LOCUS       sequence-252757-       11980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Matthews BJ, Younger MA, Vosshall LB
  TITLE     The ion channel ppk301 controls freshwater egg-laying in the 
            mosquito Aedes aegypti.
  JOURNAL   eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963.
  PUBMED    31112133
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-05-21"; volume: "8. pii: 43963. doi"; pages: " 
            10.7554/eLife.43963"
COMMENT     Sequence Label: Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
FEATURES             Location/Qualifiers
     source          1..11980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3887..3906)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3888..3906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3913..3930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3913..3929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3922..3944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4070..4525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4157..4176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4367..4388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4592..4611)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4711..4733
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4771..4789)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4857..4961
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5147..5525
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(5181..5200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5994..6582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6483..6502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6736..6753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6870..6891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6906..6911,6912..6929,6930..6936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6944..6960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6949..6971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6968..6984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6968..6984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7005..7023
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             9593..10258
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             10265..10978
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(10295..10323)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10918..10938
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             10982..11035
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11036..11710
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(11216..11236)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     11628..11651
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             11720..11740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11859..11980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11896..11915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11950..11969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcgtcgacgt caaaatcacc acttttgaag cgttgaaacc accgttcaca cgttttcaca
       61 gttggtactt gttcgccaaa ggcttcaaca agcattcggt gcgattccgc agctgttttc
      121 ttcaaatgaa aacagaaaat taatactgtc cgcgtttgct ctttattcgg cacgaaactc
      181 gacatgttga ctgcactgag agtaaacaat tatgacgctc aattcgcgcc aaactatggt
      241 ggttcgacag tcaaggttga cacttcacaa ggtcaaagtt ttatgacaat cgataaatat
      301 ttacgtttgc gagacatcta tatgttcgaa ccgacattcc ctacttgtac acctggtaaa
      361 aaattaaaac taggtccccg ttcttacgca tatatacaca ggtacgtggg tgttaattaa
      421 ttactttaaa acattaagga acttcgattg gcatttaaca aggaaactaa catcatattg
      481 ttatacaaat aaaagttgct catttcttac tcgtaattcg aaacctttct cggttctttt
      541 ttttaccata ctatcacttt aaagtggttt taaccaacat gcatacattt cgtttttttt
      601 tcgttttact cgtctgcgtt cgctctttgt tttttaacca aagtaaatct gactcaggga
      661 agtcactcaa aacatgtttg cctgagctct cgtattccaa atggaactcc gggaaaaata
      721 tatgctcaaa agtggctcaa aactgtatct attcgtattt tgatatacac aataataaaa
      781 ctgctcgaag aactccaatc tgttacacaa tgcctggtca ttgtttacat ttagctcact
      841 tgtgtactta tgtgcaccca tacacaccca tgtgtagata aaggatggct tatcttatca
      901 ctgacaaaac agcggagcat gcaagaatgc tgtttgtctt tgtccattgg cttcgattaa
      961 gtagtcgaaa aggaaaacgg taaatggaat gcataacttt gccctctcac tccctctaat
     1021 cggacggaag agtcggacgt tatgacctgt cctgatttgg agaaatcttt tctatatttc
     1081 atttatttcg cttacttccc tacttctttc aaatgcacta atgcgaaaaa cattattttt
     1141 ttcaaattaa atggtcaagt gtgcttctaa tgaaatccat agtctcaata catacatata
     1201 tgcagtcggc aaaaaaggag gcacatactc gtagaaattt ttcattgctc tgtatatgga
     1261 tttgtaaatg gtataaaaag catatatcag tatcagttct cttcccattc ccgctctcag
     1321 tgcgctctct cggttgagca gttttcgagc ctctcgagtt cgagttcacc gagttcgagt
     1381 tgcgacggtt cgcccactca gtttgcccac agctctgaat gcgagcgtaa cgatcgcaag
     1441 ccgcctgtct tgcagtgaag tgcattttct caagccgctt cctcgatttg tatttggttg
     1501 gacaacagcc cataacttga acccgtcttt aatgcccgta tctctgcgag agtgagtttc
     1561 caatggcgat ttgccgagtg cgcgtgtgca gggcaccctt tccttcctgt tgtcgtccgg
     1621 agagtattcg cgttgagtgg aacagccgta taagagtggt tgggctagcg gatttaaagg
     1681 aatgtgggtg ctgtgccttt aagttaggac tccttgtgaa gggatacgca gagcagcggc
     1741 tggatcgcaa gttatttcta aaaatatatg gaaagtaatg ggggagatat acgaaatgta
     1801 tctgcacgaa aactcatatg ctcgccagat gtttagcttg agcaaaaact tcagattcct
     1861 taaaatttcc gttcttatta tttattattt ctgctttagc gcaggtttta atcagaactt
     1921 ttttccctta atataattgg atgtgtgtat ttgcgcagtg ttcggctctc ccagattttc
     1981 tggtttttaa ttttttccct tttctttttt aataagaaag ttttgaagtt tgattacatt
     2041 ctttcctata tctttcgatt ctataaactt tacatattgg tctagacgcg tggttccatc
     2101 caattgagag tgaactattc gatttcttat attaagttgc tcttccggtg gctcagacac
     2161 agaagttgag aaacgagaag ttttaaaaca cacactatcc ggacaactag acatactttc
     2221 gaaatctgct gtttgccgtt ttttgcatag gaaggctgtt tgttttttgg tcaaattgta
     2281 aaaatatttg cttttcttta ggtgggcttt ggacttcgct ttttcgttgg caataactat
     2341 ttttgtaaga cttctggaca tctttgtcct gtagttatcg aaattaatcg gggtgcgcaa
     2401 caagatgtga taacctgcgt tcgtctgaaa aatctcagat agttcgccat cattcagcaa
     2461 caaaatgttt ctctcaaaga cgaaagacgt ttgtgtaagg ctcaaggggn ccaagtcacc
     2521 gccatgccgg gcggagcagc agtcggagat catttttgcc cattcagcaa actcacattt
     2581 gcctgattga attagatccc tcgcatgcat tatcttatta ccgcttcctg cctcgttctc
     2641 cttaccaccc gttcacgaaa tgaactacac ctggtccgaa ttcgtggggt ttcaccaaga
     2701 agtggcttga agggaagcgg gcatttgtca ttggccccca gggaaatccc caaaaaacgg
     2761 gccccccagg attccatttt ctttttccgg gaggtaaaga gcctccccgt ttccgatatg
     2821 tactacacct gtccgattcg ttgtgcttca ccaggatgtg tctgcagcgg agcctttatt
     2881 tgtcattgca ctcagggtag tccccaagaa cggcgcccca ggcattccta ttcttttctc
     2941 ggtaggggct tggaggcagt gtgaaatata ccttcctggt aattgtgtcg tagaagtagc
     3001 actccttggt actgtgaaca attcgctctt cccatccaag gggcagcttg gttaggcctc
     3061 tcagaattat gacgttcgcc agttaggatc gagagcccac tgtccgcatc accataggag
     3121 tcgcttttaa cgttatcact cgaatattta cttttagagc tcgcgtctaa ccaaccccaa
     3181 gagtggtcac aagtttgctg atcttcattt tggccatctt ctattttaaa tatatcaaac
     3241 ttgcatagcg ccggcttcat ggtgttcatg tttgcacaaa ccagacggta taggtattta
     3301 tgtgttaagt atagatactg aataggttac tgctttgagc caaactctta aagaaaaata
     3361 aagcaatgca atcttgtttg ttttacagct cggctttaag gcatatattt ttaattatgt
     3421 tttattttca tgcttttatt tttttttagg tttccgaaat agttatgaat cttatactga
     3481 ctgtaaccac ggctctgcac ctagttttgg tggtattggc gcacaatccg ccgtcgattc
     3541 gtggcgaaag ccattctgat ggcattcgtc tggtcatgga aaagaggtcg ggcgatgtag
     3601 tacacattcg tccagctcgg ggcaccctgg gagacgaaac agtcgcatca ccatctccaa
     3661 caaaaagtga cacccaccgc accgcgaata atcgtaagcc gaacaagccg caagagccct
     3721 ctcagcaggt ggggccccac aagtcgggat ctagaaaact agtggtttgc gaaaaatggc
     3781 ggggcactgt catcacagct accacaacca aatcacaaac aaggccacaa gaacactgat
     3841 aagcaacaac gaggaggagg atccccgggt accgagctcg aattcgccct atagtgagtc
     3901 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3961 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4021 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4501 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     4561 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4621 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4681 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4741 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4801 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4861 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4921 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4981 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5041 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc cacgagtggg ttacatcgaa
     5101 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5161 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5221 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5281 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5341 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5401 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5461 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5521 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5581 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5641 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5701 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5761 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5821 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5881 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5941 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6001 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6061 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6121 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6181 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6241 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6361 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6661 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6721 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6781 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6841 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6901 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6961 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
     7021 agggaacaaa agctttatgg tttatgatta tcaatttcgt tcccattgct ggaatatctt
     7081 tcgggttgtt tgttatgcct cgcttttcac tgcgtgctag cagttccctt ttccttagga
     7141 atgaccatgc gcacttccac tttcgtggga taaaaatggt aaatccggct ttttcttaaa
     7201 gctaccaaat gatgtagata ggcatcccac agtacgggct ccagtcggtg aatatagaaa
     7261 ctatcaggtg tacaagtatg aaatgtcgtt ttttttaaat caaaaaacac gttaaatttt
     7321 gtggaaaaag aaaaaatcat ttattcaaag tatttgccgt cgctagctac acatttttcc
     7381 catctctcgg gcaatttgtg gattccacgc cagtagaact catcgtcttt tgcggcgaac
     7441 cattcatcga gccatttttt cacactttcg taagaatcga agcgctgctc agcgagtgcg
     7501 tgtcccatcg aagcgaatag gtggtaatcg gatggggcca ggtctggtga gtaagccgca
     7561 tgcggaagca cttcccaatt gagtgtttcc aacgtgtcgc gaaccgctct tgccgtatgt
     7621 gatggagcgt tgtcatggag aaaaatgacc ctgtgttgtc ttttttgata ttccggtcgt
     7681 tttctctgaa gcgcacggtt caaattgatc aattgttgtt ggtagcgtgc cgtattcacc
     7741 gtttcgccgg gtttcaagag ctcatagtaa atgacaccgc tctgatccca ccaaacacag
     7801 agcatcgtct tcttgccaaa gcgattcggt cgagcagtcg atgtggccgg ttgtccagga
     7861 tcaacgtatg actttttacg tttaggatta acaaaaaaga tccatttttc atctccagta
     7921 acgatacgat gcaaaaacga cttccttttg tatcgtgaaa gcaaaatttc gcatgtgttt
     7981 ttgcgcctct ccatctgcct ctcgttcaac tcatgtggca cccatctacc gaccttctga
     8041 atctttccca tctctcgcaa gcgattggaa actgcttgtt gacttacttc caactgctct
     8101 gcgagttgtt tttgcgtttg agcatcgtct tcatccaata atgcttgcag ttcggcgtct
     8161 tcgtaccttt ttggcggttt tccgtgctct ttgtcgtcga tatctttaca tgtagcttgt
     8221 gcattgaatc caattataat ttgccttggc accagctgag ccagacaaga aagaaagctt
     8281 cccagaagta tatcgattta gaagggttga cgtcacttgc tgactgcact aatacagcaa
     8341 atgatgcaat tagaatgatt caagtgaaat tcccaaatta ctgatttttc tctggatttg
     8401 gttatcagat tacattcgaa gctaagatta gctaccgaaa ttgtcgatca aatcaggaaa
     8461 tcctttctct atcgaaaaag gcattcgcac atcttcctct ctatgccata tacacgaagg
     8521 gtaggtacat tgacgtcttt gccagaagtt gaactgcatc gttcaaggta cagaatgaac
     8581 gactaacaga cacaagcacg ttttgctgtc cattcagaca cagggatggt acccatattc
     8641 gatcgatata gagccatcca accgaacaga ggtatatgta tgaatgtatt gctgaaattt
     8701 tctagaagta caaccaccac tacgacagtg tctataaaac gcccctgcaa aggcgaaacc
     8761 agctcaatcg aatacgtttc ctagtggagt gaacattacg cggcccaagt aagcagtgcc
     8821 agtgcaagtg aagtgaagtc tctagtgaaa aagagtgatc caattagcca gaggagaaaa
     8881 tttcagagtg aacaaagctt tattcaaagg acaattacta ttaaattggt gaaagtgcat
     8941 ttcggtgaag ggaatcttct agtgaaggta ggtaaattaa ttgatgaaat tatagctatg
     9001 agcgaaaact agtttggtga atgattcctt tgtctttgaa tgagcaaact attttccaag
     9061 atggcgacta ttgagctttg agtgattagt gaaaatttgc aacgcagttt catcatcatt
     9121 gataaaaccc aattgtgatt cacagcgata atcatatttc gttgaatcat cgctactaat
     9181 tgaattaaat ttctagaata ataagaataa cgtatttgct ccgtcacata tctaaaataa
     9241 atattttgat ggtaattacc cattaaggta atattaacac atatcgagaa aaaccttgag
     9301 gaaatcgtga aaacttgaag atacgcaatt tcaaaactac gtagttcaaa gtcgaaaaca
     9361 agttaatttt tcacttaaaa gtagggcgtt gttgtgacgt catcaccttc aagtgtatat
     9421 ttttcacttg gcctgcgact gcaaacgcag acaaagcaaa acaagtttaa aacctgtcgt
     9481 gtcgtgctcg aagccaaagg caatgaatca atatcaaatg agagtttgca tttcacaacc
     9541 aattactcaa gcgtttcctc gtttcttttt ctgctcaaca gagatttcaa caatggcctc
     9601 accgttgacc cgctttctgt cgctgaacct gctgctgctg ggtgagtcga ttatcctggg
     9661 gagtggagaa gctaagccac aggcacccga actccgaatc tttccaaaga aaatggacgc
     9721 cgaacttggt cagaaggtgg acctggtatg tgaagtgttg gggtccgttt cgcaaggatg
     9781 ctcttggctc ttccagaact ccagctccaa actcccccag cccaccttcg ttgtctatat
     9841 ggcttcatcc cacaacaaga taacgtggga cgagaagctg aattcgtcga aactgttttc
     9901 tgccatgagg gacacgaata ataagtacgt tctcaccctg aacaagttca gcaaggaaaa
     9961 cgaaggctac tatttctgct cagtcatcag caactcggtg atgtacttca gttctgtcgt
    10021 gccagtcctt cagaaagtga actctactac taccaagcca gtgctgcgaa ctccctcacc
    10081 tgtgcaccct accgggacat ctcagcccca gagaccagaa gattgtcggc cccgtggctc
    10141 agtgaagggg accggattgg acttcgcctg tgatatttac atctgggcac ccttggccgg
    10201 aatctgcgtg gcccttctgc tgtccttgat catcactctc atctgctacc acagccgcgg
    10261 atccatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
    10321 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg acgcaacata
    10381 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
    10441 acttgtcact actttaactt atggtgttca atgcttttca agatacccag atcatatgaa
    10501 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtccaggaaa gaactatatt
    10561 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
    10621 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
    10681 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
    10741 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
    10801 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
    10861 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
    10921 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaagg
    10981 agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatggc
    11041 ctcctccgag aacgtcatca ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt
    11101 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa
    11161 caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
    11221 cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta
    11281 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
    11341 cggcgtggcg accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt
    11401 gaagttcatc ggcgtgaact tcccctccga cggccccgtg atgcagaaga agaccatggg
    11461 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca
    11521 caaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat
    11581 ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gacgccaagc tggacatcac
    11641 ctcccacaac gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca
    11701 cctgttcctg agatctcgac ccaagaaaaa gcggaaggtg gaggacccgt aggatcataa
    11761 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    11821 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    11881 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    11941 attctagttg tggtttgtcc aaactcatca atgtatctta
//
