LOCUS       sequence-252901-       10436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RAF1 H466R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-RAF1-H466R
FEATURES             Location/Qualifiers
     source          1..10436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5396..5420)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5428..5446
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5471..5490)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5472..5490)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5496..5516)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5570..5611
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5632..6199
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5649..6201
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5793..5810)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6020..6039
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6213..6812
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6213..6232)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6709..6729
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6826..7414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6879..6899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7297..7308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7489..7722
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7841..7859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7927..8031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8032..8100,8101..8892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8250..8269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9063..9651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9552..9571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9805..9822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10062..10083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10098..10103,10104..10121,10122..10128)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10136..10152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10141..10163
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10160..10176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10160..10176
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10226..10245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagcacatac agggagcttg gaagacgatc
     3481 agcaatggtt ttggattcaa agatgccgtg tttgatggct ccagctgcat ctctcctaca
     3541 atagttcagc agtttggcta tcagcgccgg gcatcagatg atggcaaact cacagatcct
     3601 tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat
     3661 gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cactcaaggt gaggggcctg
     3721 caaccagagt gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc
     3781 ttagattgga atactgatgc tgcgtctttg attggagaag aacttcaagt agatttcctg
     3841 gatcatgttc ccctcacaac acacaacttt gctcggaaga cgttcctgaa gcttgccttc
     3901 tgtgacatct gtcagaaatt cctgctcaat ggatttcgat gtcagacttg tggctacaaa
     3961 tttcatgagc actgtagcac caaagtacct actatgtgtg tggactggag taacatcaga
     4021 caactcttat tgtttccaaa ttccactatt ggtgatagtg gagtcccagc actaccttct
     4081 ttgactatgc gtcgtatgcg agagtctgtt tccaggatgc ctgttagttc tcagcacaga
     4141 tattctacac ctcacgcctt cacctttaac acctccagtc cctcatctga aggttccctc
     4201 tcccagaggc agaggtcgac atccacacct aatgtccaca tggtcagcac caccctgcct
     4261 gtggacagca ggatgattga ggatgcaatt cgaagtcaca gcgaatcagc ctcaccttca
     4321 gccctgtcca gtagccccaa caatctgagc ccaacaggct ggtcacagcc gaaaaccccc
     4381 gtgccagcac aaagagagcg ggcaccagta tctgggaccc aggagaaaaa caaaattagg
     4441 cctcgtggac agagagattc aagctattat tgggaaatag aagccagtga agtgatgctg
     4501 tccactcgga ttgggtcagg ctcttttgga actgtttata agggtaaatg gcacggagat
     4561 gttgcagtaa agatcctaaa ggttgtcgac ccaaccccag agcaattcca ggccttcagg
     4621 aatgaggtgg ctgttctgcg caaaacacgg catgtgaaca ttctgctttt catggggtac
     4681 atgacaaagg acaacctggc aattgtgacc cagtggtgcg agggcagcag cctctacaaa
     4741 cacctgcatg tccaggagac caagtttcag atgttccagc taattgacat tgcccggcag
     4801 acggctcagg gaatggacta tttgcatgca aagaacatca tccgtagaga catgaaatcc
     4861 aacaatatat ttctccatga aggcttaaca gtgaaaattg gagattttgg tttggcaaca
     4921 gtaaagtcac gctggagtgg ttctcagcag gttgaacaac ctactggctc tgtcctctgg
     4981 atggccccag aggtgatccg aatgcaggat aacaacccat tcagtttcca gtcggatgtc
     5041 tactcctatg gcatcgtatt gtatgaactg atgacggggg agcttcctta ttctcacatc
     5101 aacaaccgag atcagatcat cttcatggtg ggccgaggat atgcctcccc agatcttagt
     5161 aagctatata agaactgccc caaagcaatg aagaggctgg tagctgactg tgtgaagaaa
     5221 gtaaaggaag agaggcctct ttttccccag atcctgtctt ccattgagct gctccaacac
     5281 tctctaccga agatcaaccg gagcgcttcc gagccatcct tgcatcgggc agcccacact
     5341 gaggatatca atgcttgcac gctgaccacg tccccgaggc tgcctgtctt ctagaaccca
     5401 gctttcttgt acaaagtggt gatatccaat taaccctcac taaaggatga tgtgtttgat
     5461 ttgaattgaa ccctatagtg agtcgtatta ccacccaact tttctataca aagtggttga
     5521 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     5581 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc
     5641 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     5701 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5761 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5821 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5881 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5941 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     6001 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     6061 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     6121 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     6181 ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc
     6241 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6301 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6361 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6421 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6481 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6541 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6601 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6661 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6721 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6781 cctggtgcat gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat
     6841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7201 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7381 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     7441 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7501 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7561 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7621 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7681 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7741 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     7801 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     7861 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8641 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9781 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9841 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     9901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
    10021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    10081 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    10141 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
    10201 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10261 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10321 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    10381 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
