LOCUS       sequence-252942-       10793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH81
FEATURES             Location/Qualifiers
     source          1..10793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2023..6147
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus canis 
                     Type II CRISPR/Cas system (Chatterjee et al., 2018)"
                     /label=ScCas9
                     /note="recognizes minimal 5'-NNG-3' PAM sequences"
                     /translation="MEKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTNRKSIKK
                     NLMGALLFDSGETAEATRLKRTARRRYTRRKNRIRYLQEIFANEMAKLDDSFFQRLEES
                     FLVEEDKKNERHPIFGNLADEVAYHRNYPTIYHLRKKLADSPEKADLRLIYLALAHIIK
                     FRGHFLIEGKLNAENSDVAKLFYQLIQTYNQLFEESPLDEIEVDAKGILSARLSKSKRL
                     EKLIAVFPNEKKNGLFGNIIALALGLTPNFKSNFDLTEDAKLQLSKDTYDDDLDELLGQ
                     IGDQYADLFSAAKNLSDAILLSDILRSNSEVTKAPLSASMVKRYDEHHQDLALLKTLVR
                     QQFPEKYAEIFKDDTKNGYAGYVGIGIKHRKRTTKLATQEEFYKFIKPILEKMDGAEEL
                     LAKLNRDDLLRKQRTFDNGSIPHQIHLKELHAILRRQEEFYPFLKENREKIEKILTFRI
                     PYYVGPLARGNSRFAWLTRKSEEAITPWNFEEVVDKGASAQSFIERMTNFDEQLPNKKV
                     LPKHSLLYEYFTVYNELTKVKYVTERMRKPEFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEIIGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRHYTGWGRLSRKMINGIRDKQSGKTILDFLK
                     SDGFSNRNFMQLIHDDSLTFKEEIEKAQVSGQGDSLHEQIADLAGSPAIKKGILQTVKI
                     VDELVKVMGHKPENIVIEMARENQTTTKGLQQSRERKKRIEEGIKELESQILKENPVEN
                     TQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSVE
                     NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSEADKAGFIKRQL
                     VETRQITKHVARILDSRMNTKRDKNDKPIREVKVITLKSKLVSDFRKDFQLYKVRDINN
                     YHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKRFFY
                     SNIMNFFKTEVKLANGEIRKRPLIETNGETGEVVWNKEKDFATVRKVLAMPQVNIVKKT
                     EVQTGGFSKESILSKRESAKLIPRKKGWDTRKYGGFGSPTVAYSILVVAKVEKGKAKKL
                     KSVKVLVGITIMEKGSYEKDPIGFLEAKGYKDIKKELIFKLPKYSLFELENGRRRMLAS
                     ATELQKANELVLPQHLVRLLYYTQNISATTGSNNLGYIEQHREEFKEIFEKIIDFSEKY
                     ILKNKVNSNLKSSFDEQFAVSDSILLSNSFVSLLKYTSFGASGGFTFLDLDVKQGRLRY
                     QTVTEVLDATLIYQSITGLYETRTDLSQLGGD"
     CDS             6160..6180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6196..6448
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6496..6520
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6676..7824)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8160..8748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8240..8259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8919..9710,9711..9779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9542..9561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9780..9884)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9971..10602
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10694..10718
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctnncc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggagaa gaaatactct
     2041 attgggctgg acattggcac caattccgtt ggatgggccg tgatcactga tgattataag
     2101 gtcccctcca agaagttcaa ggttctgggt aataccaaca ggaagagcat aaaaaagaat
     2161 cttatgggcg ctctgctctt tgatagcgga gaaacggcgg aagctacacg gctcaagcgg
     2221 acagcgaggc gccgctatac caggcggaag aaccggatta gataccttca ggaaatcttc
     2281 gcgaacgaga tggccaagct cgatgactca ttcttccagc gccttgagga atcgttcctc
     2341 gttgaggagg acaaaaagaa cgagcgtcac cccatctttg gtaatctcgc ggacgaggtt
     2401 gcataccaca ggaattatcc gaccatctac cacctgagga agaagttggc ggacagcccg
     2461 gagaaggcag acctgcggct catctacctc gcgctggcgc acatcatcaa attccggggg
     2521 cattttttga tcgaagggaa actcaatgct gagaactcgg acgtggcaaa gctcttctat
     2581 cagcttatcc aaacctataa tcagctgttc gaggaatctc cgttggatga gatagaggtt
     2641 gacgctaagg gtatcctctc cgctcgtctt tcgaagtcca aacgcctgga gaaactgatc
     2701 gccgttttcc cgaacgagaa gaagaacggg ctcttcggaa acataatcgc gcttgccctg
     2761 ggactgacgc ccaacttcaa gtccaacttc gacttgacgg aggatgccaa acttcagctt
     2821 tcaaaggaca catatgacga tgatttggac gagttgctcg gccaaattgg cgaccaatac
     2881 gctgacctgt tctctgccgc aaaaaacctg tccgatgcta ttctgctgag cgatattctc
     2941 cggagcaact ccgaggtgac taaggcaccg ttgtccgcca gcatggtgaa acgctacgac
     3001 gagcaccacc aggacctggc tctcctcaag accttggtta ggcaacagtt tcccgagaag
     3061 tacgccgaaa tctttaagga tgacaccaag aacggctatg ccggttacgt tggcatcgga
     3121 atcaagcata ggaagaggac cactaaactc gccacacagg aggaattcta taaatttatc
     3181 aagcctattc tggagaagat ggacggagct gaggagctgc tcgccaagct gaacagggac
     3241 gatctcctga ggaagcagcg gacctttgat aatggctcga ttcctcatca aatccatctg
     3301 aaggagcttc atgccatact gcgcaggcaa gaagagtttt acccatttct gaaggagaat
     3361 cgggagaaga tcgaaaaaat cctgacattc cgcataccct actacgtggg cccgcttgcc
     3421 cggggaaact ctcgttttgc ttggctcacg aggaagagcg aggaggcgat aacgccatgg
     3481 aacttcgagg aggtcgtgga taaaggcgcc agcgcgcaat cgtttattga gcgtatgaca
     3541 aacttcgacg agcagctccc aaacaagaag gtgttgccaa agcacagcct cctgtacgag
     3601 tacttcacgg tttacaatga gctcaccaaa gtcaagtacg ttactgagcg tatgcgtaag
     3661 ccggagttcc tctctgggga gcagaagaag gcaatcgttg acctgctctt caaaacgaac
     3721 cgtaaggtga ccgtcaagca gctgaaggaa gattacttca aaaagatcga atgttttgac
     3781 tccgtggaga ttataggagt tgaggatagg tttaatgcct cactcggcac ctaccatgac
     3841 ttgctcaaga tcatcaaaga caaggatttc ctggacaatg aagagaacga ggatatcctt
     3901 gaggacatcg tgctcacact caccctcttc gaggaccggg agatgatcga ggagcgcctc
     3961 aagacatacg cccacctctt tgacgacaag gtgatgaaac aactcaagcg caggcactac
     4021 acgggttggg gacgcctctc ccgcaagatg attaatggta tcagggacaa acagtccggt
     4081 aagacgattc tcgatttctt gaagtccgat ggtttctcta atcgcaactt catgcagctg
     4141 atccacgacg actcactcac cttcaaggag gagattgaaa aggctcaagt gtccgggcag
     4201 ggagatagcc tccacgagca aatcgcagat ctcgccggat cgcctgcaat taagaagggc
     4261 attcttcaaa ctgtcaaaat cgtcgacgag ctggtcaagg ttatgggcca caagccagaa
     4321 aacatcgtta tcgagatggc tagggagaac cagaccacta ccaagggact ccaacagtcc
     4381 cgggagcgga agaagaggat tgaggagggg atcaaggaac tcgagtctca aattctcaag
     4441 gagaatccgg tggagaacac tcaactccag aacgagaagc tctacctgta ctacctgcag
     4501 aacggcaggg atatgtatgt cgatcaggag ctggacatca acaggctctc cgactacgac
     4561 gtcgaccata tcgtcccgca atctttcatt aaggacgact caatcgataa caaggtcctc
     4621 acgcgctccg tcgagaacag aggtaagtca gacaacgtgc cgtcggagga ggtggtgaag
     4681 aagatgaaaa actactggcg tcaactcctc aacgctaaat tgattacgca gagaaaattc
     4741 gataacttga ccaaagcgga acgcggcggt ctgtcggagg ccgacaaagc cggcttcatt
     4801 aagcggcaac tcgtggagac acgccagatc acaaagcacg tcgccaggat tcttgactcg
     4861 cggatgaata ctaaacgcga caagaacgat aagccaatca gagaagtcaa ggtgattaca
     4921 ctgaagagca aactggtcag cgacttccgc aaggatttcc agctttataa ggtgagggac
     4981 ataaacaatt atcaccacgc ccacgacgcg tacctcaacg cggtggttgg caccgcactc
     5041 attaagaaat acccgaagct cgagtcagag ttcgtctacg gcgactataa agtgtacgac
     5101 gttcggaaaa tgatagcaaa gtccgagcaa gagatcggca aggccacggc caagagattc
     5161 ttctactcca atattatgaa tttctttaag acggaggtta agttggccaa cggagagatc
     5221 cggaagcggc ctctcattga aactaatggc gagacaggcg aggtggtctg gaacaaggaa
     5281 aaggactttg ctaccgtccg caaggtcctc gcgatgccgc aagtgaacat tgtcaaaaag
     5341 acggaagttc agacaggcgg gttctcgaag gagtcaatct tgtcgaagcg tgagtccgcc
     5401 aagctcatcc cacgcaagaa ggggtgggat acgcggaagt atggaggttt cgggtccccc
     5461 actgttgcat actcaattct cgtggtcgcc aaggtggaga agggaaaagc caagaagctc
     5521 aaaagcgtga aggtcctcgt gggtataacg ataatggaaa agggtagcta cgagaaggac
     5581 ccaatcggtt tcctcgaagc caaggggtac aaagatatca aaaaggagct tatattcaaa
     5641 ctgccgaagt actcattgtt tgagctggag aacggcaggc ggcggatgtt ggcctcggcg
     5701 acagaactgc aaaaggcgaa cgagctcgtc ttgcctcagc atctcgttag gcttctctac
     5761 tacacccaaa atatctctgc gactacaggg tctaacaacc tcggttacat cgagcagcat
     5821 agggaggaat tcaaggagat ctttgagaag atcatcgact tctccgagaa gtacatcctt
     5881 aagaacaaag tgaactccaa cttgaagtcg agcttcgacg agcagttcgc tgtttccgat
     5941 tctattctcc tgtcgaactc attcgtgtca cttctgaagt acacgtcgtt cggcgcatct
     6001 ggcggcttta ccttcctcga cctcgacgtg aagcaaggcc ggctccgcta ccaaaccgtg
     6061 accgaggttc tcgacgcgac attgatctac cagtccatta ccggcctgta cgaaacgagg
     6121 acggacctct cacagctcgg aggggactct agagcggacc caaagaagaa gcgcaaggtg
     6181 taggcttgtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     6241 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     6301 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     6361 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     6421 cgcgcggtgt catctatgtt actagatcga cgctactatt gtcttctgca cgaagtggtt
     6481 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     6541 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     6601 tgcatgccaa ccacagggtt ccccagatca ggcgctggct gctgaacccc cagccggaac
     6661 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     6721 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     6781 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     6841 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     6901 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     6961 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     7021 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     7081 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     7141 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     7201 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     7261 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     7321 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     7381 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     7441 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
     7501 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     7561 cttcgccaaa cctgccgcct cctgttcaag acgacgcgaa cgctccacgg cggccgatgg
     7621 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     7681 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     7741 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     7801 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
     7861 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
     7921 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
     7981 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
     8041 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
     8101 atcgttgcgc cacatctagg atctgccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9061 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9781 ctcttccttt ttcaatatta ttgaagcatt tatcaaggtt attgtctcat gagcggatac
     9841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacgaa ttggccagcg
     9901 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
     9961 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
    10021 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
    10081 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
    10141 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
    10201 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
    10261 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
    10321 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
    10381 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
    10441 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
    10501 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
    10561 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcgcaaagga gatcctgatc
    10621 tgactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    10681 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    10741 ataacacatt gcggacgttt ttaatgtact ggggtggatg cagtgggccc cac
//
