LOCUS       sequence-252821-        3458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 4 module- sfGFP-STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pMV44
FEATURES             Location/Qualifiers
     source          1..3458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..36
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     230..252
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     244..261
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     245..261
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1103..1119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1103..1119)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1116..1138)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1127..1143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1151..1157,1158..1175,1176..1181))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1196..1217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1334..1351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1505..2093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1585..1604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2264..3055,3056..3124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2887..2906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3125..3229)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3297..3315
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3353..3375)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
       61 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
      121 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      181 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      241 cgttgtaaaa cgacggccag tgaattcgag ctcggtacct cgcgaatgca tctagatatc
      301 ggatcccggt ctcgcgcaat gtccaagggt gaagagctat ttactggggt tgtacccatt
      361 ttggtagaac tggacggaga tgtaaacgga cataaattct ctgttagagg tgagggcgaa
      421 ggcgatgcca ccaatggtaa attgactctg aagtttatat gcactacggg taaattacct
      481 gttccttggc caaccctagt aacaactttg acatatggtg ttcaatgttt ctcaagatac
      541 ccagaccata tgaaaaggca tgatttcttt aaaagtgcta tgccagaagg ctacgtgcaa
      601 gagagaacta tctcctttaa ggatgacggt acgtataaaa cacgagcaga agtgaaattc
      661 gaaggggata cactagttaa tcgcatcgaa ttaaagggta tagactttaa ggaagatggt
      721 aatattctcg gccataaact tgagtataat ttcaactcgc ataatgtgta cattacagct
      781 gacaaacaaa agaacggaat taaagcgaat tttaaaatca ggcacaacgt cgaagatggg
      841 tctgttcaac ttgccgatca ttatcagcaa aacaccccta ttggtgatgg tccagtcttg
      901 ttacccgata atcactactt aagcacacag tctagattgt caaaagatcc gaatgaaaag
      961 cgtgatcaca tggttttatt ggaatttgtc accgctgcag gaataactca cggaatggac
     1021 gagctttata agggaggggg ttgacaggag agaccgggcc cgtcgactgc agaggcctgc
     1081 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1141 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1201 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1261 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1321 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1381 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1441 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1501 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1561 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1621 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1681 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1741 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1801 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1921 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1981 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2041 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2101 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2161 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2221 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2281 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2341 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2401 cgcgagatcc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2461 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2521 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2581 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2641 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2701 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2761 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2821 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2881 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2941 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3001 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3061 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3121 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3241 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3301 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     3361 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     3421 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgg
//
