LOCUS       sequence-252625-        7900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Kv2.1in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Jorge BS, Campbell CM, Miller AR, Rutter ED, Gurnett CA, Vanoye CG, 
            George AL Jr, Kearney JA
  TITLE     Voltage-gated potassium channel KCNV2 (Kv8.2) contributes to 
            epilepsy susceptibility.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5443-8. doi: 
            10.1073/pnas.1017539108. Epub 2011 Mar 14.
  PUBMED    21402906
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1017539108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-29- 29"; volume: "108"; issue: "13"; pages: "5443-8"
COMMENT     Sequence Label: pIRES2-DsRed-MST-Kv2.1
FEATURES             Location/Qualifiers
     source          1..7900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    join(3303..3877,3878..3880,3881..3889)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3470..3487)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3697..3716
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3890..4567
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4020..4038)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4070..4090)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4288..4307
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4482..4505
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4688..4809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4725..4744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4779..4798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4816..5271)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4953..4974)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5165..5184
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5298..5402
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5404..5761
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5404..5595
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5427..5447)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5612..5747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5796..6590
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5796..6590
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5850..5869)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6460..6479
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6778..6797)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6822..6869
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7198..7786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7687..7706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagtaacg
      601 gccgccagtg tgctggaatt cgcccttggg ggcggccgga tcagcgatgc cggcgggcat
      661 gacgaagcat ggctcccgct ccaccagctc gctgccgccc gagcccatgg agatcgtgcg
      721 cagcaaggcg tgctctcggc gggtccgcct caacgtcggg gggctggcgc acgaggtact
      781 ctggcgtacc ctggaccgcc tgccccgcac gcggctgggc aagctccgcg actgcaacac
      841 gcacgactcg ctgctcgagg tgtgcgatga ctacagcctc gacgacaacg agtacttctt
      901 tgaccgccac ccgggcgcct tcacctccat cctcaacttc taccgcactg ggcgactgca
      961 catgatggag gagatgtgcg cgctcagctt cagccaagag ctcgactact ggggcatcga
     1021 cgagatctac ctggagtcct gctgccaggc ccgctaccac cagaagaaag agcagatgaa
     1081 cgaggagctc aagcgtgagg ccgagaccct acgggagcgg gaaggcgagg agttcgataa
     1141 cacgtgctgc gcagagaaga ggaaaaaact ctgggaccta ctggagaagc ccaattcctc
     1201 tgtggctgcc aagatccttg ccataatttc catcatgttc atcgtcctct ccaccattgc
     1261 cctgtccctc aacacgctgc ctgagctaca gagcctcgat gagttcggcc agtccacaga
     1321 caacccccag ctggcccacg tggaggccgt gtgcatcgca tggttcacca tggagtacct
     1381 gctgaggttc ctctcctcgc ccaagaagtg gaagttcttc aagggcccac tcaatgccat
     1441 tgacttgttg gccattctgc catactatgt caccattttc ctcaccgaat ccaacaagag
     1501 cgtgctgcaa ttccagaatg tccgccgcgt ggtccagatc ttccgcatca tgcgaattct
     1561 ccgcatcctt aagcttgcac gccactccac tggcctccag tctctgggct tcactttgcg
     1621 gaggagctac aatgagttgg gcttgctcat cctcttcctt gccatgggca ttatgatctt
     1681 ctccagcctt gtcttctttg ctgagaagga tgaggacgac accaagttca aaagcatccc
     1741 agcctctttc tggtgggcca ccatcaccat gactactgtt gggtatggag acatctaccc
     1801 caagactctc ctggggaaaa ttgttggggg actctgctgc attgcaggag tcctggtgat
     1861 tgctcttccc atccccatca tcgtcaataa cttctctgag ttctataagg agcagaagag
     1921 acaggagaaa gcaatcaaac ggcgagaggc tctggagaga gccaagagga atggcagcat
     1981 cgtatccatg aacatgaagg atgcttttgc ccggagcatt gagatgatgg acattgtggt
     2041 tgagaaaaat ggggagaata tgggtaagaa agacaaagta caagataacc acttgtctcc
     2101 taacaaatgg aaatggacaa agaggacact gtctgaaacc agctcaagta agtcctttga
     2161 aaccaaggaa cagggatccc ctgaaaaagc cagatcgtct tctagtcctc agcacctgaa
     2221 cgttcagcag ttggaagaca tgtacaataa gatggccaag acccaatccc aacccatcct
     2281 caataccaag gagtcagcag cacagagcaa accaaaggaa gaacttgaaa tggagagtat
     2341 ccccagcccc gtagcccctc tgcccactcg cacagaaggg gtcattgaca tgcgaagtat
     2401 gtcaagcatt gatagtttca ttagctgtgc cacagacttc cctgaggcca ccagattctc
     2461 ccacagccct ttgacatcac tccccagcaa gactgggggc agcacagccc cagaagtggg
     2521 ctggcgggga gctctgggtg ccagtggtgg taggtttgtg gaggccaacc ccagccctga
     2581 tgccagccag cactctagtt tcttcatcga gagccccaag agttccatga aaactaacaa
     2641 ccctttgaag ctccgagcac ttaaagtcaa cttcatggag ggtgacccca gtccactcct
     2701 ccccgttcta gggatgtacc atgaccctct caggaaccgg gggagtgccg cggctgctgt
     2761 cgctggactg gagtgtgcca cgcttttgga caaggctgtg ctgagcccag agtcctccat
     2821 ctacaccaca gcaagtgcta agacaccccc ccggtctcct gagaaacaca cagcaatagc
     2881 gttcaacttt gaggcgggtg tccaccagta cattgacgca gacacagatg atgagggaca
     2941 gctgctctac agtgtggact ccagcccccc caaaagcctc cctgggagca ccagtccgaa
     3001 gttcagcacg gggacaagat cggagaaaaa ccactttgaa agctcccctt tacccacctc
     3061 ccctaagttc ttaaggcaga actgtattta ctccacagaa gcattgactg gaaaaggccc
     3121 cagtggtcag gaaaagtgca aacttgagaa ccacatctcc cctgacgtcc gtgtgttgcc
     3181 agggggagga gcccatggaa gcacacgaga tcagagcatc tgaactgccc tgcctaaggg
     3241 cgaattctgc agatatccat cacactggcg gccgctcgac ggtaccgcgg gcccgggatc
     3301 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3361 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3421 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3481 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3541 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3601 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3661 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3721 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3781 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3841 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggcctcctc
     3901 cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
     3961 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4021 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     4081 gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa
     4141 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4201 ggtgaccgtg acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt
     4261 catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacta tgggctggga
     4321 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc
     4381 cctgaagctg aaggacggcg gccactacct ggtggagttc aagtctatct acatggccaa
     4441 gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca
     4501 caacgaggac tacaccatcg tggagcagta cgagcgcgcc gagggccgcc accacctgtt
     4561 cctgtagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     4621 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4681 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4741 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4801 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4861 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4921 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4981 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5041 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     5101 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     5161 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     5221 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     5281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5401 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5461 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5581 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5641 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5701 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5761 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5821 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5881 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5941 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     6001 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6061 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6121 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6181 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6241 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6301 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     6361 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6421 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6481 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6541 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6601 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6661 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6721 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     6781 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6841 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     6901 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     6961 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     7021 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     7081 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7141 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7201 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7261 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7321 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7381 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7441 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7501 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7561 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7621 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7681 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7741 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7801 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7861 atcccctgat tctgtggata accgtattac cgccatgcat
//
