LOCUS       sequence-252936-       13823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 13823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH82
FEATURES             Location/Qualifiers
     source          1..13823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             789..809
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5671..5680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             5696..5704
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      7986..8238
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8286..8310
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(8466..9614)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(9950..10538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10030..10049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10709..11500,11501..11569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11332..11351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11570..11674)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11761..12392
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    12484..12508
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg
       61 cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct
      121 tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag
      181 aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata
      241 catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac
      301 atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga
      361 tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca
      421 aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt
      481 tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt
      541 ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt
      601 acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga
      661 tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat acgctattta
      721 tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag
      781 gaatggcccc aaagaagaag cgcaaggtga tggataagaa atactcaatt ggtttggcaa
      841 ttggcacaaa ctcggtgggc tgggccgtga ttactgacga atacaaggtg ccatcaaaga
      901 agttcaaggt gctcggcaat actgaccgcc actctatcaa gaagaatctc atcggcgcat
      961 tgctcttcga ttcgggcgag acagcagagg ctactaggct gaagaggacc gcaaggcgca
     1021 ggtacacccg ccgcaagaat cgcatatgct accttcaaga gatcttctca aacgagatgg
     1081 ccaaggtgga cgactccttc ttccataggc tcgaagagtc ctttctggtt gaagaggata
     1141 agaagcacga gaggcatcct atattcggca acatcgtgga cgaggtggcc tatcatgaga
     1201 agtaccctac gatctaccat ctccgcaaga agctggttga cagcaccgat aaagccgatt
     1261 tgcgcctgat ctacctggca ctcgcacata tgatcaagtt ccgcgggcat ttccttatcg
     1321 agggagatct caaccccgat aactccgacg tggacaagct cttcattcag cttgtgcaaa
     1381 cctataatca gctgttcgag gagaatccaa tcaacgcgtc tggtgttgat gcaaaggcga
     1441 tcctgtccgc gcgcctgtca aaatcacgcc ggctggaaaa cttgattgcg caacttccag
     1501 gtgagaagaa gaacggtttg ttcggcaatt tgatcgcact cagcctcgga ctgaccccca
     1561 acttcaagtc caacttcgat ctggccgagg acgccaaact ccagctttcc aaggacacct
     1621 acgatgatga cctcgataac cttttggctc agattgggga ccagtacgca gatctgttcc
     1681 ttgcggcgaa gaatctctca gacgccatcc tgctttccga tatcctgagg gtcaacaccg
     1741 agatcaccaa ggcgccgctg tccgcatcca tgatcaagcg ctacgatgaa caccaccagg
     1801 atctcaccct cctcaaggcc ctggtgcgcc agcaattgcc tgaaaagtac aaggagatct
     1861 ttttcgatca gtccaagaat ggctacgcgg gctacattga cggcggagcg agccaagagg
     1921 aattctataa gttcatcaag ccgattcttg agaagatgga tggcaccgag gagcttttgg
     1981 tcaaactgaa cagagaggac ttgttgagaa agcagagaac cttcgacaat ggctctatcc
     2041 cccatcaaat tcacctcgga gagctgcacg caatcctccg tcgccaggag gacttctatc
     2101 cattccttaa agataacagg gaaaaaatcg aaaagatcct cacgttccgc attccatact
     2161 acgtgggtcc actcgcgcgg ggtaatagca gatttgcgtg gatgacccgg aagtcggaag
     2221 agacaatcac tccatggaat ttcgaggagg ttgtcgataa gggtgcgagc gcccaatcct
     2281 ttattgaacg tatgaccaac ttcgacaaga accttcccaa cgaaaaagtg ctcccaaagc
     2341 attccctgtt gtatgagtac ttcaccgttt ataatgaatt gaccaaagtt aaatacgtca
     2401 cggagggcat gcggaagccg gctttcctct cgggagagca aaagaaggca attgtcgatc
     2461 tgcttttcaa aacgaaccgt aaagtcaccg tgaagcaact caaagaggac tatttcaaga
     2521 agatcgaatg cttcgactcg gtcgaaatta gcggtgtcga ggatcggttc aacgcctcgc
     2581 tgggcactta ccacgatctc ttgaagatca taaaggacaa ggacttcctg gataacgagg
     2641 agaacgagga tattttggaa gatatagtgt tgacgctgac cctttttgag gatcgcgaaa
     2701 tgatagaaga aagacttaag acatacgccc accttttcga cgacaaggtt atgaaacaac
     2761 ttaagcggag gaggtacacc ggttggggga gactgtctcg taagttgatc aatggcattc
     2821 gcgacaagca atccggcaag accatcctgg actttttgaa gtccgacggt tttgcgaatc
     2881 gcaattttat gcaactgatc cacgacgact cactcacttt caaagaggac attcaaaaag
     2941 cgcaagtttc aggccaaggc gactcacttc atgagcacat agcaaatctt gcaggctcac
     3001 cagccatcaa aaaaggcatt ctgcaaactg tcaaggtggt tgacgagctc gtcaaggtta
     3061 tgggccggca taagcccgaa aacatcgtta tcgagatggc gcgggagaat cagaccactc
     3121 aaaaggggca gaagaactcc agagaacgga tgaagcgtat agaggaaggg atcaaggagc
     3181 tgggttccca gattctcaag gagcatccag ttgagaacac acaactccag aatgagaagc
     3241 tctatttgta ctacctgcaa aacggcaggg acatgtacgt tgaccaggaa ctggacatta
     3301 accgcttgtc agattacgac gttgaccaca tcgtgcccca gagctttctc gcggatgatt
     3361 caatcgataa caaggtgctg acccgcagcg acaagaaccg tggtaagagc gataatgtgc
     3421 cctcagaaga agtcgttaag aagatgaaaa attactggag gcaactcctg aacgctaagt
     3481 tgatcacgca acgtaagttc gacaacctga ccaaggccga gaggggcggc ctgtccgagc
     3541 ttgacaaagc tggttttata aagcgccagt tggtggagac tcgccaaata acaaagcacg
     3601 tggcacaaat ccttgactcc aggatgaata ccaagtacga cgagaacgac aagctgatcc
     3661 gcgaggtcaa agtgataact cttaagtcga agctggtgtc tgacttccgg aaggacttcc
     3721 agttttataa ggtgagggag atcaacaact accatcacgc ccacgatgca tacctgaacg
     3781 cggttgtggg cactgccctg atcaagaaat atccagccct cgaatctgag ttcgtctacg
     3841 gcgactacaa agtttacgac gtcaggaaga tgatcgcgaa gtctgaacaa gagatcggca
     3901 aggcgaccgc taagtatttc ttttactcga acatcatgaa cttcttcaag accgaaatta
     3961 cactggcgaa cggcgagatt cgcaaggccc cactcatcga aaccaacggt gagacaggtg
     4021 agatcgtctg ggataagggc agggacttcg cgaccgtgag gaaggtcctg tccatgccac
     4081 aagtgaacat agtgaagaaa accgaggtgc aaacaggagg gttctcaaag gagagcattc
     4141 tccctaagcg gaattccgac aagctgattg cccggaagaa ggactgggat cccaagaaat
     4201 atggaggttt cgactccccg acggtggcgt attctgtcct cgtcgtggcc aaggttgaga
     4261 aaggaaagag caagaagctc aagagcgtca aggagctcct cgggatcacc attatggagc
     4321 ggtcatcatt cgaaaagaac ccgatcgatt ttctggaagc caagggctac aaggaggtca
     4381 agaaggatct cataattaag ctcccaaagt actccctgtt cgagcttgaa aacgggcgga
     4441 agcgcatgct ggcctccgcc ggagagctcc agaagggaaa cgagctcgcc ctgccttcta
     4501 aatacgtcaa ctttctgtat cttgcctcac actacgagaa gctgaagggc tcaccggagg
     4561 ataacgagca aaagcagctt tttgttgaac agcacaagca ctaccttgac gagatcattg
     4621 agcaaatttc ggagttcagc aagagagtca tcctcgcaga cgccaatctt gacaaggttc
     4681 tgtcagcgta taacaagcac agagacaagc cgatacgtga gcaagctgag aacataattc
     4741 acctcttcac gcttactaat ttgggggctc cggcggcctt taagtacttc gacactacca
     4801 tagacaggaa aagatacacc tctaccaagg aggtccttga cgctacactt atccaccaat
     4861 caatcacggg gttgtacgag actcggatcg accttagcca gttgggcggc gatggcagct
     4921 ccgaaacgcc tggaacgtcc gagagcgcca cccccgaagg cgggtccgga ggatccgggt
     4981 ccgtccagat cccccagaac ccgctgatac tcgtggacgg aagctcgtac ctgtacaggg
     5041 cataccatgc gtttccacca cttacgaact ctgctggcga accaacaggg gccatgtatg
     5101 gcgtcttgaa tatgctccgg tccctgatca tgcaatacaa gccaacccat gcagctgtgg
     5161 tgttcgacgc gaaaggcaag actttccggg acgaactctt tgagcattat aagagccata
     5221 gaccacccat gccggacgat ctgcgcgccc agatagagcc actgcacgcc atggttaagg
     5281 cgatgggcct tccccttctc gcggttagcg gggtggaggc agacgacgtc attggaacgc
     5341 tcgctagaga ggccgaaaag gccggcaggc cggttctcat cagcactggg gataaggata
     5401 tggctcagct cgtgacacca aacataactc tgatcaacac catgaccaac actatcctcg
     5461 gtccagaaga agttgtgaat aagtacggcg tcccgcctga gctcatcatt gacttccttg
     5521 cgctcatggg agactcatcg gacaatatac ccggcgtgcc gggagtcggt gaaaagaccg
     5581 cccaagcgct cttgcaggga ttgggggggc tggacacctt gtacgcggag ccagagaaga
     5641 tcgcaggttt gtcgttccgc ggcgccaaga ccatggcggc caagcttgaa cagaataagg
     5701 aggtggccta tctctcctat caactcgcca cgataaagac cgacgtcgag ctcgagctca
     5761 cctgtgagca actcgaggtt cagcaaccgg cggccgagga gctgctcggc ctgtttaaga
     5821 agtacgagtt caagcgctgg acagcggatg ttgaggcagg caaatggctt caggctaagg
     5881 gagctaagcc cgcggccaag ccacaagaaa cttccgtggc cgacgaagcg ccggaggtca
     5941 ctgcgaccgt gatatcgtac gacaattacg tcacgattct tgatgaggag acactcaagg
     6001 cgtggattgc aaagttggag aaggccccgg tgtttgcatt tgatactgaa accgactctc
     6061 tggacaatat ttcggcgaac ctcgtcggac tgtcgtttgc aattgagccg ggcgtggctg
     6121 cgtacatccc agtggcgcat gactatttgg acgcccccga tcaaatcagc cgggaaagag
     6181 cgcttgaact cctcaagcca cttctcgagg atgagaaagc cctgaaggtg gggcaaaatc
     6241 tcaagtacgc ccgtgggatc ttggccaact acggcatcga gctccgcggg atcgcattcg
     6301 acaccatgtt ggagtcctac attctcaact ccgttgcggg caggcatgat atggactccc
     6361 tggccgaaag atggctgaag cacaagacga tcacattcga agagattgca ggcaaaggca
     6421 agaaccagtt gacttttaac caaatcgctc tggaggaggc gggccggtac gccgcagagg
     6481 acgctgatgt gacccttcaa ctccacctca agatgtggcc ggacctccaa aagcataagg
     6541 ggccattgaa cgtgttcgag aacattgaaa tgccgcttgt tccagtgctc agcaggattg
     6601 agcgcaacgg ggttaaaatt gaccccaagg tgcttcacaa tcactccgaa gagctgaccc
     6661 ttcggctggc agagcttgaa aaaaaagcca ctgagacatt tggctcctgg tatcaaccga
     6721 agggcggcac agaaatgttc tgccaccccc ggacggggaa gccgctgcca aagtacccac
     6781 ggatcaagac tcccaaagtt ggcgggatct ttaagaagcc gaagaacaag gcccagcgcg
     6841 agggtcgtga gccttgcgaa ttggacactc gtgagtacgt tgcaggcgcc ccatacacgc
     6901 ccgtcgagca cgtcgttttt aacttgtcat ccaccaagca gctgcagacc atcctcttcg
     6961 agaagcaggg aattaagccg ctcaagaaaa cgcccggcgg cgccccgtcc acttctgagg
     7021 aagtcctgga ggagcttgcc ctggactacc cactcccgaa ggtgatcttg gagtacaggg
     7081 gactcgccaa attgaagagc acctacacgg acaagctccc tctcatgatc aatcctaaaa
     7141 cgggccgcgt ccatacgagc taccaccaag cagtgaccgc gacaggaagg ctgtcgtcaa
     7201 ctgatccaaa tcttcaaaat atcccagtca gaaacgagga aggacggaga atccggcagg
     7261 cgttcattgc tccggaggat tacgtcatcg tttctgcaga ctactctcaa aatgagctga
     7321 ggatcatggc tcatttgtcc cgcgacaagg gcctgctgac ggccttcgcg gagggcaagg
     7381 acatccaccg tgccaccgcc gcagaggttt tcggcctccc cttggagaca gtgacatcag
     7441 agcaacgccg gtcggccaag cgtattaatt tcggcctgat atacggcatg tccgcctttg
     7501 gcctcgctcg gcagctcaac ataccaagga aggaggccca gaagtacatg gacttgtatt
     7561 tcgaacgtta ccccggcgtc ctcgagtata tggagcgtac tcgggcccaa gctaaggagc
     7621 aggggtatgt cgagacactg gatgggcgtc gcctctatct gcccgacatc aagtcatcaa
     7681 atggcgcccg tagagcggcg gccgaacgtg cagccatcaa cgcacctatg caaggaactg
     7741 ccgcggatat aattaaacgg gcaatgatcg ccgtcgatgc ctggctccaa gcggagcaac
     7801 caagggttag gatgatcatg caggtccacg atgagcttgt ctttgaggtc cacaaggatg
     7861 atgtcgacgc agtcgccaag cagattcacc agttgatgga gaattgcacc cgcctggacg
     7921 tccccctcct ggtcgaggtg gggtcgggcg agaactggga tcaggcgcat tgagcttgtc
     7981 aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     8041 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     8101 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     8161 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     8221 catctatgtt actagatcga cgctactatt gtcttctgca cgaagtggtt taaactatca
     8281 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     8341 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     8401 ccacagggtt ccccagatca ggcgctggct gctgaacccc cagccggaac tgaccccaca
     8461 aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg
     8521 ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg
     8581 gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc
     8641 gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat
     8701 atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc
     8761 tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc
     8821 gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac
     8881 aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa
     8941 agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac
     9001 acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc
     9061 ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc
     9121 ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg
     9181 tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg
     9241 tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg
     9301 gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa
     9361 cctgccgcct cctgttcaag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg
     9421 gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc
     9481 gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt
     9541 tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca
     9601 aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc
     9661 ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca
     9721 atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg
     9781 ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag
     9841 ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc
     9901 cacatctagg atctgccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9961 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10021 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10081 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10141 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10201 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10261 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10321 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10381 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10441 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10501 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10561 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10621 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10681 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10741 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10801 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct
    10861 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10921 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10981 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11041 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11101 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11161 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11221 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11281 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11341 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11401 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11461 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11521 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11581 ttcaatatta ttgaagcatt tatcaaggtt attgtctcat gagcggatac atatttgaat
    11641 gtatttagaa aaataaacaa ataggggttc cgcgcacgaa ttggccagcg ctgccatttt
    11701 tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt
    11761 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
    11821 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
    11881 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
    11941 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
    12001 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
    12061 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
    12121 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
    12181 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
    12241 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc
    12301 tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc
    12361 cagcggcgag ggcaaccagc ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg
    12421 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
    12481 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
    12541 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtga agacaagcaa
    12601 gaattcaagc ttggaggtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca
    12661 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
    12721 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
    12781 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
    12841 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
    12901 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
    12961 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt
    13021 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa
    13081 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa
    13141 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta
    13201 aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca
    13261 agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc
    13321 tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg
    13381 tgagccggca cggcaggcgg cctcctcctc ctctcacggc acggcagcta cgggggattc
    13441 ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc
    13501 cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc
    13561 cccaaatcca cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc
    13621 cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt
    13681 ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca
    13741 cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg
    13801 ggaatcctgg gatggctcta gcc
//
