LOCUS       sequence-252781-        4233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level0 Switchgrass PvUBI1 Pol II promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH40
FEATURES             Location/Qualifiers
     source          1..4233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(2942..2953)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggagcac gtcagtgttt ggtttccact agcacgagta gcgcaatcag
     2281 aaaattttca atgcatgaag tactaaacga agtttattta gaaatttttt taagaaatga
     2341 gtgtaatttt ttgcgacgaa tttaatgaca ataattaatc gatgattgcc tacagtaatg
     2401 ctacagtaac caacctctaa tcatgcgtcg aatgcgtcat tagattcgtc tcgcaaaata
     2461 gcacaagaat tatgaaatta attttacaaa ctatttttat ttaatactaa taattaactg
     2521 tcaaagtttg tgctactcgc aagagtagcg cgaaccaaac acggcctgga ggagcacggt
     2581 aacggcgtcg acaaactaac ggccaccacc cgccaacgca aaggagacgg atgagagttg
     2641 acttcttgac ggttctccac ccctctgtct ctctgtcact gggccctggg tccccctctc
     2701 gaaagttcct ctggccgaaa ttgcgcggcg gagacgaggc gggcggaacc gtcacggcag
     2761 aggattcctt ccccaccctg cctggcccgg ccatatataa acagccaccg cccctccccg
     2821 ttccccatcg cgtctcgtct cgtgttgttc ccagaacaca accaaaatcc aaatcctcct
     2881 cctcctcccg agcctcgtcg atccctcacc cgcttcaagg tacggcgatc ctcctctccc
     2941 ttctcccctc gatcgattat gcgtgttccg tttccgtttc cgatcgagcg aatcgatggt
     3001 taggacccat gggggaccca tggggtgtcg tgtggtggtc tggtttgatc cgcgatattt
     3061 ctccgttcgt agtgtagatc tgatcgaatc cctggtgaaa tcgttgatcg tgctattcgt
     3121 gtgagggttc ttaggtttgg agttgtggag gtagttctga tcggtttgta ggtgagattt
     3181 tccccatgat tttgcttggc tcgtttgtct tggttagatt agatctgccc gcattttgtt
     3241 cgatatttct gatgcagata tgatgaataa tttcgtcctt gtatcccgcg tccgtatgtg
     3301 tattaagttt gcaggtccta gttaggtttt tcctactgat ttgtcttatc cattctgttt
     3361 agcttgcaag gtttggtaat ggtccggcat gtttgtctct atagattaga gtagaataag
     3421 attatctcaa caagctgttg gcttatcaat tttggatctg catgtgtttc gcatctatat
     3481 ctttgcaatt aagatggtag atggacatat gctcctgttg agttgatgtt gtacctttta
     3541 cctgaggtct gaggaacatg catcctcctg ctactttgtg cttatacaga tcatcaagat
     3601 tatgtagcta atattcgatc agtttctagt atctacatgg taaacttgca tgcacttgct
     3661 acttattttt gatatacttg gatgataaca tatgctgctg gttgattcct acctacatga
     3721 tgaacatttt acaggccatt agtgtctgtc tgtatgtgtt gttcctgttt gcttcagtct
     3781 atttctgttt cattcctagt ttattggttc tctgctagat acttaccctg ctgggcttag
     3841 ttatcatctt atctcgaatg cattttcatg tttatagatg aatatacact cagataggtg
     3901 tagatgtatg ctactgtttc tctacgttgc tgtaggtttt acctgtggca actgcatact
     3961 cctgttgctt cgctagatat gtatgtgctt atatagatta agatatgtgt gatggttctt
     4021 tagtatatct gatgatcatg tatgctcttt taacttcttg ctacacttgg taacatgctg
     4081 tgatgctgtt tgttgattct gtagcactac caatgatgac cttatctctc tttgtatatg
     4141 atgtttctgt ttgtttgagg cttgtgttac tgctagttac ttaccctgtt gcctggctaa
     4201 tcttctgcag atcctgcaat gtgagaccac gaa
//
