gtccgtatct gcgtgcaaaa gaaggttcag tttatcgcgg
     4681 tccgacccat cgtctgctgg aagttacccc gaccgaaccg cctgaagccg gtgcacgtgt
     4741 ttgggaagcg ctggttgttt ttccgctggg tgttcgtgtg taaaagcttt aatacgactc
     4801 actatagggg aattgtgagc ggataacaat tccgacgtcg aaataatttt gtttaacttt
     4861 aagaaggaga tataccatgg gtggtccgcg tggtctgagc ctgggtggtg aaggtatgcg
     4921 ctttctgcgt agctttcgtc tggaactgga agccctgagt ccggttcatg ttggcaccgg
     4981 tgaagcatat cctgcatatg cctatgttcc ggattttgca cgtaaagcag ttcatctgct
     5041 ggatccggca gcactgctgc tggcactgcc ggaagcacgt cgtcgtcagt atctggaaaa
     5101 agttgcacag ggtccgaaag cagcacaaga agttctgcgt tacctgctgg aagaggatca
     5161 gctgcctcgt gaagcagttc tgcataccct gcctgcaagc aaagcatttc tggaagcgct
     5221 gcgtagcgca accgaagagg cactgctgga atatcgtccg ctgccacgta gccctctggg
     5281 tgcatatctg cctggtagca gcgttaaagg tgcactgcgt accgcatggc tgtttcatgt
     5341 tctggttcgt gaaggtaaag ttgccgtttt tgatcgtaaa gaaggtgttt ggcgtctgcg
     5401 tgcctggcgt gaaggtgatg gtggcaccca tgtttatccg agccgtaatc cgagcctgta
     5461 tgaaaatcag gcatttgaag gtgcagttct gggttatgca cgtgaaggta aaggtcgtcg
     5521 tatgagcctg gatctgtatc gtgatccgtt tcgtgcagtt cgtctgagcg atagcggtcc
     5581 ggttgaaacc tttctgaatc gtctgggtgt ttttcatccg cataaagata ccagccgtat
     5641 ggttctgctg gcagaaacat ttcgtattgg cacccgtttt gcactgaccc tgcgttatca
     5701 tgaaggtctg agccgtgatg gtgatggcga acgtggtgtt agcatgccga ttccgcctga
     5761 agatctggtt cgtgcactgc gtgattatta tggtaaagtt gcagaatggg aagaaggctt
     5821 tgcagaagaa catcgtctga aacgtgcact ggaagtttat cgtgccctgc gcgaacgtct
     5881 gcaggatccg gaagcatttc cgctgcgtgt tggttttggt agcggtcgtc tggcactgcg
     5941 tctggccctg ctgctgccgg aagatcatcc tgaagcacaa gaaccgaaaa cccgtaaaac
     6001 cgcaggcgca cagaatccgg ttgatggtta tccgctgggt tggatggttg gtcgcctgga
     6061 accgctgagt gccctggaag ttctgttcca ggggccgcat catcaccacc atcaccatca
     6121 tcatcactaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     6181 tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc
     6241 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     6301 cgaccctgcc ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc ccctcggctt
     6361 gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
     6421 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     6481 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     6541 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     6601 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     6661 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     6721 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     6781 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     6841 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     6901 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     6961 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     7021 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     7081 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     7141 gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt
     7201 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7261 atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca
     7321 catacaaagt tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca
     7381 gcagggccgc gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa
     7441 acagacgctt ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta
     7501 cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg
     7561 cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg
     7621 gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg
     7681 ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg
     7741 taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat
     7801 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     7861 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
     7921 ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt
     7981 ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
     8041 tttgatcttt tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc
     8101 acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc
     8161 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     8221 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8281 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8341 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     8401 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     8461 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     8521 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     8581 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     8641 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     8701 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     8761 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     8821 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     8881 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     8941 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     9001 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     9061 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     9121 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     9181 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     9241 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     9301 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     9361 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     9421 gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata
//
