LOCUS       sequence-252920-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Cdx2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, 
            Zakheim Z, Radwan A, Maoz N, Lasry R, Renous N, Inbar M, Ram O, 
            Kaplan T, Buganim Y
  TITLE     Direct Induction of the Three Pre-implantation Blastocyst Cell Types
            from Fibroblasts.
  JOURNAL   Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 
            10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
  PUBMED    31031139
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.03.018"; journalName: "Cell Stem Cell"; date: 
            "2019-06-6- 6"; volume: "24"; issue: "6"; pages: "983-994.e7"
COMMENT     Sequence Label: FUW-TetO-Cdx2
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(86..104,105..127,128..146,147..169,170..188,189..211,
                     212..230,231..253,254..272,273..295,296..314,315..337,
                     338..356)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        389..427
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     424..448
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1497..2085
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1550..1570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1968..1979)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2088..2104)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2089..2105)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2610..2790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2816..2833)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2822..3046
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3092..3520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3179..3198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3389..3410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3529..3549)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3534..3863
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3714..3849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3776..3795
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3911..3958
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3977..4351
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4481..4602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4518..4537)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4572..4591
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4651..4667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4651..4667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4664..4686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4675..4691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4699..4705,4706..4723,4724..4729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4744..4765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4882..4899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5053..5641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5133..5152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5812..6603,6604..6672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6435..6454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6673..6777)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6852..6871)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7043..7422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7424..7622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7640..7820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7867..7992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8485..8718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8903..8947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9245..9333,1..29)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
       61 tttggttaga tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      121 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      181 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      241 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
      301 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
      361 tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
      421 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      481 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcggct tatgtacgtg
      541 agctaccttc tggacaagga cgtgagcatg tatcctagct ccgtgcgcca ctccggcggc
      601 ctgaacctgg ctccgcagaa ctttgtcagt cctccgcagt acccggacta cggtggttac
      661 cacgtggcgg ccgcggcggc tgctacggcg aacttggaca gcgctcagtc cccagggcca
      721 tcctggccca ccgcgtacgg cgcccctctc cgcgaggact ggaatggcta cgcacccggg
      781 ggcgctgcgg cagccaacgc ggtagcccac ggtctcaatg gtggctcccc ggccgccgct
      841 atgggctaca gcagccccgc cgaataccac gcgcaccatc acccgcatca tcacccgcac
      901 catccggccg cctcgccgtc ctgcgcctcc ggcttgctgc agacgctcaa cctcggcccc
      961 ccggggcccg cagccaccgc cgccgccgaa cagctgtccc ccagcggcca gcggcgaaac
     1021 ctgtgcgagt ggatgcggaa gcccgcgcag cagtccctag gaagccaagt gaaaaccagg
     1081 acaaaagaca aataccgggt ggtgtacaca gaccatcagc ggctggagct ggagaaggag
     1141 tttcacttta gtcgatacat caccatcagg aggaaaagtg agctggctgc cacacttggg
     1201 ctctccgaga ggcaggttaa aatttggttt cagaaccgca gagccaagga gaggaaaatc
     1261 aagaagaagc agcagcagca acagcagcag cagcaacaac agcctccaca gccgccgcca
     1321 caaccttccc agcctcagcc gggtgccctg cggagcgtgc cggagccctt gagtcctgtg
     1381 acctccttgc aaggctcagt gcctggttct gtccctgggg ttctggggcc agctggaggg
     1441 gttttaaact ccactgtcac ccagtgaaag ccgaattcga tatcaagctt atcgataatc
     1501 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1561 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1621 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     1681 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     1741 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     1801 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1861 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     1921 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     1981 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2041 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     2101 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     2161 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     2221 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     2281 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     2341 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     2401 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     2461 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     2521 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     2581 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     2641 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2701 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2761 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     2821 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2881 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2941 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3001 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3061 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3121 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3181 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3241 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3301 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3361 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3421 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3481 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     3541 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3601 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3661 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3781 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     3841 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     3901 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     3961 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     4021 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     4081 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     4141 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     4201 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     4261 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     4321 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     4381 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4441 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     4501 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4561 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     4621 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4681 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4741 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4801 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4861 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4921 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5461 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5701 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5761 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5821 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5881 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5941 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6001 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6061 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6121 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6181 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6241 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6301 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6361 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6421 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6481 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6541 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6601 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6661 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6721 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6781 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     6841 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     6901 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     6961 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     7021 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     7081 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     7141 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     7201 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     7261 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     7321 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     7381 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     7441 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     7501 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     7561 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     7621 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7681 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7741 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7801 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7861 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7921 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7981 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8041 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8101 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8161 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8221 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8281 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8341 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8401 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8461 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8521 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8581 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8641 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8701 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8761 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8821 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8881 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8941 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9001 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9061 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9121 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     9181 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     9241 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9301 taatagcaac agacatacaa actaaagaat tac
//
