LOCUS       sequence-252745-       11210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length mouse archvillin (Supervillin, 
            isoform 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Oh SW, Pope RK, Smith KP, Crowley JL, Nebl T, Lawrence JB, Luna EJ
  TITLE     Archvillin, a muscle-specific isoform of supervillin, is an early 
            expressed component of the costameric membrane skeleton.
  JOURNAL   J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-75.
  PUBMED    12711699
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2003 Jun 1. 116(Pt 11):2261-75."
COMMENT     Sequence Label: pEGFP-mAV
FEATURES             Location/Qualifiers
     source          1..11210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..441
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        442..645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     596..616
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(690..692,693..695,696..1406)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(690..692,693..695,696..1406)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(735..756)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(996..1015)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1343..1364
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8075..8196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8112..8131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8166..8185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8203..8658)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8340..8361)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8685..8789
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8791..9148
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8791..8982
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8814..8834)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8999..9134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9183..9977
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9183..9977
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9237..9256)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9847..9866
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10165..10184)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10209..10256
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10585..11173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11074..11093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
       61 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
      121 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      181 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      241 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      301 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      361 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      421 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      481 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      541 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      601 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      661 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
      721 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      781 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      841 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      901 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      961 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1021 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1081 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1141 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1201 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1261 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1321 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1381 ctctcggcat ggacgagctg tacaagtact cagatctcga gatgaaaaga aaagaaagaa
     1441 ttgctaggcg cttagaagga atagaaaatg actcccagcc catcctgttg cagagctgca
     1501 cagggctagt gactcatcgg ctgctagaag aagacacacc cagatacatg cgtgccacag
     1561 accctgcaag ccctcatatt gggcgatcca aagaagagga agacactcct gggtcctccc
     1621 tagaaaagca aactccttct aaatactgca tagaaacctc aggcatccat agttcgggct
     1681 ccatggacac ccacagtctg gagtccaaag cggaaaggat cgcaagatac aaagcagaga
     1741 ggaggcggca gctggccgaa aagtacgggc tgaccttgga cccagaagct gactctgaat
     1801 acttgtctcg atatgccaag tctcggaagg acccagatgt gactgagaga cgaggaaaaa
     1861 gtgacaagca agaagaacag agcaaggatg ccaattcccg gcactccagg acagagtcag
     1921 ggcccaggac cagcttggtg gcgtcccagg actgtacccc gctgggaagc aatatgtctg
     1981 accaggagca gctgctcaat gtggagaacc agagaagagt ccaagatccc ccactaggag
     2041 aagatggctc ctctgccttc ttttctgagc gaagcatttc cttccccgaa gtgcccaggt
     2101 ctccaaagca gatacccagc tcaccactgc aacagccagc ctccccaaac caccccggtg
     2161 actcaccact gcccacagag gcccgagcca gtacagggaa gcccacacat gagtggtttc
     2221 tgcagagaga ttctgaaggc gatacacctt cacttatcaa ctggccttcc agagttaaag
     2281 ttagagaaaa attggtgaaa gaggagagtg ctcgcagcag ccctgaactc acctcagagt
     2341 ccttaactca gaggagacag cagccggcac cagcacattt cctgcctatc cagtcagaaa
     2401 gttccacctt tgatagggtc accagcaaag cagtgagctc attgcagcca agccagagtg
     2461 gtgttctgcc aactgaccct gttcatgcca tcaagctggt gaccatggat accccagaaa
     2521 gcacatctga attcagctgg gtggggtcag ccaccccaaa agttataaaa tctaccacct
     2581 tgaagattct agaaggtggt tcaagggatg ctccagtact ccatatttgt gagtcaaaag
     2641 cagaagattg gctctcccca gaacctctgg agaggagccc caagtcactt ttgacctcag
     2701 aagatgacag gctcgtgaga ggccacaaag atccctctgg taataaagac ttggacaagg
     2761 ccatcatttg ctccatcgat gtagaatctg agagggagcg gcaagttcaa cacctgccca
     2821 ctcagagaac tggccggagt gaaatgctcc tgtatgttca gagtggtcct gtgtcccaag
     2881 atgccacatt gaccagtcac acaaaggagg catctccaaa gaagcgcaag gttctggccc
     2941 gctctctgtc agattacaca ggtccccctc agctccaggt ccccagacac aaggatgaag
     3001 ccccaagcca ggagctggag ctgcagagtt ccagggcaga agggcctggt gcagaagcca
     3061 gcgtgctgga caccagagtc tccgtcgccc agctccgaaa catatttatg gagtcaaccc
     3121 gagccagcaa gaaacctgaa ctccaatccc gggttgagag gtcagctgaa ggaattggct
     3181 tgcccatgga acgagagaga gggtctcgga aaccaagacg ctatctttct cctggtgaaa
     3241 gtagaaaaac ttctgagcga tttagaactc aacctataac ctcagcagag cgaaaggagt
     3301 cggataggta cccttctggt tcagaaatac cagtggtcga ggatgaagaa aaggtggatg
     3361 agcgggccaa gctgagcgtg gctgccaaga ggctgctttt cagggaaatg gagaaatcct
     3421 ttgatgaaca cactgtccca aagagacatt cgagaaatgc agctgtggag cagaggctgc
     3481 gtcgcctgca ggaccgttca cacacacagc ccatcaccac ggaggaagtg gtcattgcag
     3541 ctactgaacc tatccctgct tcgtgttctg gggtcaccca ccctgtaacg gcgagacttc
     3601 ctagccccac tgtagctaga agcagtgtgc agcctgccag gttacaagca tctgctcacc
     3661 aaaaggcttt agccagggac caggcaaatg agggcagaga gtctgccgaa ccaggcgaac
     3721 ctgattcctc cactctgagc ttagcagaga aattggcctt gtttaacaaa ttgtcccagc
     3781 cagtctccaa agccatttcc acccgaaaca gaatagatgt gcggcagagg aggatgaacg
     3841 caaggtacca gacgcagccg gtcactcttg gagaagtgga gcaggtgcag agtgggaaac
     3901 tcatctcctt ctcccccact gtgaacacct ctgtttctat tatggcctct gcggttgctc
     3961 ccacatatgc aggggacctt cggaagctat ctgttgacaa caacacaagt gccactgact
     4021 ataagtctcc tcctgcggaa aactcagact ctccagtcag aagcattctg aaaccacaag
     4081 cttggcggcc tttggtagag cacagtggga gcaaaggaat gccgggggag tctggaaaga
     4141 cagaaagcaa gaatgctctg actgtggctg cagaagacag tggcgttcaa acacgaggag
     4201 ccttcgagga ggaagaggag ccctcctacc ccatccttgg cagagtcagg gagggtgatg
     4261 gccagaagga gcctaagcat gtcgtcctaa ggaggggaag cttagagcta ggcaatcctt
     4321 ctgctgcaca ccttggtgat gagctgaagg aagtttccac tgctaaaagc agtttgcaag
     4381 agaatctaga cctgaaggat aagcaggcct cagaggagaa cacagatgtg gaaactgtca
     4441 tgaggaagtt ctcactgaaa gagtttggag aaacgacttc tgagcagaca gaggtggctg
     4501 ccaggaaggc ctcggttcag atggcgaccc caggggcctg gaagcagcag gagtcctctg
     4561 agcaactagc agagaagctc ttcaagaacc cttgtgccat gtttgcttct ggagaggtca
     4621 aggtgccagt aggcgacagt ttcctggatt cacccagcaa aaccatgtca atcaaagaaa
     4681 ggctggcact gttaaagaag agtggcgagg aagactggaa gaacaggctg attcggaagc
     4741 aggaatatgg caaagccact ggcggcctgc acacgcagga ggtggagcag tccctgaaga
     4801 aaaagagagt cacagaaagt cgagagagcc agatgacgat tgaagagagg aagcacctca
     4861 tcactgtgcg cgaggaagcg tggaagacga agggcagagg cgctgccaat gactccacac
     4921 agttcaccgt ggcaggaaga atggtgaaga aaggtctggc atcacccaca tccataactc
     4981 ccatctcatc ccctctctgc agtaaatcaa ggggcactac cccggtttcc aaacccttgg
     5041 aagatattga agccagacca gatatgcagc tggaatcaga cctgaagttg gacagactgg
     5101 aaaccttcct aaggaggcta aataacaagg ttgctgggat acaggaaacg gttctcacgg
     5161 tcactggcaa atctgtcaag gaggtgatga agttggatga tgatgaaaca tttgccaaat
     5221 tctaccgcag cgtggatcac agtataccca gaagtcctgt ggagctggag gaggacttcg
     5281 atgtcatttt cgacccttat gctcccaagt tgacatcttc tgtggcagag cataagcgcc
     5341 aggtcaggcc caagcgccgg gtccaggctt ccaagaaccc tctcaagctg ctggcagcaa
     5401 gagacgacct ccttcaggaa tacacagagc aaaggctaaa tgtggccttc atggagtcca
     5461 agcggatgaa agtggaaaag atgtcatcca actccaactt ctcagaagtg actctggcag
     5521 gcctggccag tagggagaac ttcagcaaca tcaacctgag gagtgtcaat ctgatggagc
     5581 aaaactccaa caacagcgcg atgccctaca agaagctcat gctgttgcag attaaaggaa
     5641 gaagacatgt gcagacaagg ctggtggagc cccgagcctc atcactcaac agtggggact
     5701 gcttccttct gctgtctcct cagtactgct tcctgtgggt gggagagttc tccaatgtca
     5761 tcgagaaagc aaaggcatct gaacttgcaa cgttaattca gacaaagagg gagcttggtt
     5821 gtagagctac atacatccag accatcgaag aaggaattaa cacacatact cacgcagcca
     5881 aagacttctg gaagctcttg ggtggccaga ccagttacca gtctgccgga gacccaaagg
     5941 aagatgagct gtacgagaca gccatcatag agacgaactg tgtctaccgc ctgacagatg
     6001 acaaactcgt tcctgatgat gactactggg ggaagattcc caagtgttcc cttctgcagt
     6061 ccaaagaggt cctggtgttt gactttggta gtgaagttta cgtgtggcat gggaaggaag
     6121 tcacgttagc acaacggaaa atagcattcc agctggctaa gcacttatgg aacggaacct
     6181 ttgactatga gaactgtgac atcaaccccc tggaccccgg agagtgcaac ccgctcattc
     6241 ccaggaaagg acaaggacgg cctgactggg caatatttgg aagagttacg gagcacaatg
     6301 agactattct gttcaaagag aaattccttg actggacaga gctgaagaga cccacagaga
     6361 agaattctgg agaagttgtc cagcagaagg atgacccgag ggccgacgtc aagccttatg
     6421 atgtgacacg gatggtggcg acgccccaga ttacagctgg caccatctta gatggggtga
     6481 acgttggccg tggctatggc ctggtggaag gagatgacag aagacagttt gagattgcca
     6541 ctgtctctgt ggatgtgtgg cacattctgg aatttgacta cagcaggctc cccaggcaga
     6601 gcatcgggca gttccacgag ggggacgcct atgtggtcaa gtggaagtac atggcaagta
     6661 cagcagtggg aagccgacaa aagggagagc acctggtgag agtggctggc aaagagaagt
     6721 gtgtctattt cttctggcaa ggccggcact cgaccgtgag tgagaaggga acgtcagctc
     6781 tcatgacagt agaactggat gaagagaggg gggcccaggt ccaggtcctg cagggaaagg
     6841 agccaccctg cttccttcag tgtttccaag ggggtatggt ggtgcactcc ggacggaggg
     6901 aagaggaaga agaaaatgtg caaagtgaat ggagactgta ttgtgtgcga ggagaggtgc
     6961 ccatggaagg gaacttgcta gaagtggcct gtcattgcag cagtctgagg tccaggactt
     7021 ccatggtcgt tctgaatata aacaaggccc tcatctactt gtggcatgga tgcaaagctc
     7081 aaggtcatac gaaggaggtt ggaaggactg cagcaaataa gatcaaggaa gaatgtcccc
     7141 tggaagcagg tttgcacagc agcagcaacg tgacaataca tgagtgtgac gaaggatccg
     7201 agcccctggg attctgggac gctctgggga ggagggacag aaaggcctat gactgcatgc
     7261 ttcaagatcc tggaagtttt aacttcgcgc cccgcctgtt catcctcagc agctcctccg
     7321 gagacttctc tgcgacagag tttgtgtacc ccgcacaagc gccctctgcc gtcagctcca
     7381 tgcctttcct gcaggaggac ctgtacagcg cgccgcagcc agctctcttc cttgttgaca
     7441 accatcacga ggtgtacctc tggcaaggct ggtggcccac tgaaaacaag ataaccggct
     7501 ctgcccgcat tcgctgggcc tcagaccgga agagtgccat ggagacggtc ctgcagtact
     7561 gccgaggaaa gaatctcaaa aggccacccc ccaagtctta ccttattcat gctgggctag
     7621 agcccctgac attcaccaac atgtttccca gctgggaaca cagagaagac attgctgaaa
     7681 tcacagaaat ggatactgaa gtttccaacc agatcacctt ggtggaagat gtattagcca
     7741 agctctgtaa aaccatctat ccactggcag atctgcttgc caggccactc ccagaggggg
     7801 tcgaccctct aaagcttgag atttatctca cagatgaaga ctttgagttt gcactcgaca
     7861 tgtccagaga tgaattcaat gcactgccca cctggaagca agtgaacctg aagaagtcaa
     7921 agggcctatt ctgaccgcgg gcccgggatc caccggatct agataactga tcataatcag
     7981 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     8041 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     8101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8161 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
     8221 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     8281 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     8341 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     8401 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     8461 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     8521 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     8581 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     8641 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8761 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     8821 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8881 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8941 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9001 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9061 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9121 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     9181 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     9241 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     9301 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     9361 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     9421 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     9481 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     9541 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     9601 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     9661 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     9721 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     9781 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     9841 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     9901 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     9961 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
    10021 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    10081 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    10141 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
    10201 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
    10261 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    10321 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    10381 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
    10441 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10501 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10561 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10621 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10681 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10741 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10801 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10861 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10921 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10981 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    11041 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11101 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11161 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
//
