LOCUS       sequence-252648-        3851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme 
            converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) 
            under control of the constitutive promoter J23101, and RBS B0032.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pBEAST_J23101-bphD
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      148..175
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        301..335
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      1325..1396
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1412..1439
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1666..1683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1837..2425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1917..1936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2596..3387,3388..3456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3219..3238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3457..3561)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3629..3647
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct gttccgctgg
       61 ctcggatacc cttactctgt tgaaaacgaa tagataggtt aaggaacggt tatttctgcg
      121 tagatctatc ttacacagca tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      181 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttaggca
      241 actgaaacga ttcggatcct gtattactat tcttaggata ctagaggatg accccatctg
      301 tttacagcta gctcagtcct aggtattatg ctagctagta gagtcacaca ggaaagtagt
      361 agatgacagc attgactgaa agctctacta gcaaattcct taacatcaaa gagaaaggct
      421 tgtccgactt taagattcat tataatgaag cgggcaacgg tgaaactgtc atcatgctgc
      481 atggcggtgg accgggagcc ggaggatggt cgaactatta tcgtaatatc ggaccgttcg
      541 ttgaagccgg ttaccgtgtc attttgaagg attcacccgg ctttaacaaa tccgatgctg
      601 tcgtcatgga tgaacaacgt gggcttgtaa atgctcgtgc ggtcaaggga ttgatggatg
      661 ctcttggcat tgatcgtgcg catctggtgg gaaattcaat gggaggtgca accgcgctta
      721 acttcgccat cgagtatcca gaccgtattg gaaaacttat ccttatgggt ccgggaggtt
      781 tgggaccctc catgtttgcc ccaatgccct tagagggaat taaattatta tttaagttat
      841 atgcagagcc gtcgtatgaa aatctgaaac agatgatcca agtgttcctt tatgatcaat
      901 ctctgattac tgaggaactt ttacaaggac gctgggaagc cattcagcgt caaccagaac
      961 atcttaaaaa cttcctgatt tctgcgcaga aggcgcccct gagtacgtgg gatgttaccg
     1021 cccgtttggg agagattaag gcgaagacct tcattacatg gggtcgtgac gaccgcttcg
     1081 tgccgttaga ccatggtctg aaacttttgt ggaatattga tgacgcacgc ttgcacgttt
     1141 tttccaagtg cggacattgg gcacaatggg agcatgctga cgagtttaac cgcttagcca
     1201 ttgactttct gcgccaggct taatgagctt actttatctg agaatagtca atcttcggaa
     1261 atcccaggtg gcatgctaaa agtctcgtaa agcgttctat caataacccg ttggtgccag
     1321 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1381 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1441 ttataccgtc tcagaatcgg ccgtgaacaa taaaatagtt tcggtattat tgaccacttc
     1501 cgagtagaat cgtgcttcag taagagtcga ccgatgccct tgagagcctt caacccagtc
     1561 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     1621 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     1681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3181 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     3241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     3601 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     3661 agaattctgg cgaatcctct gaccagccag aaaacgacct ttctgtggtg aaaccggatg
     3721 ctgcaattca gagcgccagc aagtggggga cagcagaaga cctgaccgcc gcagagtgga
     3781 tgtttgacat ggtgaagact atcgcaccat cagccagaaa accgaatttt gctgggtggg
     3841 ctaacgatat c
//
