LOCUS       sequence-252915-        9647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Sox17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, 
            Zakheim Z, Radwan A, Maoz N, Lasry R, Renous N, Inbar M, Ram O, 
            Kaplan T, Buganim Y
  TITLE     Direct Induction of the Three Pre-implantation Blastocyst Cell Types
            from Fibroblasts.
  JOURNAL   Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 
            10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
  PUBMED    31031139
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.03.018"; journalName: "Cell Stem Cell"; date: 
            "2019-06-6- 6"; volume: "24"; issue: "6"; pages: "983-994.e7"
COMMENT     Sequence Label: FUW-TetO-Sox17
FEATURES             Location/Qualifiers
     source          1..9647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    149..266
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(323..341,342..364,365..383,384..406,407..425,426..448,
                     449..467,468..490,491..509,510..532,533..551,552..574,
                     575..593)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        626..664
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     661..685
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2048..2636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2101..2121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2519..2530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2639..2655)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2640..2656)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3161..3341
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3367..3384)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3373..3597
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3643..4071
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3730..3749)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3940..3961
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4080..4100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4085..4414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4265..4400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4327..4346
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4462..4509
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4528..4902
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5032..5153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5069..5088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5123..5142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5202..5218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5202..5218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5215..5237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5226..5242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5250..5256,5257..5274,5275..5280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5295..5316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5433..5450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5604..6192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5684..5703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6363..7154,7155..7223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6986..7005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7224..7328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7403..7422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7594..7973
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7975..8173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8191..8371
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8418..8543
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9036..9269
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9454..9498
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
       61 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
      121 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
      181 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      241 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
      301 ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      361 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      421 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      481 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      541 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      601 aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag
      661 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      721 tagaagacac cgggaccgat ccagcctccg cggccccgaa ttgatgagca gcccggatgc
      781 gggatacgcc agtgacgacc agagccagcc ccggagcgcg cagcccgcgg tgatggcagg
      841 gttgggcccc tgtccctggg ccgagtccct gagccccctc ggggatgtaa aggtgaaagg
      901 cgaggtggtg gcgagtagcg gggcgccagc cgggacgtcg ggccgagcca aagcggagtc
      961 tcgcatccgg cggccgatga acgcctttat ggtgtgggcc aaagacgaac gcaagcggtt
     1021 ggcacagcag aacccagatc tgcacaacgc agagctaagc aagatgctag gcaagtcttg
     1081 gaaggcgttg accttggcag agaagcggcc cttcgtggaa gaggccgagc ggctgcgcgt
     1141 gcagcatatg caggaccacc ccaactacaa gtaccggccg cggcggcgca agcaggtgaa
     1201 gcgcatgaag cgggtggagg gaggcttcct gcacgctctc gtcgagcccc aggccggcgc
     1261 gcttggtccc gagggcggcc gcgtggccat ggatggcctg ggtctgcctt tcccggagcc
     1321 gggctatccg gccggtcctc cgctgatgtc tccgcacatg ggcccccact atcgggactg
     1381 ccagggactg ggcgctcccg cgctcgacgg ctaccctctg cccactccgg acacatcccc
     1441 gctggatggc gtggagcagg acccggcttt ctttgcagcc ccgctgccag gggactgccc
     1501 ggcggccggc acctacactt acgctccagt ctcggactat gcagtgtccg tagagccgcc
     1561 cgctggcccc atgcgagtgg ggccggaccc ctcgggccct gcgatgccgg ggatcctggc
     1621 gccccccagc gctctgcacc tgtactacgg cgcgatgggc tcgcccgccg caagtgcggg
     1681 gcgcggtttc cacgcgcaac cccagcagcc gctgcaaccg caggcaccgc cgccgccacc
     1741 gcagcagcag cacccagcgc acggccccgg gcaaccttcg ccccctcccg aggctctgcc
     1801 ctgccgggat ggcacggaat ccaaccagcc cactgagctc ctaggggagg tggaccgcac
     1861 ggaattcgaa cagtatctgc cctttgtgta taagcccgag atgggtcttc cctaccaggg
     1921 acacgactgc ggagtgaacc tctcagacag ccacggagcc atttcctccg tggtgtccga
     1981 cgctagctca gcggtctact attgcaacta ccccgacatt tgacaattcg atatcaagct
     2041 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2101 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2161 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2221 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2281 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2341 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2401 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2461 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2521 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2581 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2641 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     2701 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     2761 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2821 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     2881 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     2941 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     3001 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     3061 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     3121 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     3181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3301 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     3361 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3421 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3481 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3541 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3601 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3661 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3721 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3781 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3841 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3901 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3961 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4021 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4081 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4141 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4201 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4261 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4321 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4381 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4441 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     4501 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     4561 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     4621 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     4681 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     4741 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     4801 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     4861 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     4921 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4981 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     5041 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5101 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5161 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5281 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5341 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6001 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7321 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     7381 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     7441 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     7501 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     7561 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     7621 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7681 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7741 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7801 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7861 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7921 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7981 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     8041 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     8101 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     8161 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8221 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8281 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8341 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8401 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8461 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8521 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8581 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8641 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8701 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8761 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8821 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8881 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8941 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     9001 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     9061 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     9121 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     9181 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     9241 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     9301 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     9361 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     9421 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9481 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9541 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9601 atagtgaata gagttaggca gggatattca ccattatcgt ttcagac
//
