LOCUS       sequence-252759-        8747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s 
            calcium indicator, separated by T2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Matthews BJ, Younger MA, Vosshall LB
  TITLE     The ion channel ppk301 controls freshwater egg-laying in the 
            mosquito Aedes aegypti.
  JOURNAL   eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963.
  PUBMED    31112133
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-05-21"; volume: "8. pii: 43963. doi"; pages: " 
            10.7554/eLife.43963"
COMMENT     Sequence Label: 15x-QUAS-dTomato-T2A-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..8747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   254..288
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(779..795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(779..795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(792..814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    803..819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(827..833,834..851,852..857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    872..893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1010..1027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1181..1769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1261..1280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1940..2731,2732..2800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2563..2582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2801..2905)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2931..3386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3068..3089)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3280..3299
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3513..3535
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3527..3544
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3528..3544
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3674..3736
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      4222..4231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4228..4230,4231..4233,4234..4947)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    5070..5191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5603..5841
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5788..5809
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5917..6618
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6050..6068)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6318..6337
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6512..6535
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6622..6675
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6676..6684,6685..6702,6703..6705,6706..6738,6739..6741,
                     6742..6765,6766..6792,6793..6849,6850..6855,6856..7125,
                     7126..7143,7144..7578,7579..7584,7585..8028)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      6772..6781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8105..8170
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8300..8320
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8592..8726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
       61 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
      121 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
      181 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
      241 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
      301 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
      361 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
      421 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
      481 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
      541 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
      601 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
      661 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
      721 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
      781 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      841 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      901 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      961 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1621 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2521 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     2941 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3001 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3061 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3121 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3181 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3241 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3301 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3361 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     3421 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3481 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3541 cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc agactcgcgg
     3601 tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc tgcagaacac
     3661 gcagctagat taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgt
     3721 gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat
     3781 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
     3841 atttatgttt atttatttat taaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3901 aaaactttta tcgaattcct gcagcccggg ggatccacta gttctagtgt tcccacaatg
     3961 gttaattcga gctcgcccgg ggatctaatt caattagaga ctaattcaat tagagctaat
     4021 tcaattagga tccaagctta tcgatttcga accctcgacc gccggagtat aaatagaggc
     4081 gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga
     4141 aagctaagca aataaacaag cgcagctgaa caagctaaac aatcggggta ccgctagagt
     4201 cgacggtacg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     4261 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4321 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4381 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
     4441 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4501 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4561 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4621 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc
     4681 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     4741 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4801 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4861 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4921 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     4981 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5041 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5101 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5161 gtggtttgtc caaactcatc aatgtatctt aaagcttatc gatacgcgta cggcactaga
     5221 gcggccgcca ccgcggtcac gtgtcactgg gtcagtgggt aatcgcttat cctcggataa
     5281 acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc tcgggtaatc
     5341 gcttatcctt gggtaatcgc ttatcctcgg ataaacaatt atcctcacgg gtaatcgctt
     5401 atccgctcgg gtaatcgctt atcctcgggt aatcgcttat ccttgggtaa tcgcttatcc
     5461 tcggataaac aattatcctc acgggtaatc gcttatccgc tcgggtaatc gcttatcctc
     5521 gggtaatcgc ttatcctttc acgttgggac tcagtgagga ggacctgaat tcctgcagcc
     5581 cgagcggaga ctctagcgag cgccggagta taaatagagg cgcttcgtct acggagcgac
     5641 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa
     5701 gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa
     5761 agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt
     5821 gaatacaaga agagaactct gaatagggaa ttgggaattc gttaacagat cgggggatca
     5881 attcgttaac agatctgcgg ccgcggctcg agcaaaatgg tgagcaaggg cgaggaggtc
     5941 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     6001 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     6061 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     6121 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     6181 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     6241 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     6301 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     6361 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     6421 aaggacggcg gccgctacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     6481 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     6541 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
     6601 atggacgagc tgtacaaggg agagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6661 gagaatcccg gccctatggg ttctcatcat catcatcatc atggtatggc tagcatgact
     6721 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg
     6781 gtcgactcat cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg
     6841 ctgagctcac tcgagaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
     6901 aacttccaca tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
     6961 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
     7021 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     7081 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
     7141 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     7201 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     7261 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     7321 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     7381 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     7441 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     7501 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     7561 cacaagctgg agtacaacct gccggaccaa ctgactgaag agcagatcgc agaatttaaa
     7621 gaggctttct ccctatttga caaggacggg gatgggacaa taacaaccaa ggagctgggg
     7681 acggtgatgc ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat
     7741 gaagtagatg ccgacggtga cggcacaatc gacttccctg agttcctgac aatgatggca
     7801 agaaaaatga aatacaggga cacggaagaa gaaattagag aagcgttcgg tgtgtttgat
     7861 aaggatggca atggctacat cagtgcagca gagcttcgcc acgtgatgac aaaccttgga
     7921 gagaagttaa cagatgaaga ggttgatgaa atgatcaggg aagcagacat cgatggggat
     7981 ggtcaggtaa actacgaaga gtttgtacaa atgatgacag cgaagtgaga tctttgtgaa
     8041 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     8101 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     8161 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     8221 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     8281 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     8341 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     8401 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     8461 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     8521 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8581 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8701 tcaatgtatc ttatcatgtc tggatcgata tctataacaa gaaaata
//
