LOCUS       sequence-252924-        9728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Klf5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, 
            Zakheim Z, Radwan A, Maoz N, Lasry R, Renous N, Inbar M, Ram O, 
            Kaplan T, Buganim Y
  TITLE     Direct Induction of the Three Pre-implantation Blastocyst Cell Types
            from Fibroblasts.
  JOURNAL   Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 
            10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
  PUBMED    31031139
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.03.018"; journalName: "Cell Stem Cell"; date: 
            "2019-06-6- 6"; volume: "24"; issue: "6"; pages: "983-994.e7"
COMMENT     Sequence Label: FUW-TetO-Klf5
FEATURES             Location/Qualifiers
     source          1..9728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        272..310
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     307..331
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1775..2363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1828..1848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2246..2257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2366..2382)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2367..2383)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2888..3068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3094..3111)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3100..3324
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3370..3798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3457..3476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3667..3688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3807..3827)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3812..4141
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3992..4127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4054..4073
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4189..4236
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4255..4629
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4759..4880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4796..4815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4850..4869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4929..4945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4929..4945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4942..4964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4953..4969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4977..4983,4984..5001,5002..5007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5022..5043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5160..5177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5331..5919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5411..5430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6090..6881,6882..6950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6713..6732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6951..7055)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7130..7149)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7321..7700
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7702..7900
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7918..8098
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8145..8270
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8763..8996
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9181..9225
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9523..9640
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9697..9715,9716..9728,1..10,11..29,30..52,53..71,
                     72..94,95..113,114..136,137..155,156..178,179..197,198..220,
                     221..239)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
       61 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      121 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      181 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
      241 aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat
      301 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      361 cctccataga agacaccggg accgatccag cctccgcggc cccgaattca tgcccacgcg
      421 ggtgctgacc atgagcgccc gcctgggacc actgccccag ccgccggccg cgcaggacga
      481 gcccgtgttc gcgcagctca agccggtgct gggcgctgcg aacccggccc gcgacgcggc
      541 gctcttctcc ggagacgatc tgaaacacgc gcaccaccac ccgcctgcgc cgccgccagc
      601 cgctggcccg cgactgccct cggaggagct ggtccagaca agatgtgaaa tggagaagta
      661 tctgacccct cagctccctc cagttccgat aatttcagag cataaaaagt atagacgaga
      721 cagtgcctca gtggtagacc agttcttcac tgacactgaa ggcatacctt acagcatcaa
      781 catgaacgtc ttcctccctg acatcactca cctgagaact ggcctctaca aatcccagag
      841 accatgcgta acacagatca agacagaacc tgttaccatt ttcagccacc agagcgagtc
      901 gacggcccct cctcctcctc cggcccccac ccaggctctc cccgagttca ctagtatctt
      961 cagctcccac cagaccacag cgccaccaca ggaggtgaac aatatcttca tcaaacaaga
     1021 acttcctata ccagatcttc atctctctgt cccttcccag cagggccacc tgtaccagct
     1081 gttgaataca ccggatctag acatgcccag ttcgacaaac cagacggcag taatggacac
     1141 tcttaatgtt tctatggcag gccttaaccc acacccctct gctgttccac agacgtcaat
     1201 gaaacagttc cagggcatgc ccccttgcac gtacaccatg ccaagtcagt ttcttccaca
     1261 gcaggccacc tactttcccc cgtcaccacc aagctcagag cctggaagtc ccgatagaca
     1321 agctgagatg ctgcagaatc tcaccccacc tccgtcctat gccgctacaa ttgcttccaa
     1381 actggcgatt cacaacccaa atttacctgc cactctgcca gttaattcgc caactctccc
     1441 acctgtcaga tacaacagaa ggagtaaccc ggatctggag aagcgacgta tccacttctg
     1501 cgattataat ggttgcacaa aagtttatac aaagtcgtct cacttaaaag ctcacctgag
     1561 gactcatacg ggcgagaagc cctacaagtg cacctgggag ggctgcgact ggaggtttgc
     1621 ccggtcggat gagctgaccc gccactacag gaagcacacg ggcgccaagc cgttccagtg
     1681 catggtgtgc caacgcagct tctcccgctc cgaccacctc gcgctgcaca tgaagcgcca
     1741 ccagaactga gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     1801 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1861 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1921 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1981 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2041 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2101 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2161 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2221 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2281 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2341 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     2401 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     2461 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     2521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     2581 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     2641 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     2701 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     2761 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     2821 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     2881 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3061 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3121 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3181 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3241 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3301 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3361 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3421 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3661 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3721 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3781 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3841 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3901 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3961 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4021 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4081 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4141 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     4201 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     4261 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     4321 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     4381 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     4441 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     4501 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     4561 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     4621 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4681 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4741 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4801 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4861 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     4921 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4981 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5101 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5761 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6061 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6121 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6181 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6241 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6301 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6361 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6421 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6481 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6541 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6601 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6661 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6721 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6781 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6841 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6901 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6961 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7021 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7081 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7141 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7201 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7261 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     7321 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7381 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7441 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7501 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7561 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7621 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7681 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7741 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7801 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7861 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     7921 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7981 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8041 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8101 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8161 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8221 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8281 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8341 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8401 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8461 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8521 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8581 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8641 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8701 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8761 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8821 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8881 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8941 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9001 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9061 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     9121 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     9181 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     9241 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9301 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9361 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     9421 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     9481 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     9541 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     9601 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     9661 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     9721 gaaagtcg
//
