LOCUS       sequence-252811-        3210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 2 module- Act1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pMV17
FEATURES             Location/Qualifiers
     source          1..3210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    138..159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(174..179,180..197,198..204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    212..228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     217..239
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     236..252
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     236..252
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(846..863)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(846..862)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(855..877)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1071..1090)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1190..1212
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1250..1268)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1336..1440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1441..1509,1510..2301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1659..1678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2472..3060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2961..2980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
       61 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      121 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      181 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      241 aacagctatg accatgatta cgccaagctt gcatgcaggc ctctggcagt cgacgggccc
      301 ggtctctgtt ctaatgttaa ttcagtaaat tttcgatctt gggaagaaaa aagcagtaag
      361 cgtgaaaaat ctaaaagctg atgtagtaga agatcctatt ctttaacaaa gattgacctt
      421 ttctttttct tcttggtttg agtagaaagg ggaaggaaga atacaagaga gaggagaaaa
      481 aggaagataa aaagagagcg tgatataaat gaatatatat tgaacaagag agattgggaa
      541 ggaaaggatc aaacaaaccc aaaaatattt caaaaaggag agagagaggc gagtttggtt
      601 tcaaaacggt ttatttattt atgcaagagg acgtggaaga aaaagaagaa ggaagaaaaa
      661 aatttgaaag aaaaaaacgc gtggcgggta aagaagaaaa tggaaaatag aggccgggtg
      721 acagagaaat attgagggtt aattagaaag tatgttaggg tgaggcatat gtttttaagg
      781 gttttgagca tacgagaccg ggatccgata tctagatgca ttcgcgaggt accgagctcg
      841 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      901 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      961 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     1021 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     1081 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     1141 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     1201 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     1261 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1321 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1381 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1441 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1501 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1561 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1621 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1681 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1741 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1801 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1861 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1921 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1981 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2041 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2101 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggatct
     2161 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2221 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2281 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2341 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2401 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2461 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2521 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2581 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2641 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2701 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2761 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2821 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2881 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2941 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3001 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3061 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3121 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3181 gataccgctc gccgcagccg aacgaccgag
//
