LOCUS       sequence-252649-        3872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme 
            converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) 
            under control of the constitutive promoter J23101, and RBS B0032.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pBEAST_J23101-bphC
FEATURES             Location/Qualifiers
     source          1..3872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      148..175
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        301..335
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      1346..1417
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1433..1460
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1687..1704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1858..2446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1938..1957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2617..3408,3409..3477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3240..3259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3478..3582)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3650..3668
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct gttccgctgg
       61 ctcggatacc cttactctgt tgaaaacgaa tagataggtt aaggaacggt tatttctgcg
      121 tagatctatc ttacacagca tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      181 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttaggca
      241 actgaaacga ttcggatcct gtattactat tcttaggata ctagaggatg accccatctg
      301 tttacagcta gctcagtcct aggtattatg ctagctagta gagtcacaca ggaaagtagt
      361 agatgagcat tgaacgctta ggttacctgg gtttcgcagt gaaagatgtg ccagcctggg
      421 accactttct gacgaaatcc gtgggcttaa tggcggccgg tagcgccgga gatgcagccc
      481 tttaccgtgc ggaccaacgt gcttggcgca tcgcagtaca acctggtgag cttgacgatt
      541 tagcctatgc aggcttagag gtggacgacg cagctgcgct tgaacgtatg gcggacaaat
      601 tacgtcaagc tggtgttgcg ttcacccgtg gggacgaggc cctgatgcaa cagcgcaaag
      661 tgatggggct tctttgcttg caggatccat ttggattacc tttggaaatc tattatggac
      721 ctgctgaaat tttccacgaa ccattcttgc cgtctgctcc tgtttccggg ttcgtgaccg
      781 gggaccaggg tattggccat tttgtccgtt gtgttcccga tacagcgaag gctatggctt
      841 tttacaccga ggtccttggg ttcgtgcttt cagacattat tgacattcaa atggggcccg
      901 agacttccgt tcccgctcac ttcttacatt gcaacggacg ccatcacact atcgctttgg
      961 ccgcctttcc cattccgaaa cgtatccacc acttcatgtt acaggcaaac acaatcgacg
     1021 acgtgggtta cgcatttgat cgtctggatg cagcagggcg cattacctcg ctgctggggc
     1081 gtcacaccaa tgatcagacc ctgagctttt acgctgatac cccaagcccc atgattgagg
     1141 tcgaattcgg ttggggcccg cgtacagtgg attcctcttg gaccgtagcg cgtcactcgc
     1201 gcaccgctat gtgggggcat aagtctgttc gcggacaacg ctaatgagct tactttatct
     1261 gagaatagtc aatcttcgga aatcccaggt ggcatgctaa aagtctcgta aagcgttcta
     1321 tcaataaccc gttggtgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1381 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1441 ttcgggtggg cctttctgcg tttataccgt ctcagaatcg gccgtgaaca ataaaatagt
     1501 ttcggtatta ttgaccactt ccgagtagaa tcgtgcttca gtaagagtcg accgatgccc
     1561 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     1621 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     1681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     3241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3601 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3661 cacgaggccc tttcgtcttc aagaattctg gcgaatcctc tgaccagcca gaaaacgacc
     3721 tttctgtggt gaaaccggat gctgcaattc agagcgccag caagtggggg acagcagaag
     3781 acctgaccgc cgcagagtgg atgtttgaca tggtgaagac tatcgcacca tcagccagaa
     3841 aaccgaattt tgctgggtgg gctaacgata tc
//
