LOCUS       sequence-252935-       11738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 11738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH84
FEATURES             Location/Qualifiers
     source          1..11738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2674..2697,2698..2700,2701..6774)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6775..6822
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6844..7092
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7105..7125
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7141..7393
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7441..7465
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7621..8769)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(9105..9693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9185..9204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9864..10655,10656..10724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10487..10506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10725..10829)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10916..11547
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    11639..11663
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggaagc gagcccagcc
     2041 tcgggcccga ggcacctgat ggacccgcac attttcacct ccaacttcaa caacgggatc
     2101 ggcaggcaca agacgtacct gtgctatgag gttgagcgtc ttgacaatgg gaccagcgtc
     2161 aagatggatc agcacagagg gttcttgcac aaccaagcca agaatctcct ctgcgggttt
     2221 tacggccgcc acgcggagct ccgtttcttg gatctcgtcc catcactgca gctcgaccca
     2281 gcccagatct atcgcgttac atggttcatc tcgtggagcc cctgcttctc ctggggttgc
     2341 gcaggtgaag tcagagcctt cctccaggaa aatacccacg tcaggctgcg cattttcgcc
     2401 gcgagaatct atgattacga tcccctctat aaggaggccc tccagatgct ccgcgatgcc
     2461 ggtgcgcagg tcagcataat gacctatgac gagtttaagc actgctggga caccttcgtg
     2521 gaccatcagg gctgtccttt ccaaccctgg gatggactcg atgagcattc tcaggccttg
     2581 agcggcaggc tgagagccat cttgcaaaat caaggtaatt ctggctctga aacaccaggt
     2641 acttctgagt ccgcaactcc tgagtcattg aaggacaaga aatactccat tgggttggcc
     2701 ataggcacca actccgttgg ctgggcagtg atcaccgacg aatacaaggt cccgagcaaa
     2761 aagtttaagg tgctgggcaa tactgatcgt cactcaatca agaagaacct gattggagcc
     2821 ctgcttttcg actctgggga aacggctgag gcaacgcgct tgaagaggac cgcgcgcagg
     2881 cggtataccc gtaggaagaa tcgtatctgc taccttcagg agatcttttc taatgaaatg
     2941 gctaaggttg atgatagctt cttccaccgc ctggaggaat cttttctcgt cgaggaggac
     3001 aagaaacacg agcgccaccc aatctttgga aacatcgtcg acgaggttgc ctatcatgaa
     3061 aaatacccaa ctatatatca tctccgtaag aagctcgtcg acagcacaga caaagcagac
     3121 ttgaggctga tatacttggc attggctcac atgataaaat tccgtggcca ctttcttatc
     3181 gagggagatc tgaatccgga caacagcgac gttgataaac tctttatcca gctggttcag
     3241 acctacaatc aattgttcga ggagaacccg atcaatgcgt ctggcgtcga cgcaaaggcc
     3301 atcctgtccg cgcggctcag caaaagccgc agattggaga atctgatcgc acagcttccg
     3361 ggtgagaaga aaaacggcct cttcggcaac ttgatcgccc tctctctcgg ccttacccct
     3421 aacttcaagt cgaacttcga cctcgccgag gacgccaagc ttcagctctc aaaggacacc
     3481 tacgacgacg acctcgataa ccttctcgca cagatcggcg atcaatacgc cgatctcttc
     3541 ctcgcggcca agaacctgag cgatgcgatt ctgttgtccg atatcctcag agtcaacaca
     3601 gagatcacca aggctcccct ctccgcgtca atgattaagc gttatgatga gcaccatcag
     3661 gatctgacac tgctcaaggc tctcgtgcgt cagcagctcc ccgagaagta taaggagatc
     3721 ttcttcgacc aaagcaagaa tggctacgcc gggtatattg acggcggcgc gtcccaagaa
     3781 gaattttata agtttatcaa gccaatcctg gaaaagatgg acggaacgga agaactgctg
     3841 gtgaaattga acagggagga cctcctcagg aaacaacgca catttgataa cgggagcatc
     3901 ccacatcaaa tacatctggg tgagcttcac gccatcctgc gccggcagga ggacttctac
     3961 cccttcctga aggacaaccg ggagaagatc gaaaagatac tcaccttcag aatcccctac
     4021 tacgtcggcc cactcgccag gggcaactct aggttcgcct ggatgactag gaagtcagaa
     4081 gaaaccatca ctccctggaa cttcgaggag gtggtggata aaggcgccag cgcccagagc
     4141 ttcatcgaga ggatgaccaa tttcgataag aatttgccaa acgaaaaggt gttgccaaag
     4201 cactccctgc tttacgaata cttcacggtc tacaacgagc tgaccaaagt gaagtacgtg
     4261 acagagggaa tgaggaagcc agccttcctg tctggggagc aaaagaaggc cattgtggac
     4321 ctcttgttta agacaaatcg caaagtgacg gtgaagcaac tcaaggagga ttactttaaa
     4381 aagattgagt gtttcgactc cgtggagatc agcggggtcg aggacaggtt taacgccagc
     4441 ctggggacat atcacgacct gctgaaaatc attaaggaca aggacttcct ggacaacgag
     4501 gagaatgagg acattctcga ggatatcgtg ctcacgctga ctctcttcga ggacagagag
     4561 atgatcgagg agagactcaa gacctacgct catctgttcg acgacaaagt catgaaacag
     4621 cttaagcgta gacgttacac cggctgggga cgcctgtccc gtaagctgat taatgggatt
     4681 cgcgataagc agagcggcaa gacaatactc gatttcttga agtccgacgg tttcgccaat
     4741 aggaacttca tgcagctcat ccatgatgat tcgctcacct ttaaggagga tattcagaag
     4801 gcgcaagttt ctggccaagg cgatagcctc cacgaacata tcgccaacct ggcgggcagc
     4861 cccgcaatca aaaagggcat cctgcaaacc gttaaggttg tggacgaact ggttaaggtc
     4921 atgggcagac ataagccgga aaacatagtc attgaaatgg ctcgcgagaa ccagacaact
     4981 cagaagggcc aaaagaattc gcgggagagg atgaagagaa ttgaggaggg aatcaaggag
     5041 ctcggatcgc agattctcaa ggagcaccct gtggagaata ctcaacttca gaacgaaaaa
     5101 ctgtacttgt actacttgca gaacgggaga gatatgtacg ttgatcagga gcttgacatc
     5161 aaccgcctga gcgactacga tgtggaccac atagtcccac agtctttcct gaaggacgac
     5221 agcatcgata ataaggtctt gacccgcagc gacaagaatc gggggaaaag cgataacgtt
     5281 ccttcggagg aggtcgtcaa gaagatgaag aattactgga ggcaattgtt gaatgccaag
     5341 cttatcacgc aaagaaagtt cgacaacctt acgaaggctg agcgcggggg tctttccgaa
     5401 ttggacaagg cggggttcat aaagcgccag ctcgtggaga ctagacaaat caccaagcac
     5461 gtggcccaga ttctggactc acggatgaac accaagtacg acgagaacga taaactcatc
     5521 cgggaggtca aggttatcac cctcaagagc aagctggtgt ccgacttcag gaaggatttt
     5581 caattctata aggtccggga aattaataat taccaccacg cccacgatgc gtaccttaac
     5641 gcggtggtgg gcacggctct gatcaagaag tacccaaagc tggagtccga gtttgtttat
     5701 ggtgactata aggtgtacga tgtccgcaaa atgattgcca agagcgagca ggaaataggc
     5761 aaggcgacag ccaagtactt cttctactca aatatcatga atttctttaa gacagaaatc
     5821 acgctggcca atggcgaaat tagaaagcgt ccgctgattg agactaacgg agagactggc
     5881 gagatagttt gggacaaagg ccgcgatttc gctaccgtca gaaaggtgct gtctatgcca
     5941 caggtcaaca tcgtcaagaa aaccgaagtc cagaccggcg gcttctctaa ggagtctatc
     6001 ctgcctaaga ggaactccga taagttgatt gctcgcaaga aggactggga cccaaagaag
     6061 tacggtggat ttgactcccc gacagtcgct tacagcgtcc ttgtcgttgc caaggttgag
     6121 aaaggcaaga gcaagaagct caagagcgtg aaggagctgt tggggatcac cattatggag
     6181 aggagctcct tcgagaagaa cccgattgac ttcttggaag caaagggcta caaagaggtt
     6241 aagaaggacc tcatcatcaa gttgccaaag tactcccttt tcgagctcga gaacgggcgc
     6301 aagagaatgc tcgcctccgc aggggaactc cagaagggca acgaattggc ccttccttca
     6361 aaatacgtga attttctgta cctggcgtcc cattacgaaa agctgaaagg gtccccggag
     6421 gacaacgaac aaaagcaact gttcgtcgag caacataagc actatcttga cgaaataatc
     6481 gagcaaatct ccgagttctc taagagggtc atccttgctg acgccaactt ggacaaggtc
     6541 ctgtcagcct acaacaagca ccgggacaag ccgatcagag agcaagctga gaatattatt
     6601 cacctcttca ccttgaccaa cttgggcgcc cccgcggcgt tcaagtactt tgacaccacg
     6661 atcgatcgta agcggtacac ctccaccaaa gaagttctcg acgccaccct tatccaccaa
     6721 tcaatcactg gcctttacga aacgaggatc gatctgagcc aattgggagg ggataaacgg
     6781 cccgcagcta ccaagaaggc ggggcaggca aaaaagaaga aaaccaggga cagcggcgga
     6841 agcactaacc ttagcgacat catcgaaaag gagacaggaa aacagctcgt tatccaagaa
     6901 agcatcctta tgctccctga ggaagttgag gaagttatcg gaaacaagcc tgagagcgac
     6961 atcctggtgc acacggcata tgacgagtcc accgacgaga acgtgatgtt gctcactagc
     7021 gacgcacccg aatacaagcc gtgggctctt gtgatccagg actccaatgg ggagaacaag
     7081 attaagatgc tctctggcgg ttccccgaag aagaaacgca aggtgtgagc ttgtcaagca
     7141 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     7201 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     7261 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     7321 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     7381 atgttactag atcgacgcta ctattgtctt ctgcacgaag tggtttaaac tatcagtgtt
     7441 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     7501 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     7561 gggttcccca gatcaggcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc
     7621 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
     7681 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
     7741 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
     7801 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
     7861 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
     7921 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
     7981 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
     8041 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
     8101 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
     8161 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
     8221 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
     8281 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
     8341 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
     8401 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt
     8461 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
     8521 cgcctcctgt tcaagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
     8581 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc
     8641 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
     8701 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
     8761 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta
     8821 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa
     8881 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg
     8941 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
     9001 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
     9061 ctaggatctg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg
    10021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10741 tattattgaa gcatttatca aggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10801 tagaaaaata aacaaatagg ggttccgcgc acgaattggc cagcgctgcc atttttgggg
    10861 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    10921 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    10981 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    11041 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    11101 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    11161 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    11221 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    11281 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    11341 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    11401 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca
    11461 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg
    11521 gcgagggcaa ccagcccggt gagcgtcgca aaggagatcc tgatctgact gatgggctgc
    11581 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    11641 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    11701 cgtttttaat gtactggggt ggatgcagtg ggccccac
//
