LOCUS       sequence-252808-        2953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector for modules assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pDV
FEATURES             Location/Qualifiers
     source          1..2953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        44..91
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(436..452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(436..452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    560..681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(597..616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     651..670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(683..705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    694..710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(718..724,725..742,743..748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    763..784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(901..918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1072..1660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1152..1171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1831..2490)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2405..2424
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2491..2595)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2663..2681
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2719..2741)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2841..2860
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2930..2947
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2931..2947
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agatctctag aggatccgaa ttcgaagaca aatccagaga cctgttgaca attaatcatc
       61 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tgatagatcc
      121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      241 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc
      301 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc
      361 aaactggcag atgcacggtt acgattaagg tctcaccatt tgtcttctcg aggcggccgc
      421 acgcgtgtaa tcatggtcat agctgtttcc tgtgatcata atcagccata ccacatttgt
      481 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      541 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      601 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      661 caaactcatc aatgtatctt aacctgtgtg aaattgttat ccgctcacaa ttccacacaa
      721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1501 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1801 aagtatatat gagtaaactt ggtctgacag ttacgccccg ccctgccact catcgcagta
     1861 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     1921 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1981 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     2041 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     2101 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     2161 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     2221 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     2281 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2341 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     2401 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     2461 ggtggtatat ccagtgattt ttttctccat actcttcctt tttcaatatt attgaagcat
     2521 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2581 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2641 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     2701 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     2761 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     2821 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     2881 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgtt gtaaaacgac
     2941 ggccagtgtc gac
//
