LOCUS       sequence-252943-       10772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::xCas9 3.7:NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH80
FEATURES             Location/Qualifiers
     source          1..10772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2023..6126
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFI
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6139..6159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6175..6427
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6475..6499
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6655..7803)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8139..8727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8219..8238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8898..9689,9690..9758))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9521..9540
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9759..9863)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9950..10581
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10673..10697
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggacaa gaagtactcc
     2041 attgggctcg atatcggcac aaacagcgtc ggctgggccg tcattacgga cgagtacaag
     2101 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
     2161 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga
     2221 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
     2281 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
     2341 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
     2401 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
     2461 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
     2521 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
     2581 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
     2641 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
     2701 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
     2761 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatactaa gcttcaactg
     2821 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
     2881 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg
     2941 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gctgtatgat
     3001 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag
     3061 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga
     3121 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc
     3181 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac
     3241 aatggaatca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa
     3301 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt
     3361 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact
     3421 cgcaaatcag aagagactat cactccctgg aacttcgaga aggtcgtgga taagggggcc
     3481 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag
     3541 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag
     3601 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga tcagaagaaa
     3661 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa
     3721 gattatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc
     3781 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc
     3841 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt
     3901 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa
     3961 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg
     4021 atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct taagtccgat
     4081 ggatttgcca accggaactt catccagttg atccatgatg actctctcac ctttaaggag
     4141 gacatccaga aagcacaagt ttctggccag ggggacagtc tccacgagca catcgctaat
     4201 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa
     4261 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag
     4321 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag
     4381 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt
     4441 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag
     4501 gaactggaca tcaatcggct ctccgactac gacgtggatc atatcgtgcc ccagtctttt
     4561 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag
     4621 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg
     4681 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt
     4741 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag
     4801 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat
     4861 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ttcagatttc
     4921 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat
     4981 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct
     5041 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag
     5101 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc
     5161 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac
     5221 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc
     5281 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc
     5341 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg
     5401 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg
     5461 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc
     5521 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga
     5581 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt
     5641 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgtac tgcagaaagg taacgagctg
     5701 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa
     5761 ggatctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt
     5821 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac
     5881 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca
     5941 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac
     6001 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca
     6061 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt
     6121 ggagacagca gggctgaccc caagaagaag aggaaggtgt aggcttgtca agcagatcgt
     6181 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     6241 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     6301 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     6361 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     6421 ctagatcgac gctactattg tcttctgcac gaagtggttt aaactatcag tgtttgacag
     6481 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     6541 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     6601 cccagatcag gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg
     6661 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa
     6721 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc
     6781 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata
     6841 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt
     6901 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga
     6961 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg
     7021 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc
     7081 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa
     7141 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca
     7201 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt
     7261 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt
     7321 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg
     7381 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa
     7441 cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt
     7501 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc
     7561 ctgttcaaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc
     7621 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga
     7681 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc
     7741 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt
     7801 cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg
     7861 atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt
     7921 cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa
     7981 taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt
     8041 gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctagga
     8101 tctgccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8161 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8221 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8281 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8341 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8401 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8461 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8521 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8581 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8641 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8701 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8761 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8821 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8881 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8941 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9001 ggcttaccat ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca
     9061 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9121 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9181 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9241 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9301 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9361 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9421 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9481 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9541 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9601 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9661 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9721 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9781 tgaagcattt atcaaggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9841 aataaacaaa taggggttcc gcgcacgaat tggccagcgc tgccattttt ggggtgaggc
     9901 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg
     9961 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
    10021 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
    10081 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
    10141 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
    10201 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
    10261 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg
    10321 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
    10381 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
    10441 agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc
    10501 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    10561 gcaaccagcc cggtgagcgt cgcaaaggag atcctgatct gactgatggg ctgcctgtat
    10621 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
    10681 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
    10741 taatgtactg gggtggatgc agtgggcccc ac
//
