LOCUS       sequence-252588-       12391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB4 S774G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB4-S774G
FEATURES             Location/Qualifiers
     source          1..12391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7328..7352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7405..7446
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7467..8034
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7484..8036
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7628..7645)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7855..7874
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8048..8050,8051..8053,8054..8767)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8048..8050,8051..8053,8054..8764)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8093..8114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8354..8373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8701..8722
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8781..9369
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8834..8854)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9252..9263)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9444..9677
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9796..9814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9882..9986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9987..10055,10056..10847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10205..10224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11018..11606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11507..11526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11760..11777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12017..12038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12053..12058,12059..12076,12077..12083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12091..12107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12096..12118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12115..12131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12115..12131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12181..12200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg aagccggcga caggactttg ggtctgggtg
     3481 agccttctcg tggcggcggg gaccgtccag cccagcgatt ctcagtcagt gtgtgcagga
     3541 acggagaata aactgagctc tctctctgac ctggaacagc agtaccgagc cttgcgcaag
     3601 tactatgaaa actgtgaggt tgtcatgggc aacctggaga taaccagcat tgagcacaac
     3661 cgggacctct ccttcctgcg gtctgttcga gaagtcacag gctacgtgtt agtggctctt
     3721 aatcagtttc gttacctgcc tctggagaat ttacgcatta ttcgtgggac aaaactttat
     3781 gaggatcgat atgccttggc aatattttta aactacagaa aagatggaaa ctttggactt
     3841 caagaacttg gattaaagaa cttgacagaa atcctaaatg gtggagtcta tgtagaccag
     3901 aacaaattcc tttgttatgc agacaccatt cattggcaag atattgttcg gaacccatgg
     3961 ccttccaact tgactcttgt gtcaacaaat ggtagttcag gatgtggacg ttgccataag
     4021 tcctgtactg gccgttgctg gggacccaca gaaaatcatt gccagacttt gacaaggacg
     4081 gtgtgtgcag aacaatgtga cggcagatgc tacggacctt acgtcagtga ctgctgccat
     4141 cgagaatgtg ctggaggctg ctcaggacct aaggacacag actgctttgc ctgcatgaat
     4201 ttcaatgaca gtggagcatg tgttactcag tgtccccaaa cctttgtcta caatccaacc
     4261 acctttcaac tggagcacaa tttcaatgca aagtacacat atggagcatt ctgtgtcaag
     4321 aaatgtccac ataactttgt ggtagattcc agttcttgtg tgcgtgcctg ccctagttcc
     4381 aagatggaag tagaagaaaa tgggattaaa atgtgtaaac cttgcactga catttgccca
     4441 aaagcttgtg atggcattgg cacaggatca ttgatgtcag ctcagactgt ggattccagt
     4501 aacattgaca aattcataaa ctgtaccaag atcaatggga atttgatctt tctagtcact
     4561 ggtattcatg gggaccctta caatgcaatt gaagccatag acccagagaa actgaacgtc
     4621 tttcggacag tcagagagat aacaggtttc ctgaacatac agtcatggcc accaaacatg
     4681 actgacttca gtgttttttc taacctggtg accattggtg gaagagtact ctatagtggc
     4741 ctgtccttgc ttatcctcaa gcaacagggc atcacctctc tacagttcca gtccctgaag
     4801 gaaatcagcg caggaaacat ctatattact gacaacagca acctgtgtta ttatcatacc
     4861 attaactgga caacactctt cagcacaatc aaccagagaa tagtaatccg ggacaacaga
     4921 aaagctgaaa attgtactgc tgaaggaatg gtgtgcaacc atctgtgttc cagtgatggc
     4981 tgttggggac ctgggccaga ccaatgtctg tcgtgtcgcc gcttcagtag aggaaggatc
     5041 tgcatagagt cttgtaacct ctatgatggt gaatttcggg agtttgagaa tggctccatc
     5101 tgtgtggagt gtgaccccca gtgtgagaag atggaagatg gcctcctcac atgccatgga
     5161 ccgggtcctg acaactgtac aaagtgctct cattttaaag atggcccaaa ctgtgtggaa
     5221 aaatgtccag atggcttaca gggggcaaac agtttcattt tcaagtatgc tgatccagat
     5281 cgggagtgcc acccatgcca tccaaactgc acccaagggt gtaacggtcc cactagtcat
     5341 gactgcattt actacccatg gacgggccat tccactttac cacaacatgc tagaactccc
     5401 ctgattgcag ctggagtaat tggtgggctc ttcattctgg tcattgtggg tctgacattt
     5461 gctgtttatg ttagaaggaa gagcatcaaa aagaaaagag ccttgagaag attcttggaa
     5521 acagagttgg tggaaccatt aactcccagt ggcacagcac ccaatcaagc tcaacttcgt
     5581 attttgaaag aaactgagct gaagagggta aaagtccttg gctcaggtgc ttttggaacg
     5641 gtttataaag gtatttgggt acctgaagga gaaactgtga agattcctgt ggctattaag
     5701 attcttaatg agacaactgg tcccaaggca aatgtggagt tcatggatga agctctgatc
     5761 atggcaggta tggatcatcc acacctagtc cggttgctgg gtgtgtgtct gagcccaacc
     5821 atccagctgg ttactcaact tatgccccat ggctgcctgt tggagtatgt ccacgagcac
     5881 aaggataaca ttggatcaca actgctgctt aactggtgtg tccagatagc taagggaatg
     5941 atgtacctgg aagaaagacg actcgttcat cgggatttgg cagcccgtaa tgtcttagtg
     6001 aaatctccaa accatgtgaa aatcacagat tttgggctag ccagactctt ggaaggagat
     6061 gaaaaagagt acaatgctga tggaggaaag atgccaatta aatggatggc tctggagtgt
     6121 atacattaca ggaaattcac ccatcagagt gacgtttgga gctatggagt tactatatgg
     6181 gaactgatga cctttggagg aaaaccctat gatggaattc caacgcgaga aatccctgat
     6241 ttattagaga aaggagaacg tttgcctcag cctcccatct gcactattga cgtttacatg
     6301 gtcatggtca aatgttggat gattgatgct gacagtagac ctaaatttaa ggaactggct
     6361 gctgagtttt caaggatggc tcgagaccct caaagatacc tagttattca gggtgatgat
     6421 cgtatgaagc ttcccagtcc aaatgacagc aagttctttc agaatctctt ggatgaagag
     6481 gatttggaag atatgatgga tgctgaggag tacttggtcc ctcaggcttt caacatccca
     6541 cctcccatct atacttccag agcaagaatt gactcgaata ggaaccagtt tgtataccga
     6601 gatggaggtt ttgctgctga acaaggagtg tctgtgccct acagagcccc aactagcaca
     6661 attccagaag ctcctgtggc acagggtgct actgctgaga tttttgatga ctcctgctgt
     6721 aatggcaccc tacgcaagcc agtggcaccc catgtccaag aggacagtag cacccagagg
     6781 tacagtgctg accccaccgt gtttgcccca gaacggagcc cacgaggaga gctggatgag
     6841 gaaggttaca tgactcctat gcgagacaaa cccaaacaag aatacctgaa tccagtggag
     6901 gagaaccctt ttgtttctcg gagaaaaaat ggagaccttc aagcattgga taatcccgaa
     6961 tatcacaatg catccaatgg tccacccaag gccgaggatg agtatgtgaa tgagccactg
     7021 tacctcaaca cctttgccaa caccttggga aaagctgagt acctgaagaa caacatactg
     7081 tcaatgccag agaaggccaa gaaagcgttt gacaaccctg actactggaa ccacagcctg
     7141 ccacctcgga gcacccttca gcacccagac tacctgcagg agtacagcac aaaatatttt
     7201 tataaacaga atgggcggat ccggcctatt gtggcagaga atcctgaata cctctctgag
     7261 ttctccctga agccaggcac tgtgctgccg cctccacctt acagacaccg gaatactgtg
     7321 gtgtagaacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     7381 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     7441 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc
     7501 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     7561 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     7621 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     7681 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     7741 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     7801 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     7861 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     7921 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     7981 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     8041 cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     8101 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     8161 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     8221 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     8281 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     8341 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     8401 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     8461 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     8521 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     8581 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     8641 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     8701 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     8761 caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     8821 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8881 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8941 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     9001 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     9061 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9121 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     9181 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9241 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9301 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9361 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     9421 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     9481 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9661 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9721 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     9781 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     9841 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9961 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10021 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10081 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10141 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10201 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
    10261 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10321 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10381 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10441 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10501 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10561 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10621 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10681 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10741 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10801 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11821 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
    11881 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    11941 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    12001 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    12061 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    12121 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    12181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    12241 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    12301 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    12361 tttgttaaat atgtactaca aacttagtag t
//
