LOCUS       sequence-252930-       10459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RAF1 S257L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-RAF1-S257L
FEATURES             Location/Qualifiers
     source          1..10459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5396..5420)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5473..5514
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5535..6102
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5552..6104
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5696..5713)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5923..5942
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6116..6118,6119..6121,6122..6835)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6116..6118,6119..6121,6122..6832)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6161..6182)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6422..6441)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6769..6790
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6849..7437
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6902..6922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7320..7331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7512..7745
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7864..7882)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7950..8054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8055..8123,8124..8915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8273..8292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9086..9674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9575..9594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9828..9845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10085..10106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10121..10126,10127..10144,10145..10151)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10159..10175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10164..10186
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10183..10199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10183..10199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10249..10268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagcacatac agggagcttg gaagacgatc
     3481 agcaatggtt ttggattcaa agatgccgtg tttgatggct ccagctgcat ctctcctaca
     3541 atagttcagc agtttggcta tcagcgccgg gcatcagatg atggcaaact cacagatcct
     3601 tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat
     3661 gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cactcaaggt gaggggcctg
     3721 caaccagagt gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc
     3781 ttagattgga atactgatgc tgcgtctttg attggagaag aacttcaagt agatttcctg
     3841 gatcatgttc ccctcacaac acacaacttt gctcggaaga cgttcctgaa gcttgccttc
     3901 tgtgacatct gtcagaaatt cctgctcaat ggatttcgat gtcagacttg tggctacaaa
     3961 tttcatgagc actgtagcac caaagtacct actatgtgtg tggactggag taacatcaga
     4021 caactcttat tgtttccaaa ttccactatt ggtgatagtg gagtcccagc actaccttct
     4081 ttgactatgc gtcgtatgcg agagtctgtt tccaggatgc ctgttagttc tcagcacaga
     4141 tattctacac ctcacgcctt cacctttaac acctccagtc cctcatctga aggttccctc
     4201 tcccagaggc agaggttgac atccacacct aatgtccaca tggtcagcac caccctgcct
     4261 gtggacagca ggatgattga ggatgcaatt cgaagtcaca gcgaatcagc ctcaccttca
     4321 gccctgtcca gtagccccaa caatctgagc ccaacaggct ggtcacagcc gaaaaccccc
     4381 gtgccagcac aaagagagcg ggcaccagta tctgggaccc aggagaaaaa caaaattagg
     4441 cctcgtggac agagagattc aagctattat tgggaaatag aagccagtga agtgatgctg
     4501 tccactcgga ttgggtcagg ctcttttgga actgtttata agggtaaatg gcacggagat
     4561 gttgcagtaa agatcctaaa ggttgtcgac ccaaccccag agcaattcca ggccttcagg
     4621 aatgaggtgg ctgttctgcg caaaacacgg catgtgaaca ttctgctttt catggggtac
     4681 atgacaaagg acaacctggc aattgtgacc cagtggtgcg agggcagcag cctctacaaa
     4741 cacctgcatg tccaggagac caagtttcag atgttccagc taattgacat tgcccggcag
     4801 acggctcagg gaatggacta tttgcatgca aagaacatca tccatagaga catgaaatcc
     4861 aacaatatat ttctccatga aggcttaaca gtgaaaattg gagattttgg tttggcaaca
     4921 gtaaagtcac gctggagtgg ttctcagcag gttgaacaac ctactggctc tgtcctctgg
     4981 atggccccag aggtgatccg aatgcaggat aacaacccat tcagtttcca gtcggatgtc
     5041 tactcctatg gcatcgtatt gtatgaactg atgacggggg agcttcctta ttctcacatc
     5101 aacaaccgag atcagatcat cttcatggtg ggccgaggat atgcctcccc agatcttagt
     5161 aagctatata agaactgccc caaagcaatg aagaggctgg tagctgactg tgtgaagaaa
     5221 gtaaaggaag agaggcctct ttttccccag atcctgtctt ccattgagct gctccaacac
     5281 tctctaccga agatcaaccg gagcgcttcc gagccatcct tgcatcgggc agcccacact
     5341 gaggatatca atgcttgcac gctgaccacg tccccgaggc tgcctgtctt ctagaaccca
     5401 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     5461 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     5521 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5581 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5641 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5701 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5761 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5821 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5881 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5941 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     6001 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     6061 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt
     6121 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     6181 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     6241 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     6301 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     6361 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     6421 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     6481 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     6541 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     6601 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     6661 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6721 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6781 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga
     6841 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6901 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6961 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7021 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7081 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7141 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7201 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7261 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7321 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7381 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7441 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7501 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     7561 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7621 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7681 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7741 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7801 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     7861 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8281 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9841 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9901 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9961 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    10021 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10081 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10141 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    10201 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10261 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10321 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10381 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10441 gtactacaaa cttagtagt
//
