LOCUS       sequence-252885-        9791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Tfap2c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Benchetrit H, Herman S, van Wietmarschen N, Wu T, Makedonski K, Maoz
            N, Yom Tov N, Stave D, Lasry R, Zayat V, Xiao A, Lansdorp PM, Sebban
            S, Buganim Y
  TITLE     Extensive Nuclear Reprogramming Underlies Lineage Conversion into 
            Functional Trophoblast Stem-like Cells.
  JOURNAL   Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 
            10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
  PUBMED    26412562
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.08.006"; journalName: "Cell Stem Cell"; date: 
            "2015-11-5- 5"; volume: "17"; issue: "5"; pages: "543-56"
COMMENT     Sequence Label: FUW-TetO-Tfap2c
FEATURES             Location/Qualifiers
     source          1..9791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    176..293
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(350..368,369..391,392..410,411..433,434..452,453..475,
                     476..494,495..517,518..536,537..559,560..578,579..601,
                     602..620)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        653..691
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..712
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2219..2807
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2272..2292)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2690..2701)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2810..2826)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2811..2827)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3332..3512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3538..3555)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3544..3768
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3814..4242
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3901..3920)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4111..4132
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4251..4271)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4256..4585
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4436..4571
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4498..4517
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4633..4680
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4699..5073
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5203..5324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5240..5259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5294..5313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5373..5389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5373..5389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5386..5408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5397..5413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5421..5427,5428..5445,5446..5451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5466..5487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5604..5621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5775..6363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5855..5874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6534..7325,7326..7394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7157..7176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7395..7499)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7574..7593)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7765..8144
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8146..8344
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8362..8542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8589..8714
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9207..9440
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9625..9669
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
       61 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
      121 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
      181 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      241 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      301 attacaggga cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt
      361 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      421 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
      481 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
      541 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      601 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
      661 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
      721 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
      781 ccccgaattc ggcttcgcca tgttgtggaa aataacagat aatgtcaagt atgaagagga
      841 ttgcgaggat cgccacgact cgagcagtaa tggcaaccct cgcatccctc acctctcctc
      901 tcccggacaa catctctaca gtcccgcgcc gcctctctcg cacaccgggg ttgcagagta
      961 ccagccgcct ccttacttcc cgccgcctta ccagcagctg gcatactcgc agtccgccga
     1021 ccattactcg catctgggag aggcttacgc tgccgccatg aaccccctgc accagcctgc
     1081 ggccaccggc agccagcaac aggcctggcc gggtcgacag agtcaggagg gctctagcct
     1141 ggcctcgcac cacagccgct ctgcaagtct aataccccat atttcagggc tggagggggg
     1201 ctcggtgagc gcccggcggg aagtctaccg ccggtccgac ctgctgctgc ctcacgcgca
     1261 cgccctggaa gccggcctgg ctgagaacct ggggctgcac gagatggctc accccataga
     1321 ggaggtgcag aatgtggacg acgcgcactt gctcctacac gatcagactg tcattcgcaa
     1381 aggacccatt tcgatgacca agaacccttt ggggctccct tgccagaagg acctggtggg
     1441 agtggtcatg aaccccagtg aggtcttctg ctcagtccct ggaagactgt ccctgctcag
     1501 ctccacgtcg aagtacaaag taactgtggc tgaggtacag aggcgactgt caccaccgga
     1561 atgcctaaac gcctcgctcc tgggaggtgt gctcagaaga gcaaagtcca aaaatggagg
     1621 ccggtccttg agggagaagt tggacaaaat tggattgaac cttccggccg ggagacggaa
     1681 agctgcccac gtcactctcc tcacgtctct cgtggaaggt gaagccgtcc acctagcacg
     1741 ggactttgcc tatgtctgcg aagctgagtt ccctagtaaa gcggtggctg actatttaac
     1801 gagaccacat cttgggggac ggaatgagat ggccacgcgg aagagtatgt tgttggctgc
     1861 acagcaggtg tgcaaggagt tcactgacct tctccatcaa gatcggacac ccaacgggaa
     1921 caacaggccc gcccaggtct tggagccgaa catacaaaac tgtttgtctc atttcagcct
     1981 gataactcat ggctttggca gccaggccat ctgtgcggcg gtctccgcag tgcagaatta
     2041 tatcaaggag gctctaatcg ccatcgataa gtcctacatg aacccggggg accagagtcc
     2101 ggctgattcc agcaagacga tggagaaaat ggaaaagcac aggaagtaaa atggctgcaa
     2161 ggaggccaga gagcagaagg agaggaccca agccgaattc gatatcaagc ttatcgataa
     2221 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2281 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2341 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2401 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2461 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2521 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2581 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     2641 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     2701 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2761 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     2821 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     2881 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     2941 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3001 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     3061 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     3121 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     3181 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     3241 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     3301 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     3361 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3421 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3481 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     3541 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3601 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3661 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3721 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3781 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3841 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3901 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3961 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4021 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4081 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4141 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4201 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4261 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4321 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4381 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4441 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4501 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4561 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     4621 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     4681 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     4741 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     4801 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     4861 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     4921 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     4981 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     5041 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     5101 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5161 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     5221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     5341 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5401 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5461 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5521 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5581 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5641 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5701 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5761 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5821 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5881 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5941 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6001 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6061 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6121 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6181 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6241 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6301 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6361 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6421 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6841 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6961 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7081 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7141 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7201 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7261 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7321 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7381 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7441 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7501 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     7561 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     7621 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     7681 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     7741 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     7801 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     7861 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7921 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7981 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     8041 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     8101 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     8161 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     8221 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     8281 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     8341 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8401 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8461 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8521 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     8581 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     8641 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     8701 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     8761 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     8821 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     8881 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     8941 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9001 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     9061 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9121 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9181 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9241 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9301 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9361 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9421 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9481 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     9541 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9601 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     9661 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9721 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     9781 agagttaggc a
//
