LOCUS       sequence-252754-        9838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K 
            with Toluic acid induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Wannier TM, Nyerges A, Kuchwara HM, Czikkely M, Balogh D, Filsinger 
            GT, Borders NC, Gregg CJ, Lajoie MJ, Rios X, Pal C, Church GM
  TITLE     Improved bacterial recombineering by parallelized protein discovery.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13689-13698. doi: 
            10.1073/pnas.2001588117. Epub 2020 May 28.
  PUBMED    32467157
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2001588117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-06-16- 16"; volume: "117"; issue: "24"; pages: 
            "13689-13698"
COMMENT     Sequence Label: pORTMAGE312B
FEATURES             Location/Qualifiers
     source          1..9838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..81
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      2838..2932
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(3053..3072)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3064..3879
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            4046..4154
                     /note="incP origin of transfer"
     CDS             complement(4165..5016)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(5003..5842)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(6353..7324)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      7431..7825
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(7863..7949)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7974..8939)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
ORIGIN
        1 agtccagcct tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc tagtaaggcc
       61 taccccttag gctttatgca acagaaacaa taataatgga gtcatgacca tgcctaggcc
      121 gcggccgcgc gaattcagaa ggagaatata ccatgggtac tgctctaacg ccgttattaa
      181 ccaagtttgc cacccgctat gagatgggaa ctacccccga agaggtcgct aatacgctga
      241 aacagacttg tttcaagggc caagtgaacg atagtcagat ggtagccctt ttgatcgttg
      301 cggatcaata taagctcaat ccattcacaa aagagctcta cgcgttccct gacaaaaata
      361 atggtattgt tccagttgtg ggagtcgatg gttgggctag aattattaac gagaatcccc
      421 agtttgatgg gatggaattc agtatggatc aacagggaac tgaatgcact tgtaaaattt
      481 accgcaaaga ccgctcgcac gccatcagcg ccaccgagta catggctgag tgcaaaagga
      541 acactcaacc ttggcagtct cacccgcgac gtatgctgcg tcataaggct atgattcaat
      601 gcgccagact agcctttggt ttcgcgggga tctacgatca ggatgaggcc gaacgcattg
      661 ttgaacgaga tgtaactccc gccgagcaat acgaggatgt atccgaagcg atttgtctga
      721 tcaaagactc accaactatg gaggacttgc aggccgcgtt ctcaaacgcg tggaaagctt
      781 acaagactaa aggtgcccgt gatcaactga ctgctgctaa agaccagaga aaaaaggagc
      841 tgttggatgc gcccattgat gtcgaattcg aagaaactgg agatgatcgt gctgcgtaag
      901 gatccaggag aatacacatg ccaattcagg tcttaccgcc acaactggcg aaccagattg
      961 ccgcaggtga ggtggtcgag cgacctgcgt cggtagtcaa agaactagtg aaaaacagcc
     1021 tcgatgcagg tgcgacgcgt atcgatattg atatcgaacg cggtggggcg aaacttatcc
     1081 gcattcgtga taacggctgc ggtatcaaaa aagatgagct ggcgctggcg ctggctcgtc
     1141 atgccaccag taaaatcgcc tctctggacg atctcgaagc cattatcagc ctgggctttc
     1201 gcggtgaggc gctggcgagt atcagttcgg tttcccgcct gacgctcact tcacgcaccg
     1261 cagaacagca ggaagcctgg caggcctatg ccgaagggcg cgatatgaac gtgacggtaa
     1321 aaccggcggc gcatcctgtg gggacgacgc tggaggtgct ggatctgttc tacaacaccc
     1381 cggcgcggcg caaattcctg cgcaccgaga aaaccgaatt taaccacatt gatgagatca
     1441 tccgccgcat tgcgctggcg cgtttcgacg tcacgatcaa cctgtcgcat aacggtaaaa
     1501 ttgtgcgtca gtaccgcgca gtgccggaag gcgggcaaaa agaacggcgc ttaggcgcga
     1561 tttgcggcac cgcttttctt gaacaagcgc tggcgattga atggcaacac ggcgatctca
     1621 cgctacgcgg ctgggtggcc gatccaaatc acaccacgcc cgcactggca gaaattcagt
     1681 attgctacgt gaacggtcgc atgatgcgcg atcgcctgat caatcacgcg atccgccagg
     1741 cctgcgaaga caaactgggg gccgatcagc aaccggcatt tgtgttgtat ctggagatcg
     1801 acccacatca ggtggacgtc aacgtgcacc ccgccaaaca cgaagtgcgt ttccatcagt
     1861 cgcgtctggt gcatgatttt atctatcagg gcgtgctgag cgtgctacaa cagcaactgg
     1921 aaacgccgct accgctggac gatgaacccc aacctgcacc gcgttccatt ccggaaaacc
     1981 gcgtggcggc ggggcgcaat cactttgcag aaccggcagc tcgtgagccg gtagctccgc
     2041 gctacactcc tgcgccagca tcaggcagtc gtccggctgc cccctggccg aatgcgcagc
     2101 caggctacca gaaacagcaa ggtgaagtgt atcgccagct tttgcaaacg cccgcgccga
     2161 tgcaaaaatt aaaagcgccg gaaccgcagg aacctgcact tgcggcgaac agtcagagtt
     2221 ttggtcgggt actgactatc gtccattccg actgtgcgtt gctggagcgc gacggcaaca
     2281 tttcactttt atccttgcca gtggcagaac gttggctgcg tcaggcacaa ttgacgccgg
     2341 gtgaagcgcc cgtttgcgcc cagccgctgc tgattccgtt gcggctaaaa gtttctgccg
     2401 aagaaaaatc ggcattagaa aaagcgcagt ctgccctggc ggaattgggt attgatttcc
     2461 agtcagatgc acagcatgtg accatcaggg cagtgccttt acccttacgc caacaaaatt
     2521 tacaaatctt gattcctgaa ctgataggct acctggcgaa gcagtccgta ttcgaacctg
     2581 gcaatattgc gcagtggatt gcacgaaatc tgatgagcga acatgcgcag tggtcaatgg
     2641 cacaggccat aaccctgctg gcggacgtgg aacggttatg tccgcaactt gtgaaaacgc
     2701 cgccgggtgg tctgttacaa tctgttgatt tacatccggc gataaaagcc ctgaaagatg
     2761 agtgatctag agtcgacctg caggcatgca agcttgcggc cgcgtcgtga ctgggaaaac
     2821 cctggcgact agtcttggac tcctgttgat agatccagta atgacctcag aactccatct
     2881 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt
     2941 ccccaataat tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat
     3001 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     3061 gttatgagcc atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg
     3121 gatgcggatc tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc
     3181 atttatcgtc tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc
     3241 agcgtggcga acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg
     3301 ccgctgccga ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc
     3361 gcgattccgg gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac
     3421 attgtggatg cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc
     3481 ccgtttaaca gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc
     3541 ctggtggatg cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg
     3601 aaagaaatgc ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt
     3661 agcctggata acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt
     3721 gtgggcattg cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt
     3781 agcccgagcc tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac
     3841 aaactgcaat ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcc
     3901 gcgcgttgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     3961 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     4021 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     4081 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     4141 tgcgaggctg gccgtaggcc ggcctcagcc tgccgccttg ggccgggtga tgtcgtactt
     4201 gcccgccgcg aactcggtta ccgtccagcc cagcgcgacc agctccggca acgcctcgcg
     4261 cacccgctgg cggcgcttgc gcatggtcga accactggcc tctgacggcc agacatagcc
     4321 gcacaaggta tctatggaag ccttgccggt tttgccgggg tcgatccagc cacacagccg
     4381 ctggtgcagc aggcgggcgg tttcgctgtc cagcgcccgc acctcgtcca tgctgatgcg
     4441 cacatgctgg ccgccaccca tgacggcctg cgcgatcaag gggttcaggg ccacgtacag
     4501 gcgcccgtcc gcctcgtcgc tggcgtactc cgacagcagc cgaaacccct gccgcttgcg
     4561 gccattctgg gcgatgatgg ataccttcca aaggcgctcg atgcagtcct gtatgtgctt
     4621 gagcgcccca ccactatcga cctctgcccc gatttccttt gccagcgccc gatagctacc
     4681 tttgaccacc atggcatcag cggtgacggc ctcccacttg ggttccagga acagccggag
     4741 ctgccgtccg ccttcggtct tgggttccgg gccaagcact aggccattag gcccagccat
     4801 ggccaccagc ccttgcagga tgcgcagatc atcagcgccc agcggctccg ggccgctgaa
     4861 ctcgatccgc ttgccgtcgc cgtagtcata cgtcacgtcc agcttgctgc gcttgcgctc
     4921 gccccgcttg agggcacgga acaggccggg ggccagacag tgcgccgggt cgtgccggac
     4981 gtggctgagg ctgtgcttgt tcttaggctt caccacgggg cacccccttg ctcttgcgct
     5041 gcctctccag cacggcgggc ttgagcaccc cgccgtcatg ccgcctgaac caccgatcag
     5101 cgaacggtgc gccatagttg gccttgctca caccgaagcg gacgaagaac cggcgctggt
     5161 cgtcgtccac accccattcc tcggcctcgg cgctggtcat gctcgacagg taggactgcc
     5221 agcggatgtt atcgaccagt accgagctgc cccggctggc ctgctgctgg tcgcctgcgc
     5281 ccatcatggc cgcgcccttg ctggcatggt gcaggaacac gatagagcac ccggtatcgg
     5341 cggcgatggc ctccatgcga ccgatgacct gggccatggg gccgctggcg ttttcttcct
     5401 cgatgtggaa ccggcgcagc gtgtccagca ccatcaggcg gcggccctcg gcggcgcgct
     5461 tgaggccgtc gaaccactcc ggggccatga tgttgggcag gctgccgatc agcggctgga
     5521 tcagcaggcc gtcagccacg gcttgccgtt cctcggcgct gaggtgcgcc ccaagggcgt
     5581 gcaggcggtg atgaatggcg gtgggcgggt cttcggcggg caggtagatc accgggccgg
     5641 tgggcagttc gcccacctcc agcagatccg gcccgcctgc aatctgtgcg gccagttgca
     5701 gggccagcat ggatttaccg gcaccaccgg gcgacaccag cgccccgacc gtaccggcca
     5761 ccatgttggg caaaacgtag tccagcggtg gcggcgctgc tgcgaacgcc tccagaatat
     5821 tgataggctt atgggtagcc attgattgcc tcctttgcag gcagttggtg gttaggcgct
     5881 ggcggggtca ctacccccgc cctgcgccgc tctgagttct tccaggcact cgcgcagcgc
     5941 ctcgtattcg tcgtcggtca gccagaactt gcgctgacgc atccctttgg ccttcatgcg
     6001 ctcggcatat cgcgcttggc gtacagcgtc agggctggcc agcaggtcgc cggtctgctt
     6061 gtccttttgg tctttcatat cagtcaccga gaaacttgcc ggggccgaaa ggcttgtctt
     6121 cgcggaacaa ggacaaggtg cagccgtcaa ggttaaggct ggccatatca gcgactgaaa
     6181 agcggccagc ctcggccttg tttgacgtat aaccaaagcc accgggcaac caatagccct
     6241 tgtcactttt gatcaggtag accgaccctg aagcgctttt ttcgtattcc ataaaacccc
     6301 cttctgtgcg tgagtactca tagtataaca ggcgtgagta ccaacgcaag cactacatgc
     6361 tgaaatctgg cccgcccctg tccatgcctc gctggcgggg tgccggtgcc cgtgccagct
     6421 cggcccgcgc aagctggacg ctgggcagac ccatgacctt gctgacggtg cgctcgatgt
     6481 aatccgcttc gtggccgggc ttgcgctctg ccagcgctgg gctggcctcg gccatggcct
     6541 tgccgatttc ctcggcactg cggccccggc tggccagctt ctgcgcggcg ataaagtcgc
     6601 acttgctgag gtcatcaccg aagcgcttga ccagcccggc catctcgctg cggtactcgt
     6661 ccagcgccgt gcgccggtgg cggctaagct gccgctcggg cagttcgagg ctggccagcc
     6721 tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg ctggccagcc tgctgcacca
     6781 gcgccgggcc agcggtggcg gtcttgccct tggattcacg cagcagcacc cacggctgat
     6841 aaccggcgcg ggtggtgtgc ttgtccttgc ggttggtgaa gcccgccaag cggccatagt
     6901 ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct ggccagcgtc cgggcaatct
     6961 gcccccgaag ttcaccgcct gcggcgtcgg ccaccttgac ccatgcctga tagttcttcg
     7021 ggctggtttc cactaccagg gcaggctccc ggccctcggc tttcatgtca tccaggtcaa
     7081 actcgctgag gtcgtccacc agcaccagac catgccgctc ctgctcggcg ggcctgatat
     7141 acacgtcatt gccctgggca ttcatccgct tgagccatgg cgtgttctgg agcacttcgg
     7201 cggctgacca ttcccggttc atcatctggc cggtggtggc gtccctgacg ccgatatcga
     7261 agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct
     7321 tcatcgggcc accaagcgat tcccacacat tatacgagcc ggaagcataa agtgtagcta
     7381 gatccgaagg atgagccggg ctgaatgatc gaccgagaca ggccctgcgg ggctgcacac
     7441 gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc gctccagcgt
     7501 atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc cctgccgcgc
     7561 agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg cctctggccg
     7621 ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg cctagtggat
     7681 tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg cccctgctgg
     7741 gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta ttgcaggggg
     7801 gcgtgacagt tattgcaggg gttcgtgaca gttagggcgc gcccagctgt ctagggcggc
     7861 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     7921 tctttcgact gagcctttcg ttttatttga tgcctttaat taaacgttcg taatcaagcc
     7981 acttcctttt tgcattgacg cagggtgtcg gaaggcaact cgccgaacgc gctcctatag
     8041 ttttcagcga agcgtcccaa atgtaagaag ccgtagtcta gggctatctc agttatacta
     8101 cgcacattgg cactgggatc gttcaagcag gcgcggatgc tttcgagctt gcggttgcgg
     8161 atgtagttct tcggcgtggt gccggcgtgc ttctcgaaca aattgtagag cgagcgtgga
     8221 ctcatcatcg ccagctccgc taaccgctca aggctgatat tccgtttgag attctcctca
     8281 atgaattgaa cgactcgctc gaaagacggg ttacctttgc tgaaaatttc acggctgaca
     8341 ttgctgccca gcatttcgag cagcttggaa gcgatgatcc ccgcatagtg ctcttggacc
     8401 cgaggcatcg actttgtatg ttccgcttcg tcacaaacta acccgagtag attgataaag
     8461 ccatcgagtt gctggagatt gtgtcgcgcg gcgaaacgga taccctccct cggcttgtgc
     8521 caattgttgt cactgcacgc ccgatcaagg accactgagg gcaatttaac gataaatttc
     8581 tcgcaatctt ctgaataggt caggtcggct tggtcatccg gattgagcag caatagttcg
     8641 cccggcgcaa aatagtgctc ctggccatgg ccacgccaca ggcaatggcc tttgagtatt
     8701 atttgcagat gataacaggt ctctaatcca ggcgagatta ccctcacgct accgccgtag
     8761 ctgattcgac acagatcgag gcatccgaag attctgtggt gcagcctgcc tgccgggcgc
     8821 ccgcccttgg gcaggcgaat agagtgcgta ccgacatact ggttaacata atcggagact
     8881 gcatagggct cggcgtggac gaagatctga cttttctcgt tcaataagca aaaatccata
     8941 gttcacggtt ctcttatttt aatgtgggct gcttggtgtg atgtagaaag gcgccaagtc
     9001 gatgaaaatg catctcgacg tgatgcgtat acgggttacc cccattgcca cgttgcgcca
     9061 tcctttttgc aatcagtgac cacttttcca agcaaaaata acgccaagca gaacgaagac
     9121 gttcttttta agaagcgaga acaccagaag ttcgtgctgt cggggcatgg ggcgacgaat
     9181 tggcggataa aggggatctg ctggatatta cggccttttt aaagaccgta aagaaaaata
     9241 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgt
     9301 aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     9361 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     9421 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     9481 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     9541 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     9601 ccatgcatgg gaattagctt gatctgacca acgaccggta gcggagctat ccaacggcgg
     9661 tataccagga aaacacacag caggtacatc agaacagtac catgactgaa gaacaaatag
     9721 ttttttcctg atccataaag cagaacggcc tgctccatga caaatctggc tccccaacta
     9781 atgccccatg cagccagcat aaccagcata aagtgcagtg tccggtttga tagggata
//
