LOCUS       sequence-252801-        7198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::BAR:NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH114
FEATURES             Location/Qualifiers
     source          1..7198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2047..2598
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MGPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     misc_feature    2901..2925
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(3081..4229)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4565..5153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4645..4664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5324..6115,6116..6184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5947..5966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6185..6289)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6376..7007
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    7099..7123
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caatgccgaa ttcggatccg gagtgacccg gtcgtgcccc tctctagaga
       61 taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg
      121 tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat
      181 aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac
      241 atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt
      301 gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt
      361 tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta
      421 catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt
      481 tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat
      541 accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc
      601 agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc
      661 gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga
      721 gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag
      781 cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta
      841 cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag
      901 acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa
      961 ccagatcgat ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt
     1021 cctccccccc cccccctctc taccttctct agatcggcgt tccggtccat gattagggcc
     1081 cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg
     1141 ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca
     1201 gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc
     1261 atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata
     1321 tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga
     1381 tgtggtctgg ttgggcggtc gttctagatc ggagtagaat taattctgtt tcaaactacc
     1441 tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat
     1501 tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac
     1561 tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc
     1621 ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat
     1681 taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg
     1741 gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg
     1801 atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa
     1861 caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc
     1921 tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct
     1981 tttgtcgatg ctcaccctgt tgtttggtgt tacttgtgca ggtcgcccgg gcgaattcgg
     2041 acgtccatgg gcccagaacg acgcccggcc gacatccgcc gtgccaccga ggcggacatg
     2101 ccggcggtct gcaccatcgt caaccactac atcgagacaa gcacggtcaa cttccgtacc
     2161 gagccgcagg aaccgcagga gtggacggac gacctcgtcc gtctgcggga gcgctatccc
     2221 tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg cccctggaag
     2281 gcacgcaacg cctacgactg gacggccgag tcgaccgtgt acgtctcccc ccgccaccag
     2341 cggacgggac tgggctccac gctctacacc cacctgctga agtccctgga ggcacagggc
     2401 ttcaagagcg tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg catgcacgag
     2461 gcgctcggat atgccccccg cggcatgctg cgggcggccg gcttcaagca cgggaactgg
     2521 catgacgtgg gtttctggca gctggacttc agcctgccgg taccgccccg tccggtcctg
     2581 cccgtcaccg agatatgaga tatcactagt gttcaaacat ttggcaataa agtttcttaa
     2641 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     2701 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     2761 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     2821 ataaattatc gcgcgcggtg tcatctatgt tactgcgctg caattgtctt ctgcacgaag
     2881 tggtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     2941 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     3001 gtatgtgcat gccaaccaca gggttcccca gatcaggcgc tggctgctga acccccagcc
     3061 ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg
     3121 tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac
     3181 ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg
     3241 tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg
     3301 cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc
     3361 tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg
     3421 tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc
     3481 gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag
     3541 cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc
     3601 ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg
     3661 ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc
     3721 tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc
     3781 atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg
     3841 atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc
     3901 aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc
     3961 atcgacttcg ccaaacctgc cgcctcctgt tcaagacgac gcgaacgctc cacggcggcc
     4021 gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta
     4081 gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg
     4141 cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg
     4201 tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac
     4261 gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa
     4321 tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg
     4381 gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg
     4441 gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg
     4501 ccggcatcgt tgcgccacat ctaggatctg ccaggaaccg taaaaaggcc gcgttgctgg
     4561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5461 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6181 tcatactctt cctttttcaa tattattgaa gcatttatca aggttattgt ctcatgagcg
     6241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acgaattggc
     6301 cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga
     6361 tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg
     6421 tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt
     6481 taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa
     6541 tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt
     6601 cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc
     6661 tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac
     6721 tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc
     6781 gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt
     6841 caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc
     6901 ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat
     6961 agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgca aaggagatcc
     7021 tgatctgact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     7081 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     7141 acttaataac acattgcgga cgtttttaat gtactggggt ggatgcagtg ggccccac
//
