LOCUS       sequence-252853-       12615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homologous recombination vector for excision reporter 1 in the CLYBL
            locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12615)
  TITLE     Excision Reporter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP
FEATURES             Location/Qualifiers
     source          1..12615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(7..40)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     enhancer        111..414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..693
                     /label=chicken beta-actin promoter
     intron          694..1710
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1634..1656
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1718..1737
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1786..1819
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    1843..1876
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2043..2177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2297..2431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2460..2493
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2568..2577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2574..2576,2577..2579,2580..3290)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2619..2640)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2880..2899)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3227..3248
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3291..3347
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3348..3368
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3372..3392
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3396..3416
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3432..4163
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     primer_bind     complement(4250..4269)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4315..4370
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4369..4388)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    4703..4736
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(6792..6809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6792..6808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6801..6823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7017..7036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7136..7158
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7289..7308)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7300..8109
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7367..7386)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8287..8875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8776..8795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9029..9046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9163..9184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9199..9204,9205..9222,9223..9229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9237..9253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9242..9264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9261..9277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9261..9277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    10144..10191
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    10192..10217
                     /label=SA
                     /note="splice acceptor site"
     CDS             10241..10294
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10289..11089
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10349..10368)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10959..10978
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11124..11348
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    11349..11396
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
ORIGIN
        1 gtcgacataa cttcgtatag gatactttat acgaagttat caattcaggc tagttattaa
       61 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      121 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      181 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      241 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      301 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      361 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
      421 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
      481 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc
      541 gcgccrgggg gggsggggsg gggsgrgggg sggggsgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc
      721 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct
      901 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
      961 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1021 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc
     1081 tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg
     1141 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc
     1201 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
     1261 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
     1321 ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     1381 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     1441 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     1501 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     1561 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
     1621 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1681 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     1741 tctcatcatt ttggcaaaga attgttttga taccgcgggc cctaagaagt tcctattctc
     1801 tagaaagtat aggaacttcg tcgacattta aatcatttaa atataacttc gtataatgta
     1861 tgctatacga agttattcgc gatgaataaa tgaaagcttg cagatctgcg actctagagg
     1921 atctgcgact ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1981 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2041 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2101 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2161 cttatcatgt ctggatctgc gactctagag gatcataatc agccatacca catttgtaga
     2221 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2281 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2341 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2401 actcatcaat gtatcttatc atgtctggat ccccatcaag ctgatccgga acccttaata
     2461 taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgacctgc agcccaagct
     2521 agatcgaatt cggccggcct tgtacgcgtt aagtgcaaca cgatcccgcc accatggtga
     2581 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2641 taaacggcca caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc
     2701 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2761 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2821 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2881 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2941 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3001 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3061 aggcgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3121 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3181 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3241 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gctactaact
     3301 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctcca aaaaagaaga
     3361 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtacacg
     3421 tgtcaacgcg tatggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc
     3481 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
     3541 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     3601 tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca
     3661 cccagggcat ccccgatttc tttaagcagt ccttccctga gggcttcaca tgggagagag
     3721 tcaccacata cgaggacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     3781 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
     3841 tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg
     3901 gcctggaagg cagaaccgac atggccctga agctcgtggg cgggggccac ctgatctgca
     3961 acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
     4021 actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
     4081 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
     4141 atggcatgga cgagctgtac aagtaagaat tcactcctca ggtgcaggct gcctatcaga
     4201 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     4261 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     4321 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     4381 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     4441 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     4501 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     4561 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     4621 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     4681 ttctcttatg gagatcaccg gtataacttc gtataatgta tactatacga agttatggta
     4741 ccttaattaa gttaacatgc atagtactga gctcacgggg acagcccccc cccaaagccc
     4801 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     4861 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     4921 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     4981 cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca
     5041 gaatcataga acggcctggg ttgcaaagga gcacagtgct catccagatc caaccccctg
     5101 ctatgtgcag ggtcatcaac cagcagccca ggctgcccag agccacatcc agcctggcct
     5161 tgaatgcctg cagggatggg gcatccacag cctccttggg caacctgttc agtgcgtcac
     5221 caccctctgg gggaaaaact gcctcctcat atccaaccca aacctcccct gtctcagtgt
     5281 aaagccattc ccccttgtcc tatcaagggg gagtttgctg tgacattgtt ggtctggggt
     5341 gacacatgtt tgccaattca gtgcatcacg gagaggcaga tcttggggat aaggaagtgc
     5401 aggacagcat ggacgtggga catgcaggtg ttgagggctc tgggacactc tccaagtcac
     5461 agcgttcaga acagccttaa ggataagaag ataggataga aggacaaaga gcaagttaaa
     5521 acccagcatg gagaggagca caaaaaggcc acagacactg ctggtccctg tgtctgagcc
     5581 tgcatgtttg atggtgtctg gatgcaagca gaaggggtgg aagagcttgc ctggagagat
     5641 acagctgggt cagtaggact gggacaggca gctggagaat tgccatgtag atgttcatac
     5701 aatcgtcaaa tcatgaaggc tggaaaagcc ctccaagatc cccaagacca accccaaccc
     5761 acccaccgtg cccactggcc atgtccctca gtgccacatc cccacagttc ttcatcacct
     5821 ccagggacgg tgaccccccc acctccgtgg gcagctgtgc cactgcagca ccgctctttg
     5881 gagaaggtaa atcttgctaa atccagcccg accctcccct ggcacaacgt aaggccatta
     5941 tctctcatcc aactccagga cggagtcagt gagaatattg tttaaactcc tcatccttcc
     6001 aacataaata tattttggga ttatatcaac attcaatgtt acttaaagtg accttgtaaa
     6061 tattttcaca actgagccat gtttgatttg tatacttatg tttactttac tgtttttcct
     6121 gaagttaata attgccttga atttatttat ttctttaaaa atgtttcatt actcaggact
     6181 gtagtttaca ttacgattct ttgtgttata cagttgatgg gtttcttttc tttcttaatt
     6241 tctttaaaaa atagagatgg ggtcttacta tattacccag gctggtcttg aagtcctggg
     6301 ctcaagtgat cttcctgtct cagcctacca agtagctgag actataggtg caaaaaagcc
     6361 actatacctg gctagtttac aggttttaac aaatgcatta tgccacgtat ccattattac
     6421 aggatcacac aagatatttt cattaccctg aaagcatccc tgtgttccac caattcatcc
     6481 tgcctccatg agccgctggc aaccactgat ctctatagtt ttgccttttc taaaatgtca
     6541 tataattgga atcatacagt ctgtagcatt ttcagactag cttttaaaat ttggcaatat
     6601 gcatttaagg ttcctcctta aatgtgaagg gcatagccaa tgtggcttga tagctcattt
     6661 ctttttattg gtgaatattt cattgtctgg atgttccaca gtttgtttat ccattcacct
     6721 attcaatttg ctttttttct gtgtatctat cactaattca atactggact ctccaacaga
     6781 gccgtttaaa cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6841 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6901 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     6961 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     7021 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     7081 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7141 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgatgca gctctggccc
     7201 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     7261 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     7321 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     7381 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     7441 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     7501 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     7561 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     7621 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     7681 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     7741 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     7801 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     7861 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     7921 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     7981 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     8041 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     8101 tttttctaat cagaattggt taattggttg taacattatt cagattgggc ttgatttaaa
     8161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9061 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9181 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9241 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgccg cggccgcagc
     9301 aggagaatca tgccaatggg ccaatataca ttctgaccca cagtttcata ataaaataaa
     9361 atggttgtgg ttgtaagcca ctatgtttca gagtggtttg ttacacagca ataaataact
     9421 aatatagtag gcataccatc aagtccaaag taggtagaga agaatgtaaa tagcagagca
     9481 aaacagcatg actggtggct gggaggctta aaactgggac aggatcagag tcatgaaaga
     9541 agtcaaagaa atggttcaga agtaaggctg agactgactt acaaaagctg aaagtccctt
     9601 taagttggtg tttggtgcat tggcaggggc aggtatggtg acttaaaaga gccatgctca
     9661 acaagatcaa gcacaacaca atcacgggtc accccagcag accttagcga gtctagccat
     9721 ttctttggtg gtggtcacag tcatgcttca gcccagtttc cacttggaca aatggtacat
     9781 attttcaatg agatgaaaat taagatacaa tccatgtgct cagagagtga tcacagctct
     9841 gactaaacac tgtgccccaa agtgttgagg aattgggaaa acctagctga gttagtggtc
     9901 tcttttctgt tacaataaag ctcataatga aaattagcct tctttgttct tccccaagtc
     9961 tttctttcta gacgaaacta ctttcaactg ttttaacttc cttactgtta acttccatat
    10021 ttctagatag tatggtcaga ctgttatttc ttgactttta aatgtaagat attatctact
    10081 gacttccttc tatgtaagat gaggattgag ctctcttacc cttctcccag cggccgcatc
    10141 gatgaagttc ctattccgaa gttcctattc tctgaaaagt ataggaactt cctgacctct
    10201 tctcttcctc ccacagggcc tcgagagatc tggcagcgga gagggcagag gaagtcttct
    10261 aacatgcggt gacgtggagg agaatcccgg ccctaggatt gaacaagatg gattgcacgc
    10321 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
    10381 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
    10441 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
    10501 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
    10561 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
    10621 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
    10681 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
    10741 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
    10801 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
    10861 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
    10921 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
    10981 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
    11041 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctaac acctggtgca
    11101 tgacccgcaa gcccggtgcc tgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    11161 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    11221 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    11281 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    11341 ctctatggga agttcctatt ccgaagttcc tattctctga aaagtatagg aacttcattt
    11401 aaatgagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
    11461 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
    11521 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
    11581 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
    11641 aaaagcttta ggctgaaaga gagatttaga atgacagaat catagaacgg cctgggttgc
    11701 aaaggagcac agtgctcatc cagatccaac cccctgctat gtgcagggtc atcaaccagc
    11761 agcccaggct gcccagagcc acatccagcc tggccttgaa tgcctgcagg gatggggcat
    11821 ccacagcctc cttgggcaac ctgttcagtg cgtcaccacc ctctggggga aaaactgcct
    11881 cctcatatcc aacccaaacc tcccctgtct cagtgtaaag ccattccccc ttgtcctatc
    11941 aagggggagt ttgctgtgac attgttggtc tggggtgaca catgtttgcc aattcagtgc
    12001 atcacggaga ggcagatctt ggggataagg aagtgcagga cagcatggac gtgggacatg
    12061 caggtgttga gggctctggg acactctcca agtcacagcg ttcagaacag ccttaaggat
    12121 aagaagatag gatagaagga caaagagcaa gttaaaaccc agcatggaga ggagcacaaa
    12181 aaggccacag acactgctgg tccctgtgtc tgagcctgca tgtttgatgg tgtctggatg
    12241 caagcagaag gggtggaaga gcttgcctgg agagatacag ctgggtcagt aggactggga
    12301 caggcagctg gagaattgcc atgtagatgt tcatacaatc gtcaaatcat gaaggctgga
    12361 aaagccctcc aagatcccca agaccaaccc caacccaccc accgtgccca ctggccatgt
    12421 ccctcagtgc cacatcccca cagttcttca tcacctccag ggacggtgac ccccccacct
    12481 ccgtgggcag ctgtgccact gcagcaccgc tctttggaga aggtaaatct tgctaaatcc
    12541 agcccgaccc tcccctggca caacgtaagg ccattatctc tcatccaact ccaggacgga
    12601 gtcagtgaga atatt
//
