LOCUS       sequence-252812-        3437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 2 module- Adh1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pMV19
FEATURES             Location/Qualifiers
     source          1..3437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..76
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     68..85
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     69..85
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        143..847
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(906..922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(906..922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(919..941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    930..946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(954..960,961..978,979..984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    999..1020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1137..1154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1308..1896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1388..1407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2067..2858,2859..2927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2690..2709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2928..3032)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3100..3118
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3156..3178)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3278..3297
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
       61 acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa tgcatctaga
      121 tatcggatcc cggtctcgta tgatcctttt gttgtttccg ggtgtacaat atggacttcc
      181 tcttttctgg caaccaaacc catacatcgg gattcctata ataccttcgt tgatctccct
      241 aacatgtagg tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg
      301 gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata
      361 ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt
      421 gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct
      481 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag
      541 gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg
      601 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag
      661 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc
      721 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct
      781 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca
      841 atcaactgaa cagagaccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa
      901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      961 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1021 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1081 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1141 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1201 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1261 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1321 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1921 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1981 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2041 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2101 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca
     2221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3061 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3121 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3181 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3241 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3301 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3361 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     3421 tcgctattac gccagct
//
