LOCUS       sequence-252714-       10480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Ramalho JJ, Sepers JJ, Nicolle O, Schmidt R, Cravo J, Michaux G, 
            Boxem M
  TITLE     C-terminal phosphorylation modulates ERM-1 localization and dynamics
            to control cortical actin organization and support lumen formation 
            during Caenorhabditiselegans development.
  JOURNAL   Development. 2020 Jul 22;147(14):dev188011. doi: 10.1242/dev.188011.
  PUBMED    32586975
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-07-22"; volume: "147(14):dev188011. 
            doi"; pages: " 10.1242/dev.188011"
COMMENT     Sequence Label: pJJR82
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             join(1038..1340,1394..1468,1520..1639,1691..1906)
                     /codon_start=1
                     /product="monomeric enhanced GFP, optimized with synthetic 
                     introns for expression in C. elegans"
                     /label=mEGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGMDELYK"
     CDS             1907..1927
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1945..1978)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1988..2017
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3834..4157)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6075..6099
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6102..7127
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6102..7127
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7128..7152)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7505..7538)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7557..7580
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7584..7607
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7611..7634
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7682..7987)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8259..8275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8259..8275)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8272..8294)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8283..8299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8307..8313,8314..8331,8332..8337))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8352..8373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8490..8507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8661..9249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8741..8760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9420..10211,10212..10280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10043..10062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10281..10385)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10453..10471
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg tccaagggag aggagctctt caccggagtc gtcccaatcc
     1081 tcgtcgagct cgacggagac gtcaacggac acaagttctc cgtctcagga gagggagagg
     1141 gagacgccac ctacggaaag ctcaccctca agttcatctg caccaccgga aagctcccag
     1201 tcccatggcc aaccctcgtc accaccttca cttacggagt ccaatgcttc tcccgttacc
     1261 cagaccacat gaagcgtcac gacttcttca agtccgccat gccagaggga tacgtccaag
     1321 agcgtaccat cttcttcaag gtaagtttaa acattaatta atactaacta accctgatta
     1381 tttaaatttt caggacgacg gaaactacaa gacccgtgcc gaggtcaagt tcgagggaga
     1441 caccctcgtc aaccgtatcg agctcaaggt aagtttaaac agttcggtac taactaacca
     1501 tacatattta aattttcagg gaatcgactt caaggaggac ggaaacatcc tcggacacaa
     1561 gctcgaatac aactacaact cccacaacgt ctacatcatg gccgacaagc aaaagaacgg
     1621 aatcaaggtc aacttcaagg taagtttaaa catgatttta ctaactaact aatctgattt
     1681 aaattttcag atccgtcaca acatcgagga cggatctgtc caactcgccg accactacca
     1741 acaaaacacc ccaatcggag acggaccagt cctcctccca gacaaccact acctctccac
     1801 ccaatccgcc ctctccaagg acccaaacga gaagcgtgac cacatggtcc tcaaggagtt
     1861 cgtcaccgct gccggaatca cccacggaat ggacgagctc tacaaggaga atctgtactt
     1921 tcaatccgga aaggtaagtt taaaataact tcgtatagca tacattatac gaagttattt
     1981 tcagggagag caaaagctta tctccgagga ggacctctaa ggatgatcga cgccaacgtc
     2041 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
     2101 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
     2161 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     2221 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     2281 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     2341 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaacta
     2401 gctgccctcg attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt
     2461 cttctctggt tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca
     2521 cattcctcac ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc
     2581 tgcgtctcgc acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact
     2641 tgcggaaaga tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct
     2701 tctcgaagac taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga
     2761 taatttcaga gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca
     2821 ctcttatggt atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag
     2881 agaagcttga taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa
     2941 ataattttta attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg
     3001 aagatctagc aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca
     3061 tgctgatgga tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa
     3121 ccagccaaag actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga
     3181 taacaagtgc ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga
     3241 cggagttcca ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg
     3301 cgagtctgtc ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg
     3361 aagaccagga ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga
     3421 tggacaacca ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga
     3481 gccaggacca ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc
     3541 aaagggacca agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg
     3601 accaccagga gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca
     3661 aggaccagac ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga
     3721 gtactgccag tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata
     3781 cagaaatatc taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt
     3841 ttcttggcgg cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa
     3901 atatttgagc gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt
     3961 gaagagagat tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc
     4021 agatgcaaac ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat
     4081 aaattagcga gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc
     4141 ttgaaaggat tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag
     4201 tttcagaatc caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg
     4261 cttgcataat ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac
     4321 gggcggaatg tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga
     4381 tgagacggtg ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa
     4441 tatgttgttt aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg
     4501 cagcaactcc gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc
     4561 cggaaaccga aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga
     4621 ctccggcagt cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt
     4681 ctgtgcgact aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt
     4741 tgtaagcaat tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca
     4801 taagagaacg gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat
     4861 acattaatat ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat
     4921 caacattttc cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc
     4981 gtggaagtcc agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct
     5041 tcaccgccaa tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg
     5101 ctgggaacca cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga
     5161 gaagcatctt ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt
     5221 tcttgcgaac ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag
     5281 tgagaagatt tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct
     5341 tcctctgagc ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa
     5401 ggtcctagat gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa
     5461 atgtatctgg gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc
     5521 tccttttgca acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg
     5581 ttgaaaataa ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat
     5641 aatcatgtca ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt
     5701 ttgctttcgt cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc
     5761 aaattcgttg actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg
     5821 catcgggaaa tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt
     5881 ttgctatgtt ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct
     5941 ttgcatgtaa ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg
     6001 tttattttgt tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata
     6061 tgtcaggcgg tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca
     6121 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6181 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6241 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6301 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6361 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6421 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6481 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6541 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6601 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6661 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6721 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6781 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     6841 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     6901 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     6961 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     7021 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7081 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt
     7141 gtacaaagtg ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     7201 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     7261 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     7321 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     7381 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     7441 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     7501 tttaataact tcgtatagca tacattatac gaagttattt tcagggagcc ggatctgatt
     7561 ataaagacga tgacgataag cgtgactaca aggacgacga cgacaagcgt gattacaagg
     7621 atgacgatga caagagacta gttcgacctg cagactggct gtgtataagg gagcctgaca
     7681 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     7741 gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca
     7801 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacggggg actttatctg
     7861 acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat
     7921 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
     7981 actgcatttc accagcccct gttctcgtcg gcaaaagagc cgttcatttc aataaaccgg
     8041 gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg
     8101 gcttcattct gcatggttgt gcttaccgaa ccggagatat tgacatcata tatgccttga
     8161 gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct
     8221 ttttgacata cttcgggttc ctaggaaatc gataaatggt catagctgtt tcctgtgtga
     8281 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8341 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8401 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8461 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8521 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8581 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8641 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8701 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8821 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8881 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9001 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9061 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9121 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9181 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9301 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9361 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9421 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9481 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9541 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9601 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9661 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9721 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9781 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9841 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9901 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9961 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10021 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10081 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10141 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10201 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10261 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10321 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10381 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10441 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
