LOCUS       sequence-252933-       11981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH93
FEATURES             Location/Qualifiers
     source          1..11981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(782..1282,1283..1378,1379..1876,1877..1972)
                     /codon_start=1
                     /product="adenine base editor comprising wild-type E. coli 
                     TadA fused to the evolved TadA*(7.10) mutant (Gaudelli et 
                     al., 2017)"
                     /label=ABE(7.10)
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARD
                     EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT
                     FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECA
                     ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             6107..6127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6143..6395
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6443..6467
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6623..7771)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8107..8695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8187..8206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8866..9657,9658..9726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9489..9508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9727..9831)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9918..10549
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10641..10665
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttccgcagac gggatcgatt tcatgatttt ttttgtttcg ttgcataggg tttggtttgc
       61 ccttttcctt tatttcaata tatgccgtgc acttgtttgt cgggtcatct tttcatgctt
      121 ttttttgtct tggttgtgat gatgtggtct ggttgggcgg tcgttctaga tcggagtaga
      181 attctgtttc aaactacctg gtggatttat taattttgga tctgtatgtg tgtgccatac
      241 atattcatag ttacgaattg aagatgatgg atggaaatat cgatctagga taggtataca
      301 tgttgatgcg ggttttactg atgcatatac agagatgctt tttgttcgct tggttgtgat
      361 gatgtggtgt ggttgggcgg tcgttcattc gttctagatc ggagtagaat actgtttcaa
      421 actacctggt gtatttatta attttggaac tgtatgtgtg tgtcatacat cttcatagtt
      481 acgagtttaa gatggatgga aatatcgatc taggataggt atacatgttg atgtgggttt
      541 tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta
      601 cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat
      661 gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat
      721 ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcagg
      781 aatgtccgaa gtcgagtttt cccatgagta ctggatgaga cacgcattga ctctcgcaaa
      841 gagggcttgg gatgaacgcg aggtgcccgt gggggcagta ctcgtgcata acaatcgcgt
      901 aatcggcgaa ggttggaata ggccgatcgg acgccacgac cccactgcac atgcggaaat
      961 catggccctt cgacagggag ggcttgtgat gcagaattat cgacttatcg atgcgacgct
     1021 gtacgtcacg cttgaacctt gcgtaatgtg cgcgggagct atgattcact cccgcattgg
     1081 acgagttgta ttcggtgccc gcgacgccaa gacgggtgcc gcaggttcac tgatggacgt
     1141 gctgcatcac ccaggcatga accaccgggt agaaatcaca gaaggcatat tggcggacga
     1201 atgtgcggcg ctgttgtccg acttttttcg catgcggagg caggagatca aggcccagaa
     1261 aaaagcacaa tcctctactg actctggtgg ttcttctggt ggttctagcg gcagcgagac
     1321 tcccgggacc tcagagtccg ccacacccga aagttctggt ggttcttctg gtggttcttc
     1381 cgaagtcgag ttttcccatg agtactggat gagacacgca ttgactctcg caaagagggc
     1441 tcgagatgaa cgcgaggtgc ccgtgggggc agtactcgtg ctcaacaatc gcgtaatcgg
     1501 cgaaggttgg aatagggcaa tcggactcca cgaccccact gcacatgcgg aaatcatggc
     1561 ccttcgacag ggagggcttg tgatgcagaa ttatcgactt atcgatgcga cgctgtacgt
     1621 cacgtttgaa ccttgcgtaa tgtgcgcggg agctatgatt cactcccgca ttggacgagt
     1681 tgtattcggt gttcgcaacg ccaagacggg tgccgcaggt tcactgatgg acgtgctgca
     1741 ttacccaggc atgaaccacc gggtagaaat cacagaaggc atattggcgg acgaatgtgc
     1801 ggcgctgttg tgttactttt ttcgcatgcc caggcaggtc tttaacgccc agaaaaaagc
     1861 acaatcctct actgactctg gtggttcttc tggtggttct agcggcagcg agactcccgg
     1921 gacctcagag tccgccacac ccgaaagttc tggtggttct tctggtggtt ctgagaaaaa
     1981 gtattctatt gggctggcca ttggcaccaa ttccgttgga tgggccgtga tcactgatga
     2041 ttataaggtc ccctccaaga agttcaaggt tctgggtaat accaacagga agagcataaa
     2101 aaagaatctt atgggcgctc tgctctttga tagcggagaa acggcggaag ctacacggct
     2161 caagcggaca gcgaggcgcc gctataccag gcggaagaac cggattagat accttcagga
     2221 aatcttcgcg aacgagatgg ccaagctcga tgactcattc ttccagcgcc ttgaggaatc
     2281 gttcctcgtt gaggaggaca aaaagaacga gcgtcacccc atctttggta atctcgcgga
     2341 cgaggttgca taccacagga attatccgac catctaccac ctgaggaaga agttggcgga
     2401 cagcccggag aaggcagacc tgcggctcat ctacctcgcg ctggcgcaca tcatcaaatt
     2461 ccgggggcat tttttgatcg aagggaaact caatgctgag aactcggacg tggcaaagct
     2521 cttctatcag cttatccaaa cctataatca gctgttcgag gaatctccgt tggatgagat
     2581 agaggttgac gctaagggta tcctctccgc tcgtctttcg aagtccaaac gcctggagaa
     2641 actgatcgcc gttttcccga acgagaagaa gaacgggctc ttcggaaaca taatcgcgct
     2701 tgccctggga ctgacgccca acttcaagtc caacttcgac ttgacggagg atgccaaact
     2761 tcagctttca aaggacacat atgacgatga tttggacgag ttgctcggcc aaattggcga
     2821 ccaatacgct gacctgttct ctgccgcaaa aaacctgtcc gatgctattc tgctgagcga
     2881 tattctccgg agcaactccg aggtgactaa ggcaccgttg tccgccagca tggtgaaacg
     2941 ctacgacgag caccaccagg acctggctct cctcaagacc ttggttaggc aacagtttcc
     3001 cgagaagtac gccgaaatct ttaaggatga caccaagaac ggctatgccg gttacgttgg
     3061 catcggaatc aagcatagga agaggaccac taaactcgcc acacaggagg aattctataa
     3121 atttatcaag cctattctgg agaagatgga cggagctgag gagctgctcg ccaagctgaa
     3181 cagggacgat ctcctgagga agcagcggac ctttgataat ggctcgattc ctcatcaaat
     3241 ccatctgaag gagcttcatg ccatactgcg caggcaagaa gagttttacc catttctgaa
     3301 ggagaatcgg gagaagatcg aaaaaatcct gacattccgc ataccctact acgtgggccc
     3361 gcttgcccgg ggaaactctc gttttgcttg gctcacgagg aagagcgagg aggcgataac
     3421 gccatggaac ttcgaggagg tcgtggataa aggcgccagc gcgcaatcgt ttattgagcg
     3481 tatgacaaac ttcgacgagc agctcccaaa caagaaggtg ttgccaaagc acagcctcct
     3541 gtacgagtac ttcacggttt acaatgagct caccaaagtc aagtacgtta ctgagcgtat
     3601 gcgtaagccg gagttcctct ctggggagca gaagaaggca atcgttgacc tgctcttcaa
     3661 aacgaaccgt aaggtgaccg tcaagcagct gaaggaagat tacttcaaaa agatcgaatg
     3721 ttttgactcc gtggagatta taggagttga ggataggttt aatgcctcac tcggcaccta
     3781 ccatgacttg ctcaagatca tcaaagacaa ggatttcctg gacaatgaag agaacgagga
     3841 tatccttgag gacatcgtgc tcacactcac cctcttcgag gaccgggaga tgatcgagga
     3901 gcgcctcaag acatacgccc acctctttga cgacaaggtg atgaaacaac tcaagcgcag
     3961 gcactacacg ggttggggac gcctctcccg caagatgatt aatggtatca gggacaaaca
     4021 gtccggtaag acgattctcg atttcttgaa gtccgatggt ttctctaatc gcaacttcat
     4081 gcagctgatc cacgacgact cactcacctt caaggaggag attgaaaagg ctcaagtgtc
     4141 cgggcaggga gatagcctcc acgagcaaat cgcagatctc gccggatcgc ctgcaattaa
     4201 gaagggcatt cttcaaactg tcaaaatcgt cgacgagctg gtcaaggtta tgggccacaa
     4261 gccagaaaac atcgttatcg agatggctag ggagaaccag accactacca agggactcca
     4321 acagtcccgg gagcggaaga agaggattga ggaggggatc aaggaactcg agtctcaaat
     4381 tctcaaggag aatccggtgg agaacactca actccagaac gagaagctct acctgtacta
     4441 cctgcagaac ggcagggata tgtatgtcga tcaggagctg gacatcaaca ggctctccga
     4501 ctacgacgtc gaccatatcg tcccgcaatc tttcattaag gacgactcaa tcgataacaa
     4561 ggtcctcacg cgctccgtcg agaacagagg taagtcagac aacgtgccgt cggaggaggt
     4621 ggtgaagaag atgaaaaact actggcgtca actcctcaac gctaaattga ttacgcagag
     4681 aaaattcgat aacttgacca aagcggaacg cggcggtctg tcggaggccg acaaagccgg
     4741 cttcattaag cggcaactcg tggagacacg ccagatcaca aagcacgtcg ccaggattct
     4801 tgactcgcgg atgaatacta aacgcgacaa gaacgataag ccaatcagag aagtcaaggt
     4861 gattacactg aagagcaaac tggtcagcga cttccgcaag gatttccagc tttataaggt
     4921 gagggacata aacaattatc accacgccca cgacgcgtac ctcaacgcgg tggttggcac
     4981 cgcactcatt aagaaatacc cgaagctcga gtcagagttc gtctacggcg actataaagt
     5041 gtacgacgtt cggaaaatga tagcaaagtc cgagcaagag atcggcaagg ccacggccaa
     5101 gagattcttc tactccaata ttatgaattt ctttaagacg gaggttaagt tggccaacgg
     5161 agagatccgg aagcggcctc tcattgaaac taatggcgag acaggcgagg tggtctggaa
     5221 caaggaaaag gactttgcta ccgtccgcaa ggtcctcgcg atgccgcaag tgaacattgt
     5281 caaaaagacg gaagttcaga caggcgggtt ctcgaaggag tcaatcttgt cgaagcgtga
     5341 gtccgccaag ctcatcccac gcaagaaggg gtgggatacg cggaagtatg gaggtttcgg
     5401 gtcccccact gttgcatact caattctcgt ggtcgccaag gtggagaagg gaaaagccaa
     5461 gaagctcaaa agcgtgaagg tcctcgtggg tataacgata atggaaaagg gtagctacga
     5521 gaaggaccca atcggtttcc tcgaagccaa ggggtacaaa gatatcaaaa aggagcttat
     5581 attcaaactg ccgaagtact cattgtttga gctggagaac ggcaggcggc ggatgttggc
     5641 ctcggcgaca gaactgcaaa aggcgaacga gctcgtcttg cctcagcatc tcgttaggct
     5701 tctctactac acccaaaata tctctgcgac tacagggtct aacaacctcg gttacatcga
     5761 gcagcatagg gaggaattca aggagatctt tgagaagatc atcgacttct ccgagaagta
     5821 catccttaag aacaaagtga actccaactt gaagtcgagc ttcgacgagc agttcgctgt
     5881 ttccgattct attctcctgt cgaactcatt cgtgtcactt ctgaagtaca cgtcgttcgg
     5941 cgcatctggc ggctttacct tcctcgacct cgacgtgaag caaggccggc tccgctacca
     6001 aaccgtgacc gaggttctcg acgcgacatt gatctaccag tccattaccg gcctgtacga
     6061 aacgaggacg gacctctcac agctcggagg ggactctaga gcggacccaa agaagaagcg
     6121 caaggtgtag gcttgtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt
     6181 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     6241 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     6301 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     6361 attatcgcgc gcggtgtcat ctatgttact agatcgacgc tactattgtc ttctgcacga
     6421 agtggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     6481 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     6541 ttgtatgtgc atgccaacca cagggttccc cagatcaggc gctggctgct gaacccccag
     6601 ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg
     6661 cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc
     6721 acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg
     6781 ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct
     6841 tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt
     6901 cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc
     6961 ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg
     7021 tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc
     7081 agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc
     7141 gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga
     7201 cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg
     7261 cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg
     7321 gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct
     7381 tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg
     7441 ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg
     7501 tcatcgactt cgccaaacct gccgcctcct gttcaagacg acgcgaacgc tccacggcgg
     7561 ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg
     7621 tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg
     7681 tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc
     7741 tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc
     7801 acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat
     7861 aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact
     7921 tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt
     7981 gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt
     8041 cgccggcatc gttgcgccac atctaggatc tgccaggaac cgtaaaaagg ccgcgttgct
     8101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9001 accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9721 actcatactc ttcctttttc aatattattg aagcatttat caaggttatt gtctcatgag
     9781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacgaattg
     9841 gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg
     9901 gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg
     9961 cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg
    10021 tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca
    10081 aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct
    10141 gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc
    10201 cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa
    10261 actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg
    10321 ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt
    10381 gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg
    10441 ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc
    10501 atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg caaaggagat
    10561 cctgatctga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
    10621 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
    10681 caacttaata acacattgcg gacgttttta atgtactggg gtggatgcag tgggccccac
    10741 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
    10801 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
    10861 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
    10921 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
    10981 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
    11041 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
    11101 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
    11161 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
    11221 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
    11281 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
    11341 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
    11401 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
    11461 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
    11521 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
    11581 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
    11641 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
    11701 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
    11761 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
    11821 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
    11881 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
    11941 ccagtgtttc tctttgggga atcctgggat ggctctagcc g
//
