LOCUS       sequence-252650-        4556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The cell-free adapted backbone, pBEAST, expressing the amidase 
            enzyme gene (benzamid to benzoate) under control of the constitutive
            promoter J23101, and RBS B0032.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pBEAST_J23101-amidase
FEATURES             Location/Qualifiers
     source          1..4556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      148..175
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        301..335
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      2030..2101
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2117..2144
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2371..2388)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2542..3130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2622..2641)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3301..4092,4093..4161))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3924..3943
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4162..4266)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4334..4352
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct gttccgctgg
       61 ctcggatacc cttactctgt tgaaaacgaa tagataggtt aaggaacggt tatttctgcg
      121 tagatctatc ttacacagca tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      181 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttaggca
      241 actgaaacga ttcggatcct gtattactat tcttaggata ctagaggatg accccatctg
      301 tttacagcta gctcagtcct aggtattatg ctagctagta gagtcacaca ggaaagtagt
      361 agatggcgac aatccgtccc gatgacaacg caattgacac ggcggcccgc cattatggca
      421 tcacccttga ccaaagcgcg cgtcttgagt ggcccgcact tattgacgga gccttaggga
      481 gctacgacgt tgttgaccag ctgtacgctg atgaagccac gccgccaaca acgtcgcgtg
      541 aacatactgt ccctactgct agcgaaaatc ccctttccgc ctggtacgtt acgacctcta
      601 tcccccccac aagtgacgga gtgttgactg gacgccgcgt cgccatcaaa gataacgtca
      661 cagtagctgg cgtgccaatg atgaacggct cgcgtaccgt tgagggattt actccgtcac
      721 gcgacgccac tgtagtcact cgcctgctgg ctgctggtgc aacagtagct ggaaaggctg
      781 tctgtgagga cttatgcttt tctggctcta gttttacccc agcctcggga cctgttcgca
      841 atccctggga tccgcagcgc gaggcaggag gaagttccgg cggaagtgca gcattagtag
      901 caaatggcga tgtcgacttc gcaattggag gtgaccaggg tggctccatc cgtatcccgg
      961 ctgccttttg cggcgtagtc ggccacaagc ctacatttgg acttgtacca tatacgggag
     1021 ccttcccaat cgaacgcacg attgaccacc ttggaccgat tacacgcact gtccatgacg
     1081 ctgcacttat gctgtcagtt atcgcaggcc gcgatggaaa cgaccctcgt caagcggata
     1141 gtgtggaagc gggcgactac cttagtactt tagatagcga cgtcgacggg ttacgtatcg
     1201 gaatcgtacg tgagggtttt ggccacgcag tcagccaacc ggaggtagac gacgcggttc
     1261 gtgcagcggc tcacagctta gcagaaatcg gatgcacagt ggaagaagtg aacattccat
     1321 ggcacctgca tgcgtttcat atctggaatg tgattgccac cgatggcggt gcttaccaaa
     1381 tgttagacgg gaacggttat ggaatgaatg cagaaggttt atacgaccct gaacttatgg
     1441 ctcacttcgc atctcgtcgt cttcaacatg cagatgcctt gtctgaaacc gttaagcttg
     1501 tagctctgac cggccaccac gggattacga cattaggggg cgcttcgtac gggaaagccc
     1561 gcaacttggt tccgttagcg cgtgcagctt acgacaccgc gcttcgtcag ttcgacgtgc
     1621 ttgtaatgcc aactttacct tatgtcgcct cagaattacc agccaatgat gtcgaccgtg
     1681 caacttttat tactaaggcg cttggtatga tcgctaacac agcacctttc gatgtaacag
     1741 ggcacccgag cttatcagtt ccagctggcc ttgtaaatgg gttacctgtc ggtatgatga
     1801 ttactggaaa gacttttgat gatgcgacag tgcttcgtgt agggcgtgcc tttgagaaat
     1861 tacgtggggc ctttccgacc cctgcagatc acatttcgga tagtgccccg caattaagcc
     1921 ctgcgtaatg agcttacttt atctgagaat agtcaatctt cggaaatccc aggtggcatg
     1981 ctaaaagtct cgtaaagcgt tctatcaata acccgttggt gccaggcatc aaataaaacg
     2041 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2101 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata ccgtctcaga
     2161 atcggccgtg aacaataaaa tagtttcggt attattgacc acttccgagt agaatcgtgc
     2221 ttcagtaaga gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     2281 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     2341 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2401 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2461 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2941 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3421 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3601 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3901 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     3961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4141 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4261 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4321 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tctggcgaat
     4381 cctctgacca gccagaaaac gacctttctg tggtgaaacc ggatgctgca attcagagcg
     4441 ccagcaagtg ggggacagca gaagacctga ccgccgcaga gtggatgttt gacatggtga
     4501 agactatcgc accatcagcc agaaaaccga attttgctgg gtgggctaac gatatc
//
