LOCUS       sequence-252934-       10106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in of nuclear EYFP into mouse Elf5 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, 
            Zakheim Z, Radwan A, Maoz N, Lasry R, Renous N, Inbar M, Ram O, 
            Kaplan T, Buganim Y
  TITLE     Direct Induction of the Three Pre-implantation Blastocyst Cell Types
            from Fibroblasts.
  JOURNAL   Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 
            10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
  PUBMED    31031139
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.03.018"; journalName: "Cell Stem Cell"; date: 
            "2019-06-6- 6"; volume: "24"; issue: "6"; pages: "983-994.e7"
COMMENT     Sequence Label: pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS
FEATURES             Location/Qualifiers
     source          1..10106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..95
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2189..2242
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2258..2260,2261..2263,2264..2974)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2303..2324)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2564..2583)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2978..2998
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3002..3022
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3032..3065)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3083..3116
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    3159..3383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3372..3389
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3421..4224)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3532..3551)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4142..4161
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4238..4737)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4286..4305)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4679..4702
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    4758..4791
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(4808..4841)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6337..6346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6390..6494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6495..6563,6564..7355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6713..6732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7526..8114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8015..8034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8392..8454
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             8533..9663
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(9661..9680)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9705..9753
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgccgc cggaccaact
       61 aaacctgact acggaccggt aaaacgacgg ccagtgttaa cgtggagagg ctggtgtttg
      121 tttccataca tgtgaccaaa atcatgactt tcacttggac ttggaatttt acgaattaaa
      181 gaaatgttct atattctatt actgaaggag ggctttgttt cctgagccat tctgcggcct
      241 tctccttgaa gtacccaggg gaagaggata aagggatggg aatgtgggga gttgagcggt
      301 ccaggagcct ttcccagcgc tgagatctgc ccggggcaga atctagccac ttaaccattg
      361 gcagtagagc aggtacctta ttgcaccttt gacacctaca gcaactaaat agagtctggc
      421 attcccagag ccaggaaatt agggagtcat ggcccagatc attcttatcc atgccctaaa
      481 ggcacaagaa aatgccagct ccccgtgata agtgaactgc agcatagaca tcttcacaca
      541 accctcctgc tcatcagcaa ggactgagtt catgaggagc tgcagggaat tgggtaaatc
      601 tggtgtctct gcacggggat tttaccagta gttatcagaa aacagacttg tcccagcttg
      661 gtaaggctcc atgcagagtg ctccaagtga gagttgtcca gcccagcaac agttcttaca
      721 ggttaattag ttacctctct atatacatac atatagataa atatgtatat acatgtatat
      781 acatatacat ccttccctcc caccccactg tcttttaaca cctttcattt ctttaacacc
      841 tctcatttct ttttattctt ttaagattac aattacatca tttctccctt cctgctcctc
      901 cctccaaacc ttcccacata cacctcccca ttcttcttca aatccatggt ttcttttttc
      961 attaatagtc attgcattta catatgtgtg tatatatata tgtgtatata tacatacaca
     1021 cacatatata tatatacata catatatata ttcctaaata taacctgttc agtccatata
     1081 atgttactcg tatgtatatc ttcaggcctg accgcctggt actggaagcc agttggtgtc
     1141 ctcttccctg gggcacctct tccactccca gcactcctca gttgcctgta gttctttgtg
     1201 taacactgag gcatcgtggg ctttcccatg tcatttggcc tggtcattgg cgtcttcttt
     1261 gttcataact aggcaatcat gccagtgaaa ttttatgggt attgcttcca ctggatatta
     1321 ctaggagaca caatctctct taaaacaaaa acaaaaacaa acaaaacacc aaaagacagt
     1381 tcaagtgggg gctcacttta ttctgctcct tcccccaaag gcctccaaag ttctcacctg
     1441 tgggaatttg tcagagactt gctgctgtcc cctgaagaga actgtggcat cctggaatgg
     1501 gaagacaggg agcagggcat tttccgagtg gttaagtcag aagccctggc aaagatgtgg
     1561 ggacaaagga agaagaatga caggatgacg tacgagaagc tgagccgagc cctgaggtaa
     1621 gtgggcagca acggacgcag tgaacctgca aagacaagtt atataaaata gcggggccgg
     1681 ggaaacccac actctgttca aagcttcccc tcccccgctt catatgaaag ggtcacgagc
     1741 taaacctctt cttagtttca gatgggatta tgggtggtaa agtgacccac catccttttt
     1801 tggccttggc agcattccct gggggggatc accctaaacc caagctgtgg tgcacaccag
     1861 cacacaaacg cacgctcaac tgactgtgga gagagaggtt cagcctgtga agtgtctggg
     1921 aaggaagggt ttaagtgtaa gctgataacg ggtgtgaaaa taccagggta agtagagccc
     1981 gggctatcac ctgagagagc caggagtgtt tatatatgct gttgtttttc ttgtggtgca
     2041 aaccttctct ggatttctct cctttgcttg ctttcacaga tactactata aaacgggaat
     2101 tctggagcgg gttgaccgga ggttagtgta caaatttgga aagaacgcgc acgggtggca
     2161 ggaagagaaa ctcgttaacg gcagcggaga gggcagagga agtcttctaa catgcggtga
     2221 cgtggaggag aatcccggcc ctaggctcga gacgcgtatg gtgagcaagg gcgaggagct
     2281 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2341 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2401 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     2461 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2521 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2581 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2641 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2701 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2761 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2821 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     2881 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2941 cgggatcact ctcggcatgg acgagctgta caaggacccc aagaagaaga ggaaggtgga
     3001 ccccaagaag aagaggaagg tgtaaagatc tataacttcg tatagcatac attatacgaa
     3061 gttataagct ttctagggta ccgaagttcc tattctctag aaagtatagg aacttcctcg
     3121 aggggcgcgc ccccagctgg ttctttccgc ctcagaagcc atagagccca ccgcatcccc
     3181 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     3241 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     3301 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     3361 tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gctctagaga attgatcccc
     3421 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     3481 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     3541 ggtagccaac gctatgtcct gatagcgatc cgccacaccc agccggccac agtcgatgaa
     3601 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     3661 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     3721 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     3781 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     3841 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     3901 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     3961 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     4021 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     4081 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     4141 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     4201 atcttgttca atggccgatc ccatattggc tgcaggtcga aaggcccgga gatgaggaag
     4261 aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg
     4321 accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc
     4381 ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa
     4441 ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg
     4501 ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga
     4561 ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc
     4621 taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca
     4681 gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
     4741 agggcgcgcc gggatccgaa gttcctattc tctagaaagt ataggaactt catcgatcct
     4801 agaattcata acttcgtata gcatacatta tacgaagtta tccgcggcca ggctcatttg
     4861 atggatttct gttgttggaa acaatcagat caaactagac atttgaaagt ctccctcctc
     4921 ctcctccccc tccttcccct cctcttcttc ctccccctcc tcctcttcaa aacctacaaa
     4981 cacactgata aaatttctgc atgtctcatg cttacatttg aattcagttg ttgtctattg
     5041 gggcgatgcc atcagccctt aagcaatcgt cttcatccca agggggagga agggatggtc
     5101 ttgtggcaac ttggtgtgac actgtctcct taatgaagtg tttggagcta agggagccag
     5161 tgttatgggt gctgtttcac aagaggaccc gttgcaccat taagacacat gatcctcccg
     5221 ttccaggggt tctgagcggt cgactgaggc agcttgcctg tggttagttt ttaggaaagg
     5281 gagatgtaag acttccttgc tttagatttg aaattatcac agttatattc catagaagaa
     5341 tttttaatta aaaaaatttt agtggctaag ccactaaact gggacctaat tggatgtagc
     5401 ctaagttact aataagtctc ttaaccagat caccatttcc aaccacttag ccacagtcac
     5461 ttgatccacg gccaatcctt ctgaacttaa catccttgta gttagtcacc ttgggaattg
     5521 ctacctagat tgttaccccc ttcacctcac tggtggctat catcaggtct acagtgacct
     5581 gatcaacaga catgtgcatt aatttctaaa tcactgctgt gcctatgatt caaaccgtca
     5641 gcgtgttcag tttattgatt ctctctgagg tcagaattta ttgattctct ctgaggctaa
     5701 gacattaaac ctttaccaag cagagaacgt cctaacaagc cacgatagcc gaacacagca
     5761 tcgatctctt ctcttttctg atgaatactc aaactttcca acatattctc ttcacaaaag
     5821 taaagacagt gaatttacat caatcaacgt tcatgggtta aagtctgcac tgacatttcc
     5881 tcgtctgccg ttgcatgctg ttggcatgca aggtgttaat gacctgcaac atggtggagt
     5941 gccctgaacc ctaacttccc cagagttggg actgtctagt gaccggcact gaatagcaat
     6001 gcaggctgaa gacctccagg tttagaattt aacctcaaaa gtaatttaaa aagaaatgtg
     6061 aattactgta aaataatcta tttttggatt cgtgtgtttt tcaggtggat atagtttata
     6121 aacaatgtga ataaaaaata tttaacatgt tcaaatgtct gtgtcttgtt caattcagca
     6181 acctaggaac ctttgatggc tcattttcta gtagctggga ctttaaagag tcctccaaaa
     6241 tctaagaagt aagaatcctg actgctacct tcaggtatca taaaaatttc tactactgaa
     6301 taaggctcat aggaccaacc cgggggggtc gacgcggccg ccatggtcat agctgtttga
     6361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6421 tacattcaaa tatgtatccg ctcacgagac aataaccctg ataaatgctt caataatatt
     6481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttctggcct tttgcacgga
     8161 ccgtagagtc gagcagtgtg gttttcaaga ggaagcaaaa agcctctcca cccaggcctg
     8221 gaatgtttcc acccaatgtc gagcagtgtg gttttgcaag aggaagcaaa aagcctctcc
     8281 acccaggcct ggaatgtttc cacccaatgt cgagcaaacc ccgcccagcg tcttgtcatt
     8341 ggcgaattgg aacacgcaga tgcagtcggg gcggcgcggt cccaggtcca cttcgcatat
     8401 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
     8461 caacagcgtg ccgcacatct tggtggcgtg aaactcccgc acctcttcgg ccagcgcctt
     8521 gtagaagcgc gtatggcttc gtaccccggc catcagcacg cgtctgcgtt cgaccaggct
     8581 gcgcgttctc gcggccatag caaccgacgt acggcgttgc gccctcgccg gcagcaagaa
     8641 gccacggaag tccgcccgga gcagaaaatg cccacgctac tgcgggttta tatagacggt
     8701 ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg ttcgcgcgac
     8761 gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc ttccgagaca
     8821 atcgcgaaca tctacaccac acaacaccgc cttgaccagg gtgagatatc ggccggggac
     8881 gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc cgtgaccgac
     8941 gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc gcccccggcc
     9001 ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc ggccgcgcga
     9061 taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct catcccgccg
     9121 accttgcccg gcacaaacat cgtgttgggg gcccttccgg aggacagaca catcgaccgc
     9181 ctggccaaac gccagcgccc cggcgagcgg cttgacctgg ctatgctggc cgcgattcgc
     9241 cgcgtttacg ggctgcttgc caatacggtg cggtatctgc agggcggcgg gtcgtggcgg
     9301 gaggattggg gacagctttc ggggacggcc gtgccgcccc agggtgccga gccccagagc
     9361 aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg ggcccccgag
     9421 ttgctggccc ccaacggcga cctgtacaac gtgtttgcct gggccttgga cgtcttggcc
     9481 aaacgcctcc gtcccatgca cgtctttatc ctggattacg accaatcgcc cgccggctgc
     9541 cgggacgccc tgctgcaact tacctccggg atgatccaga cccacgtcac caccccaggc
     9601 tccataccga cgatctgcga cctggcgcgc acgtttgcac gggagatggg ggaggctaac
     9661 tgaaacacgg aaggagacaa taccggaagg aacctgcgct atgacggcaa taaaaagaca
     9721 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     9781 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     9841 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     9901 cggcaggccc tgccatagcc acgggccccg tgggttaggg acggggtccc ccatggggaa
     9961 tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagt ccacgactgg
    10021 actgagcaga cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc
    10081 gacacgaaca ccgggcgtct gtggct
//
