LOCUS       sequence-252823-        2961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 5 module- Adh1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pMV6
FEATURES             Location/Qualifiers
     source          1..2961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     119..136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    253..274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(289..294,295..312,313..319)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    327..343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     332..354
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     351..367
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     351..367
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(455..642)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     563..587
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(712..729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(712..728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(721..743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(937..956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1056..1078
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1116..1134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1202..1306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1307..1375,1376..2167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1525..1544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2338..2926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2827..2846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
       61 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      121 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      181 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      241 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      301 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      361 ctatgaccat gattacgcca agcttgcatg caggcctctg cagtcggcgg gcccggtctc
      421 ttgctatatt accctgttat ccctagcgga tctgccggta gaggtgtggt caataagagc
      481 gacctcatac tatacctgag aaagcaacct gacctacagg aaagagttac tcaagaataa
      541 gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta ttttttttat
      601 aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag aagtcctgcg
      661 agaccgggat ccgatatcta gatgcattcg cgaggtaccg agctcgaatt cactggccgt
      721 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      781 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      841 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
      901 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
      961 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     1021 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     1081 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     1141 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1201 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1261 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1321 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1381 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1441 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1501 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1561 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1621 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1681 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1741 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1801 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1861 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1921 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1981 tggctggttt attgctgata aatctggagc cggtgagcgt ggatctcgcg gtatcattgc
     2041 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2101 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2161 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2221 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2281 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2341 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2401 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2461 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2521 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2581 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2641 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2701 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2761 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2821 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2881 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2941 ggccttttta cggttcctgg c
//
