LOCUS       sequence-252803-       10838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH23
FEATURES             Location/Qualifiers
     source          1..10838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2029..2049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2074..6177
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2074..6177
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6178..6225
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6241..6493
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6541..6565
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6721..7869)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8205..8793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8285..8304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8964..9755,9756..9824))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9587..9606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9825..9929)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10016..10647
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10739..10763
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggcacc taaaaaaaag
     2041 aggaaggtgg ggatacatgg ggtgccagct gcaatggata aaaagtactc tatcgggctc
     2101 gatatcggca ctaactccgt ggggtgggcc gtcatcacag acgaatacaa ggtcccatcg
     2161 aaaaaattta aagttttggg caataccgac agacactcta tcaaaaagaa cctgattgga
     2221 gcgcttttgt ttgattctgg agaaacggcc gaagccaccc ggctcaagcg gaccgcccgt
     2281 cggcgctata ctcgtcggaa gaaccgcatt tgttaccttc aggagatctt ttcaaatgag
     2341 atggctaagg tggacgattc tttctttcac cggctggagg agtccttcct tgtcgaagag
     2401 gacaaaaaac acgagaggca tccaatattc ggcaacattg tggatgaagt ggcatatcac
     2461 gagaagtacc ccaccatata ccatcttaga aagaaacttg tcgactccac ggacaaggcc
     2521 gaccttcgtc tgatttacct cgcgctcgcc catatgatca agtttcgggg ccatttcctc
     2581 atcgagggag acttgaatcc tgacaattct gacgtcgaca aattgtttat ccaactcgtg
     2641 caaacgtaca accaactctt tgaggagaac ccaataaacg cctccggagt tgatgcaaaa
     2701 gctatcctga gcgcgcgcct ttcgaagtcc cggaggctgg agaacttgat cgcccaactc
     2761 ccaggcgaaa agaagaacgg cttgtttggc aatcttattg ccctgagcct cgggcttaca
     2821 cccaacttta agtcaaactt cgacctggca gaggatgcta agctccagct ctcgaaggac
     2881 acctacgacg acgatttgga caacctcttg gcccaaatcg gcgatcagta tgccgatttg
     2941 ttcctcgccg ccaaaaacct ctcggatgcg attctgctct ctgacatctt gcgcgtgaac
     3001 accgagataa cgaaggcgcc gctgtctgcg tccatgatca agcggtacga cgagcaccat
     3061 caggacctga ccctccttaa ggcgttggtg cgccaacaat tgcccgagaa gtataaggaa
     3121 atcttcttcg atcaatccaa gaacggctac gccggataca tagacggagg ggcctcccag
     3181 gaggagttct ataagtttat caagcctatt cttgaaaaga tggatggcac cgaggagctc
     3241 ctcgttaaac ttaacaggga ggaccttctc cgtaagcaaa gaaccttcga taatgggtca
     3301 ataccccacc aaatccacct cggagagctc cacgcgatcc tcagacggca ggaggatttt
     3361 tatccattcc tcaaagataa ccgtgagaaa atcgaaaaga tcctgacctt ccgcatccca
     3421 tactacgtcg ggcctttggc gcggggtaac agcagattcg cctggatgac caggaaatca
     3481 gaagagacaa taaccccgtg gaacttcgaa gaggttgttg acaagggtgc gtccgcacag
     3541 tctttcatcg aaaggatgac taactttgac aaaaaccttc ccaatgagaa agtgctccct
     3601 aagcattccc tgttgtatga gtacttcacc gtctacaacg agttgaccaa agtcaagtac
     3661 gtcacagagg ggatgcgcaa gccagccttt ctcagcggcg agcaaaagaa ggctattgtt
     3721 gacctcttgt tcaagacgaa caggaaggtc accgtgaagc agctgaagga ggattacttt
     3781 aagaagatag agtgcttcga ctcggtggag atttcagggg tcgaggaccg cttcaatgcc
     3841 tcacttggga cataccatga cctgcttaag attatcaagg acaaggactt tctggacaat
     3901 gaagagaacg aggacatctt ggaggacatt gtcctcacac tcacgctctt cgaggaccgg
     3961 gaaatgattg aagagagact caagacttat gcgcacttgt ttgatgacaa ggtgatgaag
     4021 caactcaaga ggcgcaggta cacaggctgg ggccgcctct cccgtaagct catcaatggc
     4081 atccgtgaca agcagtccgg aaagactata ttggactttc tgaagtccga cggtttcgcc
     4141 aaccggaatt tcatgcaact tatccacgat gactctctga cattcaagga agatatccaa
     4201 aaggcccagg tgagcggaca gggcgactct ctgcatgagc acatcgcgaa ccttgccgga
     4261 tccccggcca tcaagaaggg aatccttcag accgtgaagg ttgtcgatga gcttgttaag
     4321 gtgatgggca ggcacaagcc tgagaacatt gtcatagaga tggcccgtga gaatcagaca
     4381 acgcagaagg gccagaagaa ctccagagag cgtatgaaaa gaattgaaga agggattaag
     4441 gagttgggct cacaaatcct gaaggagcac ccagtcgaga atacgcagct gcagaacgag
     4501 aaactgtatc tgtattacct gcagaacgga cgcgacatgt atgtcgacca ggaactcgat
     4561 atcaatagac tcagcgatta tgacgtcgac catatcgtgc ctcaatcgtt cctcaaggat
     4621 gactccatcg ataacaaggt gctcacgaga tcggacaaga atcgcggcaa atccgacaac
     4681 gtcccgagcg aggaggttgt caagaaaatg aagaattact ggaggcaact gcttaacgca
     4741 aagctgatca cccagagaaa gttcgacaat ctcacgaagg cagagcgggg tggactttct
     4801 gaactggata aggcagggtt tattaaacgc cagctggtgg agacacgtca gatcacaaag
     4861 catgtggcgc agattctgga cagccggatg aatacgaagt atgacgagaa cgataagctg
     4921 atcagggagg tgaaagttat cacattgaag tccaagctcg tgtccgattt tcggaaggac
     4981 ttccagtttt ataaggttag ggagattaac aactatcacc acgctcacga cgcctatctg
     5041 aatgcggttg ttggcacggc actcattaaa aagtacccca agctggagtc cgagttcgtg
     5101 tacggtgact acaaggtgta cgacgtgcgc aagatgattg ctaagtctga acaagagata
     5161 ggcaaggcaa ccgccaagta ctttttctac tccaacataa tgaacttttt caaaaccgag
     5221 ataactcttg cgaatggcga gatccgcaaa cggccactta tcgagacgaa tggtgagact
     5281 ggagagatcg tgtgggacaa gggacgggat tttgcgactg ttaggaaggt gctttccatg
     5341 ccgcaagtga acatagtgaa gaaaacagag gttcaaacgg gaggctttag caaggaatcc
     5401 atcctcccca agaggaattc cgataagctc atagctagaa aaaaggactg ggacccaaag
     5461 aagtatggcg ggtttgactc accgaccgtc gcgtactcgg ttctcgtcgt tgcgaaggtt
     5521 gaaaagggta agtctaaaaa gctcaaatca gtgaaggagc tcctgggcat taccatcatg
     5581 gagaggtcat catttgagaa gaatccaatc gatttccttg aggccaaggg gtacaaggag
     5641 gtgaagaagg acctgataat caagctgcct aagtattcac tcttcgaact cgaaaacggg
     5701 aggaagagga tgctggcatc cgctggagag cttcagaagg gaaatgaact cgcccttcca
     5761 tccaagtacg tcaacttcct ttacctcgcc tcgcattacg agaagctcaa gggttcccct
     5821 gaagataatg agcaaaaaca gctcttcgtg gagcaacata agcattacct ggacgagatt
     5881 atcgaacaga tctccgagtt ttccaagcgt gtcatcctcg ctgacgctaa tctcgacaag
     5941 gttctctccg cctacaacaa acaccgggac aagccaatcc gggagcaagc cgaaaatatt
     6001 atccacctct tcacccttac taatctcggg gcacctgcgg cgtttaaata ctttgacacc
     6061 acgatagacc gcaagcgcta tacgtccacc aaggaggttc ttgacgcgac cctcattcac
     6121 cagagcatca caggcctcta cgaaacgagg atcgacctca gccagctggg tggggataaa
     6181 agacccgcgg caactaagaa agccggccaa gcgaaaaaga agaaatgagc ttgtcaagca
     6241 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     6301 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     6361 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     6421 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     6481 atgttactag atcgacgcta ctattgtctt ctgcacgaag tggtttaaac tatcagtgtt
     6541 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     6601 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     6661 gggttcccca gatcaggcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc
     6721 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
     6781 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
     6841 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
     6901 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
     6961 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
     7021 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
     7081 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
     7141 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
     7201 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
     7261 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
     7321 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
     7381 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
     7441 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
     7501 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt
     7561 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
     7621 cgcctcctgt tcaagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
     7681 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc
     7741 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
     7801 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
     7861 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta
     7921 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa
     7981 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg
     8041 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
     8101 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
     8161 ctaggatctg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8221 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8281 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8341 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8401 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8461 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8521 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8581 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8641 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8701 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8761 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8821 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8881 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8941 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9001 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9061 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg
     9121 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9181 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9241 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9301 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9361 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9421 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9481 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9541 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9601 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9661 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9721 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9781 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9841 tattattgaa gcatttatca aggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9901 tagaaaaata aacaaatagg ggttccgcgc acgaattggc cagcgctgcc atttttgggg
     9961 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    10021 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    10081 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    10141 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    10201 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    10261 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    10321 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    10381 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    10441 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    10501 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca
    10561 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg
    10621 gcgagggcaa ccagcccggt gagcgtcgca aaggagatcc tgatctgact gatgggctgc
    10681 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    10741 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    10801 cgtttttaat gtactggggt ggatgcagtg ggccccac
//
