LOCUS       sequence-252852-       11285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  frameshift reporter for barcode retrieval.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Feldman D, Tsai F, Garrity AJ, O'Rourke R, Brenan L, Ho P, Gonzalez 
            E, Konermann S, Johannessen CM, Beroukhim R, Bandopadhayay P, 
            Blainey PC
  TITLE     CloneSifter: enrichment of rare clones from heterogeneous cell 
            populations.
  JOURNAL   BMC Biol. 2020 Nov 24;18(1):177. doi: 10.1186/s12915-020-00911-3.
  PUBMED    33234154
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2020-11-24"; volume: "18(1):177. doi"; pages: " 
            10.1186/s12915-020-00911-3"
COMMENT     Sequence Label: pLenti_TMv2
FEATURES             Location/Qualifiers
     source          1..11285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3864
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3855
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3812..3832
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3944..3997
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4247..4256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4720..4776
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4777..5169
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5185..5238
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5977..6565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6030..6050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6448..6459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6568..6584)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6569..6585)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7090..7270
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7296..7313)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7302..7526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7572..8000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7659..7678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7869..7890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8009..8029)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8014..8343
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8194..8329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8256..8275
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8391..8438
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8457..8831
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8961..9082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8998..9017)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9052..9071
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9131..9147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9131..9147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9144..9166)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9155..9171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9179..9185,9186..9203,9204..9209))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9224..9245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9362..9379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9533..10121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9613..9632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10292..11083,11084..11152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10915..10934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11153..11257)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccactggctg
     3001 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3061 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3121 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3181 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3241 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3301 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3361 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3421 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3481 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3541 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3601 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3661 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3721 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3781 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3841 tttcttccat ttcaggtgtc gtgaaccggt taatagtgag tagtagtaag tgataataga
     3901 aatcgagacg ctcagactca gacgtctctg tacaggcagt ggagagggca gaggaagtct
     3961 gctcacctgc ggcgacgtcg aggagaatcc tggcccagtc agcaagggcg aggaggacaa
     4021 cgcctctctc ccagcgacac acgagttaca catctttggc tccatcaacg gtgtggactt
     4081 cgacctcgtg ggtcagggca ccggcaatcc aaacgacggt tacgaggagc tcaacctcaa
     4141 gtccaccaag ggcgacctcc agttctcccc ctggattctg gtccctcata tcgggtacgg
     4201 cttccatcag tacctgccct acccagacgg gatgtcgcct ttccaggccg ccatggtgga
     4261 cggctccgga taccaagtcc atcgcacaat gcagttcgaa gacggtgcct cccttactgt
     4321 caactaccgc tacacctacg agggaagcca catcaaagga gaggcccagg tcaaggggac
     4381 tggtttccct gccgacggtc ctgtcctcac caactcgctc accgctgcgg actggtgcag
     4441 gtcgaagaag acttacccca acgacaaaac catcatcagt accttcaagt ggagttacac
     4501 cactggaaac ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc
     4561 aatggcggcc aactatctca agaaccagcc gatgtacgtg ttccgcaaga cggagctcaa
     4621 gcactccaag accgagctca acttcaagga gtggcaaaag gcctttaccg acgtcatggg
     4681 catggacgag ctctacaagt aataataacc ggatccggcg caacaaactt ctctctgctc
     4741 aaacaagccg gcgacgtcga agagaatcct gggccggcca agcctttgtc tcaagaagaa
     4801 tccaccctca tcgaaagagc aacggctaca atcaacagca tccccatctc cgaagactac
     4861 agcgtcgcca gcgcagctct ctcaagcgac ggccgcatct tcactggtgt caacgtatat
     4921 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     4981 gctggcaacc tcacttgtat cgtcgcgatc ggaaacgaga acaggggcat cctcagcccc
     5041 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catcgtcaag
     5101 gacagcgacg gacagccgac ggcagttggg attcgcgaat tgctgccctc tggttacgtg
     5161 tgggagggcc gtacgggcag tggggagggc agaggaagtc tgctaacctg cggtgacgtc
     5221 gaggagaatc ctggcccagt gtccaagggc gaggaagata acatggccat catcaaggag
     5281 ttcatgaggt ttaaggtcca catggagggt tcagtcaatg gccacgagtt cgagattgaa
     5341 ggcgagggcg agggccgccc ctacgaaggg acacagacgg cgaaattgaa ggtgaccaaa
     5401 ggcgggccat tgcccttcgc atgggacatc ttgtcccctc agtttatgta tggcagcaag
     5461 gcctatgtta agcaccccgc tgatatcccg gactacttga agctgtcctt tccagagggg
     5521 tttaaatggg agcgcgttat gaatttcgaa gacggaggag tggttacggt gacgcaggac
     5581 tcatccctgc aggacggaga atttatatat aaggttaagt tgagaggcac aaacttccca
     5641 agcgacggcc ctgtgatgca gaagaaaaca atggggtggg aagcttccag cgagcgcatg
     5701 taccccgaag atggcgccct caagggcgag ataaagcaaa ggctgaaact taaggacggc
     5761 ggtcattacg acgcggaggt caagacaact tacaaggcta aaaaacccgt tcagttgcct
     5821 ggggcttaca atgttaatat caaacttgac atcacaagcc acaatgaaga ctatacgatc
     5881 gtggagcagt atgaacgagc ggaaggcagg cactcaacgg gggggatgga cgagctttac
     5941 aagtaataat aagaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt
     6001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6361 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6421 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6481 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6541 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     6601 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     6661 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     6721 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6781 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     6841 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     6901 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     6961 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     7021 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     7081 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7141 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7201 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7261 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     7321 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7381 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7441 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     7501 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     7561 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7621 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7681 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7741 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7801 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7861 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7921 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7981 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8041 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8161 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8221 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8281 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8341 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     8401 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     8461 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     8521 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     8581 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     8641 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     8701 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     8761 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     8821 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8881 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8941 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     9001 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9061 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     9121 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9181 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9241 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9961 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11161 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11221 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11281 ctgac
//
