LOCUS       sequence-252939-        9623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH52
FEATURES             Location/Qualifiers
     source          1..9623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        438..783
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     726..746
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             874..4977
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             874..4977
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4990..5010
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5026..5278
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5326..5350
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5506..6654)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(6990..7578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7070..7089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7749..8540,8541..8609))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8372..8391
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8610..8714)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8801..9432
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9524..9548
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcaaca tggtggagca cgacactctg
       61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      361 gataacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca
      421 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga
      481 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      541 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt
      601 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc
      661 acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      781 acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac
      841 aacattacaa ttacatttac aattatcgat acaatggata agaagtactc tatcggactc
      901 gatatcggaa ctaactctgt gggatgggct gtgatcaccg atgagtacaa ggtgccatct
      961 aagaagttca aggttctcgg aaacaccgat aggcactcta tcaagaaaaa ccttatcggt
     1021 gctctcctct tcgattctgg tgaaactgct gaggctacca gactcaagag aaccgctaga
     1081 agaaggtaca ccagaagaaa gaacaggatc tgctacctcc aagagatctt ctctaacgag
     1141 atggctaaag tggatgattc attcttccac aggctcgaag agtcattcct cgtggaagaa
     1201 gataagaagc acgagaggca ccctatcttc ggaaacatcg ttgatgaggt ggcataccac
     1261 gagaagtacc ctactatcta ccacctcaga aagaagctcg ttgattctac tgataaggct
     1321 gatctcaggc tcatctacct cgctctcgct cacatgatca agttcagagg acacttcctc
     1381 atcgagggtg atctcaaccc tgataactct gatgtggata agttgttcat ccagctcgtg
     1441 cagacctaca accagctttt cgaagagaac cctatcaacg cttcaggtgt ggatgctaag
     1501 gctatcctct ctgctaggct ctctaagtca agaaggcttg agaacctcat tgctcagctc
     1561 cctggtgaga agaagaacgg acttttcgga aacttgatcg ctctctctct cggactcacc
     1621 cctaacttca agtctaactt cgatctcgct gaggatgcaa agctccagct ctcaaaggat
     1681 acctacgatg atgatctcga taacctcctc gctcagatcg gagatcagta cgctgatttg
     1741 ttcctcgctg ctaagaacct ctctgatgct atcctcctca gtgatatcct cagagtgaac
     1801 accgagatca ccaaggctcc actctcagct tctatgatca agagatacga tgagcaccac
     1861 caggatctca cacttctcaa ggctcttgtt agacagcagc tcccagagaa gtacaaagag
     1921 attttcttcg atcagtctaa gaacggatac gctggttaca tcgatggtgg tgcatctcaa
     1981 gaagagttct acaagttcat caagcctatc ctcgagaaga tggatggaac cgaggaactc
     2041 ctcgtgaagc tcaatagaga ggatcttctc agaaagcaga ggaccttcga taacggatct
     2101 atccctcatc agatccacct cggagagttg cacgctatcc ttagaaggca agaggatttc
     2161 tacccattcc tcaaggataa cagggaaaag attgagaaga ttctcacctt cagaatccct
     2221 tactacgtgg gacctctcgc tagaggaaac tcaagattcg cttggatgac cagaaagtct
     2281 gaggaaacca tcaccccttg gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag
     2341 tctttcatcg agaggatgac caacttcgat aagaaccttc caaacgagaa ggtgctccct
     2401 aagcactctt tgctctacga gtacttcacc gtgtacaacg agttgaccaa ggttaagtac
     2461 gtgaccgagg gaatgaggaa gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt
     2521 gatctcttgt tcaagaccaa cagaaaggtg accgtgaagc agctcaaaga ggattacttc
     2581 aagaaaatcg agtgcttcga ttcagttgag atttctggtg ttgaggatag gttcaacgca
     2641 tctctcggaa cctaccacga tctcctcaag atcattaagg ataaggattt cttggataac
     2701 gaggaaaacg aggatatctt ggaggatatc gttcttaccc tcaccctctt tgaagataga
     2761 gagatgattg aagaaaggct caagacctac gctcatctct tcgatgataa ggtgatgaag
     2821 cagttgaaga gaagaagata cactggttgg ggaaggctct caagaaagct cattaacgga
     2881 atcagggata agcagtctgg aaagacaatc cttgatttcc tcaagtctga tggattcgct
     2941 aacagaaact tcatgcagct catccacgat gattctctca cctttaaaga ggatatccag
     3001 aaggctcagg tttcaggaca gggtgatagt ctccatgagc atatcgctaa cctcgctgga
     3061 tctcctgcaa tcaagaaggg aatcctccag actgtgaagg ttgtggatga gttggtgaag
     3121 gtgatgggaa ggcataagcc tgagaacatc gtgatcgaaa tggctagaga gaaccagacc
     3181 actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga aggtatcaaa
     3241 gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct ccagaatgag
     3301 aagctctacc tctactacct ccagaacgga agggatatgt atgtggatca agagttggat
     3361 atcaacaggc tctctgatta cgatgttgat catatcgtgc cacagtcatt cttgaaggat
     3421 gattctatcg ataacaaggt gctcaccagg tctgataaga acaggggtaa gagtgataac
     3481 gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct cctcaacgct
     3541 aagctcatca ctcagagaaa gttcgataac ttgactaagg ctgagagggg aggactctct
     3601 gaattggata aggcaggatt catcaagagg cagcttgtgg aaaccaggca gatcactaag
     3661 cacgttgcac agatcctcga ttctaggatg aacaccaagt acgatgagaa cgataagttg
     3721 atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt cagaaaggat
     3781 ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga tgcttacctt
     3841 aacgctgttg ttggaaccgc tctcatcaag aagtatccta agctcgagtc agagttcgtg
     3901 tacggtgatt acaaggtgta cgatgtgagg aagatgatcg ctaagtctga gcaagagatc
     3961 ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt caagaccgag
     4021 attaccctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa cggtgaaaca
     4081 ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt gctctctatg
     4141 ccacaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc taaagagtct
     4201 atcctcccta agaggaactc tgataagctc attgctagga agaaggattg ggaccctaag
     4261 aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt
     4321 gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat cactatcatg
     4381 gaaaggtcat ctttcgagaa gaacccaatc gatttcctcg aggctaaggg atacaaagag
     4441 gttaagaagg atctcatcat caagctccca aagtactcac tcttcgaact cgagaacggt
     4501 agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct tgctctccca
     4561 tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa gggatctcca
     4621 gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt ggatgagatc
     4681 atcgagcaga tctctgagtt ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag
     4741 gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc agagaacatc
     4801 atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta cttcgataca
     4861 accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac cctcatccat
     4921 cagtctatca ctggactcta cgagactagg atcgatctct cacagctcgg tggtgattca
     4981 agggctgatc ctaagaagaa gaggaaggtt tgagcttgtc aagcagatcg ttcaaacatt
     5041 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     5101 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     5161 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     5221 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga
     5281 cgctactatt gtcttctgca cgaagtggtt taaactatca gtgtttgaca ggatatattg
     5341 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     5401 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt ccccagatca
     5461 ggcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
     5521 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
     5581 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag
     5641 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
     5701 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
     5761 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
     5821 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
     5881 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
     5941 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
     6001 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
     6061 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
     6121 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
     6181 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
     6241 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
     6301 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
     6361 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcaag
     6421 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
     6481 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt
     6541 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
     6601 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
     6661 tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc
     6721 gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac
     6781 cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga
     6841 ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa
     6901 gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg atctgccagg
     6961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7861 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     7921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8641 tatcaaggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8701 ataggggttc cgcgcacgaa ttggccagcg ctgccatttt tggggtgagg ccgttcgcgg
     8761 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
     8821 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
     8881 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
     8941 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
     9001 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
     9061 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
     9121 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
     9181 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
     9241 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
     9301 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
     9361 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
     9421 ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg gctgcctgta tcgagtggtg
     9481 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
     9541 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
     9601 ggggtggatg cagtgggccc cac
//
