LOCUS       sequence-252884-        9729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Gata3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Benchetrit H, Herman S, van Wietmarschen N, Wu T, Makedonski K, Maoz
            N, Yom Tov N, Stave D, Lasry R, Zayat V, Xiao A, Lansdorp PM, Sebban
            S, Buganim Y
  TITLE     Extensive Nuclear Reprogramming Underlies Lineage Conversion into 
            Functional Trophoblast Stem-like Cells.
  JOURNAL   Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 
            10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
  PUBMED    26412562
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.08.006"; journalName: "Cell Stem Cell"; date: 
            "2015-11-5- 5"; volume: "17"; issue: "5"; pages: "543-56"
COMMENT     Sequence Label: FUW-TetO-Gata3
FEATURES             Location/Qualifiers
     source          1..9729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        136..515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        517..715
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             733..913
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    960..1085
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1578..1811
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1996..2040
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2338..2455
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2512..2530,2531..2553,2554..2572,2573..2595,2596..2614,
                     2615..2637,2638..2656,2657..2679,2680..2698,2699..2721,
                     2722..2740,2741..2763,2764..2782)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2815..2853
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2850..2874
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4319..4907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4372..4392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4790..4801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4910..4926)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4911..4927)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5432..5612
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5638..5655)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5644..5868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5914..6342
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6001..6020)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6211..6232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6351..6371)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6356..6685
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6536..6671
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6598..6617
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6733..6780
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6799..7173
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7303..7424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7340..7359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7394..7413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7473..7489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7473..7489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7486..7508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7497..7513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7521..7527,7528..7545,7546..7551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7566..7587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7704..7721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7875..8463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7955..7974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8634..9425,9426..9494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9257..9276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9495..9599)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9674..9693)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
       61 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
      121 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
      721 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      781 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      841 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      901 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      961 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1021 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1081 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1141 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1201 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1261 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1321 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1381 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1441 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1501 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1561 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1621 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1681 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1741 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1801 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1861 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1921 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1981 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2041 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2101 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2161 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2221 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2281 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     2341 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     2401 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     2461 ttattacagg gacagcagag atccagtttg gttagatctc gagtttacca ctccctatca
     2521 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2581 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2641 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2701 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2761 cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg
     2821 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     2881 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
     2941 ggccccgaat tcggcttatg gaggtgactg cggaccagcc gcgctgggtg agccaccatc
     3001 accccgcggt cctcaacggt cagcacccag acacgcacca cccgggcctc ggccattcgt
     3061 acatggaagc tcagtatccg ctgacggaag aggtggacgt actttttaac atcgatggtc
     3121 aaggcaacca cgtcccgtcc tactacggaa actccgtcag ggctacggtg cagaggtatc
     3181 ctccgaccca ccacgggagc caggtatgcc gcccgcctct gctgcacgga tctctgccct
     3241 ggctggatgg cggcaaagcc ctgagcagcc accacaccgc ctcgccctgg aacctcagcc
     3301 ccttctccaa gacgtccatc caccacggct ctccggggcc tctgtccgtt taccctccgg
     3361 cttcatcctc ttctctggcg gccggccact ccagtcctca tctcttcacc ttcccgccca
     3421 ccccgccgaa agacgtctcc ccagacccgt cgctgtccac cccgggatcc gccgggtcgg
     3481 ccaggcaaga tgagaaagag tgcctcaagt atcaggtgca gctgccagat agcatgaagc
     3541 tggagacgtc tcactctcga ggcagcatga ccaccctggg tggggcctca tcctcagccc
     3601 accaccccat taccacctat ccgccctatg tgcccgagta cagctctgga ctcttcccac
     3661 ccagcagcct gctgggagga tcccctaccg ggttcggatg taagtcgagg cccaaggcac
     3721 gatccagcac agaaggcagg gagtgtgtga actgcggggc aacctctacc ccactgtggc
     3781 ggcgagatgg taccgggcac tacctttgca atgcctgcgg actctaccat aaaatgaatg
     3841 ggcagaaccg gccccttatc aagcccaagc gaaggctgtc ggcagcaagg agagcaggga
     3901 catcctgcgc gaactgtcag accaccacca ccaccctctg gaggaggaac gctaatgggg
     3961 acccggtctg caatgcctgt gggctgtact acaagcttca taatattaac agacccctga
     4021 ctatgaagaa agaaggcatc cagacccgaa accggaagat gtctagcaaa tcgaaaaagt
     4081 gcaaaaaggt gcatgacgcg ctggaggact tccccaagag cagctccttc aacccggccg
     4141 ctctctccag acacatgtca tccctgagcc acatctctcc cttcagccac tccagccaca
     4201 tgctgaccac accgacgccc atgcatccgc cctccggcct ctccttcgga cctcaccacc
     4261 cttccagcat ggtcaccgcc atgggttaga agccgaattc gatatcaagc ttatcgataa
     4321 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4381 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4441 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4501 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4561 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4621 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4681 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4741 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4801 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4861 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     4921 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     4981 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5041 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5101 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5161 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     5221 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     5281 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     5341 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     5401 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     5461 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5521 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5581 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     5641 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5701 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5761 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5821 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5881 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5941 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6001 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6061 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6121 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6181 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6241 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6301 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     6361 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6421 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6481 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6541 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6601 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6661 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6721 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     6781 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     6841 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     6901 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     6961 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     7021 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     7081 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     7141 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     7201 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7261 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     7321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     7441 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7501 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7561 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7621 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7681 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7741 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7801 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7861 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7921 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7981 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8041 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8101 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8161 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8221 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8281 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8341 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8401 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8461 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8521 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8581 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8641 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8701 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8761 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8821 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9421 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9481 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9541 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9601 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     9661 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9721 cttgtgtgt
//
