LOCUS       sequence-252651-        4142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme 
            converting hippurate to benzoate) under control of the constitutive 
            promoter J23101, and RBS B0032.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pBEAST_J23101-HipO
FEATURES             Location/Qualifiers
     source          1..4142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      148..175
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        301..335
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      1616..1687
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1703..1730
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1957..1974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2128..2716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2208..2227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2887..3678,3679..3747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3510..3529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3748..3852)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3920..3938
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct gttccgctgg
       61 ctcggatacc cttactctgt tgaaaacgaa tagataggtt aaggaacggt tatttctgcg
      121 tagatctatc ttacacagca tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      181 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttaggca
      241 actgaaacga ttcggatcct gtattactat tcttaggata ctagaggatg accccatctg
      301 tttacagcta gctcagtcct aggtattatg ctagctagta gagtcacaca ggaaagtagt
      361 agatgatgaa cctgatcccg gaaatcctgg acctgcaggg tgaattcgaa aaaatccgtc
      421 accagatcca cgaaaacccg gaactgggtt tcgacgaact gtgcaccgct aaactggttg
      481 ctcagaaact gaaagaattc ggttacgaag tttacgaaga aatcggtaaa accggtgttg
      541 ttggtgttct gaaaaaaggt aactctgaca aaaaaatcgg tctgcgtgct gacatggacg
      601 ctctgccgct gcaggaatgc accaacctgc cgtacaaatc taaaaaagaa aacgttatgc
      661 acgcttgcgg tcacgacggt cacaccacct ctctgctgct ggctgctaaa tacctggctt
      721 ctcagaactt caacggtgct ctgaacctgt acttccagcc ggctgaagaa ggtctgggtg
      781 gtgctaaagc tatgatcgaa gacggtctgt tcgaaaaatt cgactctgac tacgttttcg
      841 gttggcacaa catgccgttc ggttctgaca aaaaattcta cctgaaaaaa ggtgctatga
      901 tggcttcttc tgactcttac tctatcgaag ttatcggtcg tggtggtcac ggttctgctc
      961 cggaaaaagc taaagacccg atctacgctg cttctctgct gatcgttgct ctgcagtcta
     1021 tcgtttctcg taacgttgac ccgcagaact ctgctgttgt ttctatcggt gctttcaacg
     1081 ctggtcacgc tttcaacatc atcccggaca tcgctaccat caaaatgtct gttcgtgctc
     1141 tggacaacga aacccgtaaa ctgaccgaag aaaaaatcta caaaatctgc aaaggtatcg
     1201 ctcaggctaa cgacatcgaa atcaaaatca acaaaaacgt tgttgctccg gttaccatga
     1261 acaacgacga agctgttgac ttcgcttctg aagttgctaa agaactgttc ggtgaaaaaa
     1321 actgcgaatt caaccaccgt ccgctgatgg cttctgaaga cttcggtttc ttctgcgaaa
     1381 tgaaaaaatg cgcttacgct ttcctggaaa acgaaaacga catctacctg cacaactctt
     1441 cttacgtttt caacgacaaa ctgctggctc gtgctgcttc ttactacgct aaactggctc
     1501 tgaaatacct gaaataagct tactttatct gagaatagtc aatcttcgga aatcccaggt
     1561 ggcatgctaa aagtctcgta aagcgttcta tcaataaccc gttggtgcca ggcatcaaat
     1621 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1681 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataccgt
     1741 ctcagaatcg gccgtgaaca ataaaatagt ttcggtatta ttgaccactt ccgagtagaa
     1801 tcgtgcttca gtaagagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1861 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1921 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2761 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2821 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2881 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2941 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3001 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3061 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3121 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3181 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3241 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3301 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3361 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3421 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3481 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     3541 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3601 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3661 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3721 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3781 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3841 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3901 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattctg
     3961 gcgaatcctc tgaccagcca gaaaacgacc tttctgtggt gaaaccggat gctgcaattc
     4021 agagcgccag caagtggggg acagcagaag acctgaccgc cgcagagtgg atgtttgaca
     4081 tggtgaagac tatcgcacca tcagccagaa aaccgaattt tgctgggtgg gctaacgata
     4141 tc
//
