LOCUS       sequence-252795-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Sabourin LA, Rudnicki MA
  TITLE     The molecular regulation of myogenesis.
  JOURNAL   Clin Genet. 2000 Jan . 57(1):16-25.
  PUBMED    10733231
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin Genet.
            2000 Jan . 57(1):16-25."
COMMENT     Sequence Label: EMSV-Myf5 (puro)
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    2764..2898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2788..2807)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2842..2861
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3579..4178)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(4218..4717)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             complement(5272..5871)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(5911..6410)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6469..6488)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6470..6488)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6495..6512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6495..6511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6504..6526)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6652..7107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6739..6758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6949..6970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7174..7193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7293..7315
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7353..7371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7439..7543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7544..7612,7613..8404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7762..7781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8575..9163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9064..9083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9317..9334
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagcttggaa aagttcagat
      181 caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
      241 tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
      301 tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
      361 cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
      421 aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
      481 cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
      541 cgatagactg cgtcgcccca gatccccggg cgagctcgaa ttcgggccag agagagccgg
      601 aagccccagg agctctactc ggtgaagatt ttacaacgtg tgccgcttct gtttttcctg
      661 taacctcagg gggaaaaaac gaccaccaag aaacacagtg gggaagaaag cagagcaaag
      721 cacgccagag agacagtccc aaactccggg agctccgcct ggatttgctg gcctgcagca
      781 gccagggact ggcgtgtctc cctctctgct gaatccaggt attcccacct gcttctctga
      841 aggatggaca tgacggacgg ctgccagttc tccccttctg agtacttcta tgaaggctcc
      901 tgtatcccct caccagagga tgagtttggg gaccagtttg agccaagagt agcagccttc
      961 ggagcacaca aagctgagct gcagggctca gacgatgagg agcacgtgcg tgcacctacc
     1021 ggccaccacc aggctggcca ctgcctcatg tgggcctgca aagcttgcaa gaggaagtcc
     1081 actaccatgg atcggcgcaa ggccgccacc atgcgcgagc gtagacgcct gaagaaggtc
     1141 aaccaagctt tcgagacgct caagaggtgc accaccacca accctaacca gagactcccc
     1201 aaggtggaga tcctcaggaa tgccatccgc tacattgaga gcctccagga gctgctgagg
     1261 gaacaggtgg agaactatta cagcctgccg ggacagagct gctctgagcc caccagcccc
     1321 acctccaact gctctgacgg catgcctgaa tgtaacagcc ctgtctggtc ccgaaagaac
     1381 agcagctttg acagcatcta ctgtcctgat gtatcaaatg catgtgctgc agataaaagc
     1441 tccgtgtcca gcttggattg cttgtccagc attgtggatc ggatcacgtc tacagagcca
     1501 tctgagctgg ctcttcagga cacagcttcc ctctctccag cgaccagcgc caactcacag
     1561 cctgctaccc cgggaccctc cagctccaga cttatctatc acgtattatg aactctctcc
     1621 cgatgatcac tcctgctagg agggcgtcct tcatggagga aaagaagccc tgaagctgaa
     1681 ggaaagaaaa gctgggcaga atacgtgctt ttcggttgta aatactgtct tgccacttta
     1741 tgagaaaata gatttaactg aaagtcacat ttgcaataat gggttctcct ctgcctgttc
     1801 tttttgcttt cggttttttt tttttttttt tttttagctt ccgattgctt tagatacatg
     1861 attccagaaa tatttttctg ttggaggcaa ttaattgaca gttacttaga gtaattctta
     1921 acttatacat atatattgta aatattgcac atcaaaataa ctttggtatt tagagctcta
     1981 tatttttctt caaaataaca ttttaacagc ttggaatcca ttacagggaa ttgaaaatat
     2041 atttaacttt tgcttttctc tttaatcttt tgttaatagt gtatcatcaa atgaaaatat
     2101 aacagttgtg cctaatggta tatactttct taaaatcttt taatcatata atcttacatc
     2161 ttttcttata agaaatactt ctttcaatgt aagctataaa taatacattg agggcaattt
     2221 caaactatta aaaatgtaaa tttccccata aataacattg aaataactaa tttgtttctt
     2281 ggcctttaaa aataacatcc ccaatgaaat tagcaaacca tgaacacgaa acatttaaga
     2341 atgggttaaa tatgatcaca cagttagcct tgtagatatg tattgaaata atttatgaat
     2401 ttcttttaga tttgttgatg tcacttgtaa aaatatcaca tttccattgt aagcacattt
     2461 caagaatgcc tggtaaatga agcccccttt tctttgttgt tatttcatac aatgtccagt
     2521 tgtatataaa aaaaaggatt gtaaaatttt ataggataat atcatttgtt taagcaaaat
     2581 agcttaaaaa gtattatgtc attttactat atacagtact ttgccaatca tgagccaggt
     2641 tttattaact atttgtatat gccttaaaat aacttgataa ataaatgtac tattattatc
     2701 aataaaatat ttaaaggaga aaaaaaaaaa aaaaaaaaaa acccgaattc gagctcgccc
     2761 ggggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2821 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2881 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2941 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     3001 gatcctctag agtcgagccc aagcttgcat gcctgcaggt cgaccagctt ctgatggaat
     3061 tagaacttgg caaaacaata ctgagaatga agtgtatgtg gaacagaggc tgctgatctc
     3121 gttcttcagg ctatgaaact gacacatttg gaaaccacag tacttagaac cacaaagtgg
     3181 gaatcaagag aaaaacaatg atcccacgag agatctatag atctatagat catgagtggg
     3241 aggaatgagc tggcccttaa tttggttttg cttgtttaaa ttatgatatc caactatgaa
     3301 acattatcat aaagcaatag taaagagcct tcagtaaaga gcaggcattt atctaatccc
     3361 accccacccc cacccccgta gctccaatcc ttccattcaa aatgtaggta ctctgttctc
     3421 acccttctta acaaagtatg acaggaaaaa cttccatttt agtggacatc tttattgttt
     3481 aatagatcat caatttctgc atcccgggga tctgatatca tcgatgcatg gggtcgtgcg
     3541 ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc
     3601 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     3661 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     3721 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     3781 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     3841 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     3901 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     3961 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     4021 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     4081 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     4141 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg taagcttcag ctgctcgaga
     4201 tctagatgga tgcaggtcga aaggcccgga gatgaggaag aggagaacag cgcggcagac
     4261 gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc gcccgccccg
     4321 cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag
     4381 aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc
     4441 tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc
     4501 ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt
     4561 ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt
     4621 gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat
     4681 gctccagact gccttgggaa aagcgcctcc cctacccggt agaatttcga ggtcgaccag
     4741 cttctgatgg aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga
     4801 ggctgctgat ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag
     4861 aaccacaaag tgggaatcaa gagaaaaaca atgatcccac gagagatcta tagatctata
     4921 gatcatgagt gggaggaatg agctggccct taatttggtt ttgcttgttt aaattatgat
     4981 atccaactat gaaacattat cataaagcaa tagtaaagag ccttcagtaa agagcaggca
     5041 tttatctaat cccaccccac ccccaccccc gtagctccaa tccttccatt caaaatgtag
     5101 gtactctgtt ctcacccttc ttaacaaagt atgacaggaa aaacttccat tttagtggac
     5161 atctttattg tttaatagat catcaatttc tgcatcccgg ggatctgata tcatcgatgc
     5221 atggggtcgt gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc
     5281 gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac
     5341 ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc
     5401 gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc
     5461 cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg
     5521 ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa
     5581 ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt
     5641 ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc
     5701 gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc
     5761 gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct
     5821 ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tggtaagctt
     5881 cagctgctcg agatctagat ggatgcaggt cgaaaggccc ggagatgagg aagaggagaa
     5941 cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg
     6001 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
     6061 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
     6121 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc
     6181 catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg
     6241 aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt
     6301 ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc
     6361 tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagaattt
     6421 cgaggtcgac tctagaggat ccccgggtac cgagctcgaa ttaattcgcc ctatagtgag
     6481 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6541 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6601 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     6661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6721 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6781 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6841 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6901 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6961 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7021 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7081 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     7141 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7261 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7321 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8701 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9361 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9421 ctggaaagcg ggcagtgagc gcaacgcaat
//
