LOCUS       sequence-252647-        4505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme 
            converting benzaldehyde to benzoate) under control of the 
            constitutive promoter J23101, and RBS B0032.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pBEAST_J23101-vdh
FEATURES             Location/Qualifiers
     source          1..4505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      148..175
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        301..335
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      1979..2050
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2066..2093
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2320..2337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2491..3079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2571..2590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3250..4041,4042..4110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3873..3892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4111..4215)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4283..4301
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct gttccgctgg
       61 ctcggatacc cttactctgt tgaaaacgaa tagataggtt aaggaacggt tatttctgcg
      121 tagatctatc ttacacagca tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      181 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttaggca
      241 actgaaacga ttcggatcct gtattactat tcttaggata ctagaggatg accccatctg
      301 tttacagcta gctcagtcct aggtattatg ctagctagta gagtcacaca ggaaagtagt
      361 agatgcacaa cgttcagctg aaacaggaca acaccgttga cacctcttct ttcgaatctg
      421 ctccgaacgt tcacaccgtt cagctgctga tccacggtca gtctgttgac gcttctaacc
      481 agatgacctt caaacgtatc tctccgatcg acggtcaggt tgcttctatc gctgctgctg
      541 ctaccctggc tgacgttgac ctggctatcg aatctgctgc taaagctttc ccgatctggt
      601 ctaaactgtc tccgaccgaa cgtcgtctgc gtctgctgaa agctgctgac ctgatggacg
      661 ctcgtaccga ccagttcatc cagatcggta tgcgtgaaac cggttctacc gctacctggt
      721 acggcttcaa cgttcacctc gctgctaaca tgctgcgtga agctgctgct atgaccaccc
      781 agatggacgg ttctctgatc ccgtctgacg ttccgggtaa catggctatg ggtatccgtg
      841 ttccgtgcgg tgttgttgtt ggtatcgctc cgtggaacgc tccggttatc ctgccgaccc
      901 gtgcactggc tatgccgctg gcttgcggta acaccgttgt tctgaaagct tctgaagctt
      961 gcccggctac ccagcgtctg atcggtcagg ttctgcacga agctggtctg ggtgacggtg
     1021 ttgttaacgt tatcacccac gctgctgaag acgcttctca gatcgttgaa cgtctgatct
     1081 ctcacccggc tgttaaacgt atcaacttca ccggttctac caacgttggt aaaatcatcg
     1141 ctgaaaccgc tgctaaatac ctgaaaccgg ttctgctgga actgggtggt aaagctccgg
     1201 ttgttgttct gaacgaagct gacgttgacg aagctgttaa cgctgttgtt ttcggtgctt
     1261 tcttcaacca gggtcagatc tgcatgtcta ccgaacgtgt tctggttcag gaccgtatcg
     1321 ctgaccagtt catcgaaaaa ctgatcgaaa aaacccgtac catccacgct ggtaacccga
     1381 ccttcaaagg tcacgttctg ggtgttctgg aatctcagcg tgctgctaac cgtatccagc
     1441 acctgctgga agacgctcag tctcagggtg ctgacctgcc gctgggtatc cacatccaga
     1501 acaccaccat gcagccgacc ctggttctga acatccagcc ggaaatgctg ctgtaccgtg
     1561 aagaatcttt cggtccggtt tgcaccgttc agcgtttcaa ctctgttgaa gaaggtatcg
     1621 ccctggctaa cgactctgaa ttcggtctgt ctgctgctgt tttctctcag gacatcgctc
     1681 aggccctgga cgttgctaaa cagatcgact ccggtatctg ccacatcaac ggtgctaccg
     1741 ttcacgacga agctcagatg ccgttcggtg gtaccaaagc ttctggttac ggtcgtttcg
     1801 gttctaaagc ttctatcgct gaattcaccg aactgcgttg gatcaccatc cagacccagt
     1861 ctcgtcacta cccgatctaa gcttacttta tctgagaata gtcaatcttc ggaaatccca
     1921 ggtggcatgc taaaagtctc gtaaagcgtt ctatcaataa cccgttggtg ccaggcatca
     1981 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2041 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatac
     2101 cgtctcagaa tcggccgtga acaataaaat agtttcggta ttattgacca cttccgagta
     2161 gaatcgtgct tcagtaagag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     2221 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     2281 caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg
     2341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3841 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     3901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4261 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     4321 ctggcgaatc ctctgaccag ccagaaaacg acctttctgt ggtgaaaccg gatgctgcaa
     4381 ttcagagcgc cagcaagtgg gggacagcag aagacctgac cgccgcagag tggatgtttg
     4441 acatggtgaa gactatcgca ccatcagcca gaaaaccgaa ttttgctggg tgggctaacg
     4501 atatc
//
