LOCUS       sequence-252940-        9839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH53
FEATURES             Location/Qualifiers
     source          1..9839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        438..783
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     726..746
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             874..4977
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             874..4977
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4990..5010
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5542..5566
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5722..6870)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(7206..7794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7286..7305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7965..8756,8757..8825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8588..8607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8826..8930)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9017..9648
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9740..9764
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcaaca tggtggagca cgacactctg
       61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      361 gataacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca
      421 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga
      481 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      541 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt
      601 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc
      661 acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      781 acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac
      841 aacattacaa ttacatttac aattatcgat acaatggata agaagtactc tatcggactc
      901 gatatcggaa ctaactctgt gggatgggct gtgatcaccg atgagtacaa ggtgccatct
      961 aagaagttca aggttctcgg aaacaccgat aggcactcta tcaagaaaaa ccttatcggt
     1021 gctctcctct tcgattctgg tgaaactgct gaggctacca gactcaagag aaccgctaga
     1081 agaaggtaca ccagaagaaa gaacaggatc tgctacctcc aagagatctt ctctaacgag
     1141 atggctaaag tggatgattc attcttccac aggctcgaag agtcattcct cgtggaagaa
     1201 gataagaagc acgagaggca ccctatcttc ggaaacatcg ttgatgaggt ggcataccac
     1261 gagaagtacc ctactatcta ccacctcaga aagaagctcg ttgattctac tgataaggct
     1321 gatctcaggc tcatctacct cgctctcgct cacatgatca agttcagagg acacttcctc
     1381 atcgagggtg atctcaaccc tgataactct gatgtggata agttgttcat ccagctcgtg
     1441 cagacctaca accagctttt cgaagagaac cctatcaacg cttcaggtgt ggatgctaag
     1501 gctatcctct ctgctaggct ctctaagtca agaaggcttg agaacctcat tgctcagctc
     1561 cctggtgaga agaagaacgg acttttcgga aacttgatcg ctctctctct cggactcacc
     1621 cctaacttca agtctaactt cgatctcgct gaggatgcaa agctccagct ctcaaaggat
     1681 acctacgatg atgatctcga taacctcctc gctcagatcg gagatcagta cgctgatttg
     1741 ttcctcgctg ctaagaacct ctctgatgct atcctcctca gtgatatcct cagagtgaac
     1801 accgagatca ccaaggctcc actctcagct tctatgatca agagatacga tgagcaccac
     1861 caggatctca cacttctcaa ggctcttgtt agacagcagc tcccagagaa gtacaaagag
     1921 attttcttcg atcagtctaa gaacggatac gctggttaca tcgatggtgg tgcatctcaa
     1981 gaagagttct acaagttcat caagcctatc ctcgagaaga tggatggaac cgaggaactc
     2041 ctcgtgaagc tcaatagaga ggatcttctc agaaagcaga ggaccttcga taacggatct
     2101 atccctcatc agatccacct cggagagttg cacgctatcc ttagaaggca agaggatttc
     2161 tacccattcc tcaaggataa cagggaaaag attgagaaga ttctcacctt cagaatccct
     2221 tactacgtgg gacctctcgc tagaggaaac tcaagattcg cttggatgac cagaaagtct
     2281 gaggaaacca tcaccccttg gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag
     2341 tctttcatcg agaggatgac caacttcgat aagaaccttc caaacgagaa ggtgctccct
     2401 aagcactctt tgctctacga gtacttcacc gtgtacaacg agttgaccaa ggttaagtac
     2461 gtgaccgagg gaatgaggaa gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt
     2521 gatctcttgt tcaagaccaa cagaaaggtg accgtgaagc agctcaaaga ggattacttc
     2581 aagaaaatcg agtgcttcga ttcagttgag atttctggtg ttgaggatag gttcaacgca
     2641 tctctcggaa cctaccacga tctcctcaag atcattaagg ataaggattt cttggataac
     2701 gaggaaaacg aggatatctt ggaggatatc gttcttaccc tcaccctctt tgaagataga
     2761 gagatgattg aagaaaggct caagacctac gctcatctct tcgatgataa ggtgatgaag
     2821 cagttgaaga gaagaagata cactggttgg ggaaggctct caagaaagct cattaacgga
     2881 atcagggata agcagtctgg aaagacaatc cttgatttcc tcaagtctga tggattcgct
     2941 aacagaaact tcatgcagct catccacgat gattctctca cctttaaaga ggatatccag
     3001 aaggctcagg tttcaggaca gggtgatagt ctccatgagc atatcgctaa cctcgctgga
     3061 tctcctgcaa tcaagaaggg aatcctccag actgtgaagg ttgtggatga gttggtgaag
     3121 gtgatgggaa ggcataagcc tgagaacatc gtgatcgaaa tggctagaga gaaccagacc
     3181 actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga aggtatcaaa
     3241 gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct ccagaatgag
     3301 aagctctacc tctactacct ccagaacgga agggatatgt atgtggatca agagttggat
     3361 atcaacaggc tctctgatta cgatgttgat catatcgtgc cacagtcatt cttgaaggat
     3421 gattctatcg ataacaaggt gctcaccagg tctgataaga acaggggtaa gagtgataac
     3481 gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct cctcaacgct
     3541 aagctcatca ctcagagaaa gttcgataac ttgactaagg ctgagagggg aggactctct
     3601 gaattggata aggcaggatt catcaagagg cagcttgtgg aaaccaggca gatcactaag
     3661 cacgttgcac agatcctcga ttctaggatg aacaccaagt acgatgagaa cgataagttg
     3721 atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt cagaaaggat
     3781 ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga tgcttacctt
     3841 aacgctgttg ttggaaccgc tctcatcaag aagtatccta agctcgagtc agagttcgtg
     3901 tacggtgatt acaaggtgta cgatgtgagg aagatgatcg ctaagtctga gcaagagatc
     3961 ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt caagaccgag
     4021 attaccctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa cggtgaaaca
     4081 ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt gctctctatg
     4141 ccacaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc taaagagtct
     4201 atcctcccta agaggaactc tgataagctc attgctagga agaaggattg ggaccctaag
     4261 aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt
     4321 gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat cactatcatg
     4381 gaaaggtcat ctttcgagaa gaacccaatc gatttcctcg aggctaaggg atacaaagag
     4441 gttaagaagg atctcatcat caagctccca aagtactcac tcttcgaact cgagaacggt
     4501 agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct tgctctccca
     4561 tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa gggatctcca
     4621 gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt ggatgagatc
     4681 atcgagcaga tctctgagtt ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag
     4741 gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc agagaacatc
     4801 atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta cttcgataca
     4861 accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac cctcatccat
     4921 cagtctatca ctggactcta cgagactagg atcgatctct cacagctcgg tggtgattca
     4981 agggctgatc ctaagaagaa gaggaaggtt tgagctttcc aggcctccca gctttcgtcc
     5041 gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcccacacac
     5101 cagaatccta ctaagtttga gtattatggc attggaaaag ctgttttctt ctatcatttg
     5161 ttctgcttgt aatttactgt gttctttcag tttttgtttt cggacatcaa aatgcaaatg
     5221 gatggataag agttaataaa tgatatggtc cttttgttca ttctcaaatt attattatct
     5281 gttgttttta ctttaatggg ttgaatttaa gtaagaaagg aactaacagt gtgatattaa
     5341 ggtgcaatgt tagacatata aaacagtctt tcacctctct ttggttatgt cttgaattgg
     5401 tttgtttctt cacttatctg tgtaatcaag tttactatga gtctatgatc aagtaattat
     5461 gcaatcaagt taagtacagt ataggcttga gctccccgct actattgtct tctgcacgaa
     5521 gtggtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     5581 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     5641 tgtatgtgca tgccaaccac agggttcccc agatcaggcg ctggctgctg aacccccagc
     5701 cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc
     5761 gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca
     5821 cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg
     5881 gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt
     5941 gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc
     6001 ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg
     6061 gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt
     6121 cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca
     6181 gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg
     6241 cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac
     6301 gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc
     6361 ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg
     6421 catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt
     6481 gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc
     6541 caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt
     6601 catcgacttc gccaaacctg ccgcctcctg ttcaagacga cgcgaacgct ccacggcggc
     6661 cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt
     6721 agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt
     6781 gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct
     6841 gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca
     6901 cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata
     6961 atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt
     7021 ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg
     7081 ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc
     7141 gccggcatcg ttgcgccaca tctaggatct gccaggaacc gtaaaaaggc cgcgttgctg
     7201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8101 ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8281 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8581 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8821 ctcatactct tcctttttca atattattga agcatttatc aaggttattg tctcatgagc
     8881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg
     8941 ccagcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg
     9001 atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc
     9061 gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt
     9121 ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa
     9181 atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg
     9241 tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc
     9301 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa
     9361 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc
     9421 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg
     9481 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc
     9541 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
     9601 tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgc aaaggagatc
     9661 ctgatctgac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     9721 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     9781 aacttaataa cacattgcgg acgtttttaa tgtactgggg tggatgcagt gggccccac
//
